SOX9 antibody | 3C10
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX9 expressing cells in human corneoscleral tissue sections by immunofluorescence.
Image caption:
Immunohistochemical localization of SoxE family members in corneoscleral tissue sections.
Immunofluorescence microscopy demonstrates nuclear staining for Sox8 (clone 4E4.1) and Sox9 (clone 3C10) in suprabasal epithelial cells at the limbus (left column) and central cornea (right column), whereas Sox10 (clone BC34) is confined to few cells in the basal limbal epithelium. Higher magnification images of basal limbal regions (middle column), as indicated by boxed areas, show differential cytoplasmic (arrows) and nuclear localization of Sox8 and Sox9 in basal and suprabasal limbal epithelial cells.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U.
Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells.
Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX9 expressing cells in human corneoscleral tissue sections by immunofluorescence.
Image caption:
Immunohistochemical localization of SoxE family members in corneoscleral tissue sections. (B) High magnification images of individual channels show cytoplasmic localization of Sox9 in basal stem/progenitor cell clusters and nuclear localization in suprabasal limbal epithelial cells.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U.
Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells.
Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX9 expressing cells in human corneoscleral tissue sections by immunofluorescence.
Image caption:
Immunohistochemical localization of SoxE family members in corneoscleral tissue sections. Double labeling experiments show nuclear co-localization of Sox8 (rabbit IgG) and Sox9 (clone 3C10) (left), distinct localization of Sox9 (clone 3C10) and Sox10 (rabbit IgG) (middle), and localization of Sox10 (clone BC34) to Melan A-positive cells (right). Nuclear counterstaining: DAPI.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U.
Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells.
Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX 9 expressing cells in limbal epithelium by immunofluorescence.
Image caption:
Co-localisation of Sox9 with markers related to progenitor cell phenotype, differentiation and proliferation in the limbal epithelium. Double-labelling demonstrates co-localisation (arrows) of cytoplasmic Sox9 (red) with the stem/progenitor cell markers (green) p63α and Oct4 in basal epithelial cells at the limbus. Suprabasal epithelial cells revealed co-localisation of nuclear Sox9 (red) with differentiation-related markers (green) Pax6 as well as proliferation-related marker Ki-67. Sox9 monoclonal mouse antibody (clone 3C10) was used for double labelling experiments with polyclonal antibodies against Oct4, p63α, Pax6 and Ki-67, Nuclear counterstaining: DAPI.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX 9 expressing cells in limbal epithelium by immunofluorescence.
Image caption:
Expression of Sox9 during limbal epithelial cell expansion and wound healing in vitro. Limbal epithelial cell (LEPC) clones (dashed lines) on 3T3 feeder cells (3T3) stain positively for Sox9 (clone 3C10, red), preferentially towards the proliferating border of the clones; Sox9-expressing cells partly co-localize with Ki-67 (green). Nuclear staining: DAPI.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX 9 expressing cells in limbal epithelium by immunofluorescence.
Image caption:
Expression of Sox9 during limbal epithelial cell expansion and wound healing in vitro.
Immunofluorescent staining shows increased levels of nuclear Sox9 (clone 3C10) in basal/suprabasal limbal epithelial cells and central corneal epithelial cells after epithelial debridement and regeneration compared to unwounded control corneas (the background fluorescence seen in the central corneal stroma may be attributed to the epithelial debridement allowing media and serum components to infiltrate the stroma during the wound healing process). Higher magnification images of limbal epithelial progenitor cell clusters show increased nuclear localization of Sox9 in basal epithelial cells in wound healing conditions compared to cytoplasmic retention of Sox9 in control tissues (inserts). Nuclear staining: DAPI.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX 9 expressing cells in limbal epithelium by western blotting.
Image caption:
Interactions between Sox9 and cell signaling pathways.
Changes in Sox9 protein expression following exposure of cultured limbal epithelial cells to Hedgehog signaling activators Sonic hedgehog (SHH), SAG and Purmorphamine (Purmo); BMP-2; Wnt signaling activators Wnt-3a, lithium chloride (LiCl) and IM-12; and Notch signaling ligand JAG-1 for 48 hours relative to vehicle-treated control cells (Ctrl). Sox9 protein expression was detected with the monoclonal antibody (clone 3C10), normalized to the house-keeping gene β-actin, and expressed as percent of the expression in control cells (Ctrl); (n = 3; mean ±SD).
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9 antibody, clone 3C10 (MCA4974Z) used for the identification of SOX 9 expressing cells in limbal epithelium by western blotting following SOX9 RNAi knockdown.
Image caption:
Changes in protein expression following knockdown of SOX9 in cultured limbal epithelial cells by RNAi. (A) Western blot analysis of limbal epithelial cells transfected with either siRNA that targets SOX9 or non-targeting, scrambled siRNA as a control. Protein expression of Sox9(n = 3; mean ± SD); *p < 0.05, **p < 0.005, ***p < 0.0001, unpaired t-test.
From: Menzel-Severing J, Zenkel M, Polisetti N, Sock E, Wegner M, Kruse FE, Schlötzer-Schrehardt U. Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells. Sci Rep. 2018 Jul 6;8(1):10268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human SOX9:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 3C10
- Isotype
- IgG2a
- Specificity
- SOX9
Antibody Quality and Performance Guarantee
| Mouse anti Human SOX9 antibody, clone 3C10 recognizes human Transcription factor SOX-9, also known SOX9, a 509 amino acid ~56 kDa transcription factor. Mutation of the SOX9 gene can lead to development of Campomelic dysplasia (CMD1) an osteochondrodysplasia with bowing and angulation of the long bones along with other skeletal malformations (Kwok et al. 1995). Mutation can also lead to 46,XX sex reversal 2 (SRXX2) presenting with male gonads in a genetic female (Cox et al. 2011) or 46,XY sex reversal 10 (SRXY10) with gonadal dysgensis (Kim et al. 2015). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 400-510 of human SOX9
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Immunohistology - Paraffin | |
0.1 | 10 ug/ml |
| Western Blotting | |
0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for SOX9 antibody
-
Menzel-severing, J. et al. (2018) Transcription factor profiling identifies Sox9 as regulator of proliferation and differentiation in corneal epithelial stem/progenitor cells.
Sci Rep. 8 (1): 10268.
- RRID
- AB_2194161
- UniProt
- P48436
- Entrez Gene
- SOX9
- GO Terms
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0005634 nucleus
MCA4974Z
149406
157905
168689
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