SNAP-25 antibody | SP12

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Mouse anti Human SNAP-25

Product Type
Monoclonal Antibody
Clone
SP12
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1308 E P WB 0.2 mg
Mouse anti Human SNAP-25 antibody, clone SP12 recognizes the human pre-synaptic protein, SNAP-25, also known as Synaptosomal-associated protein 25, Super protein (SUP) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid  presynaptic protein of ~25 kDa containing two t-SNARE coiled-coil homology domains. Mouse anti human SNAP-25 antibody, clone SP12 will recognize SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems.

Mouse anti Human SNAP-25 antibody, clone SP12  has been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (Honer et al. 1994).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Pig
Rat
Gerbil
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Crude human synaptic immunoprecipitate.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P60880 Related reagents
Entrez Gene
SNAP25 Related reagents
Regulatory
For research purposes only

Applications of SNAP-25 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1/2000 1/5000
Western Blotting 1/500 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Brain

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

ELISA

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for SNAP-25 antibody

  1. Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
    Acta Neuropathol. 87 (2): 202-10.
  2. Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
    Neurobiol Aging. 13 (3): 375-82.
  3. Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
    Brain Res. 609 (1-2): 9-20.
  4. Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
    Biol Psychiatry. 31 (2): 147-58.
  5. Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
    Brain Res. 500 (1-2): 379-83.
  6. Masliah, E. et al. (1994) Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus.
    Acta Neuropathol. 87 (2): 135-42.
  7. Simpson, I.A. et al. (1994) Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease.
    Ann Neurol. 35 (5): 546-51.
  8. Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampal-entorhinal formation in Alzheimer's disease with and without Lewy body disease.
    Brain Res. 667 (1): 24-32.
  9. Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (≥80 years of age) humans.
    Acta Neuropathol. 88 (3): 212-21.
  10. Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
    Neurobiol Aging. 16 (3): 285-98; discussion 298-304.
  11. Bragina, L. et al. (2006) GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation.
    J Neurochem. 99: 134-41.
  12. Steel, G.J. et al. (1997) Evidence for interaction of the fusion protein alpha-SNAP with membrane lipid.
    Biochem J. 325 : 511-8.
  13. Reed, G.L. et al. (1999) Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion.
    Blood. 93: 2617-26.
  14. Vannucchi, M.G. et al. (2000) Synapse formation during neuron differentiation: an in situ study of the myenteric plexus during murine embryonic life.
    J Comp Neurol. 425: 369-81.
  15. Lemons, P.P. et al. (1997) Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery.
    Blood. 90: 1490-500.
  16. Mukaetova-Ladinska, E.B. et al. (2000) Staging of cytoskeletal and beta-amyloid changes in human isocortex reveals biphasic synaptic protein response during progression of Alzheimer's disease.
    Am J Pathol. 157: 623-36.
  17. Sawada, K. et al. (2002) Altered immunoreactivity of complexin protein in prefrontal cortex in severe mental illness.
    Mol Psychiatry. 7: 484-92.
  18. Chen, H.Y. et al. (2009) Melatonin improves presynaptic protein, SNAP-25, expression and dendritic spine density and enhances functional and electrophysiological recovery following transient focal cerebral ischemia in rats.
    J Pineal Res. 47: 260-70.
  19. Ishimaru, H. et al. (2001) Changes in presynaptic proteins, SNAP-25 and synaptophysin, in the hippocampal CA1 area in ischemic gerbils.
    Brain Res. 903: 94-101.