SNAP-25 antibody | SP12

Mouse anti Human SNAP-25

Product Type
Monoclonal Antibody
Clone
SP12
Isotype
IgG1
Specificity
SNAP-25

Product Code Applications Pack Size List Price Your Price Qty
MCA1308
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P WB 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human SNAP-25 antibody, clone SP12 recognizes the human pre-synaptic protein, SNAP-25, also known as Synaptosomal-associated protein 25, Super protein (SUP) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid  presynaptic protein of ~25 kDa containing two t-SNARE coiled-coil homology domains. Mouse anti human SNAP-25 antibody, clone SP12 will recognize SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems.

Mouse anti Human SNAP-25 antibody, clone SP12  has been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (Honer et al. 1994).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Hamster
Pig
Rat
Gerbil
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Crude human synaptic immunoprecipitate.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NSO myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1/2000 1/5000
Western Blotting 1/500 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Brain

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for SNAP-25 antibody

  1. (2011) Human Protein Atlas: CAB000360
    Human Protein Atlas: CAB000360
  2. Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
    Acta Neuropathol. 87 (2): 202-10.
  3. Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
    Neurobiol Aging. 13 (3): 375-82.
  4. Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
    Brain Res. 609 (1-2): 9-20.
  5. Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
    Biol Psychiatry. 31 (2): 147-58.
  6. Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
    Brain Res. 500 (1-2): 379-83.
  7. Sawada, K. et al. (2002) Altered immunoreactivity of complexin protein in prefrontal cortex in severe mental illness.
    Mol Psychiatry. 7: 484-92.
  8. Ishimaru, H. et al. (2001) Changes in presynaptic proteins, SNAP-25 and synaptophysin, in the hippocampal CA1 area in ischemic gerbils.
    Brain Res. 903: 94-101.
  9. View The Latest Product References
  10. Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampal-entorhinal formation in Alzheimer's disease with and without Lewy body disease.
    Brain Res. 667 (1): 24-32.
  11. Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (≥80 years of age) humans.
    Acta Neuropathol. 88 (3): 212-21.
  12. Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
    Neurobiol Aging. 16 (3): 285-98; discussion 298-304.
  13. Bragina, L. et al. (2006) GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation.
    J Neurochem. 99: 134-41.
  14. Steel, G.J. et al. (1997) Evidence for interaction of the fusion protein alpha-SNAP with membrane lipid.
    Biochem J. 325 : 511-8.
  15. Reed, G.L. et al. (1999) Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion.
    Blood. 93: 2617-26.
  16. Vannucchi, M.G. et al. (2000) Synapse formation during neuron differentiation: an in situ study of the myenteric plexus during murine embryonic life.
    J Comp Neurol. 425: 369-81.
  17. Lemons, P.P. et al. (1997) Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery.
    Blood. 90: 1490-500.
  18. Mukaetova-Ladinska, E.B. et al. (2000) Staging of cytoskeletal and beta-amyloid changes in human isocortex reveals biphasic synaptic protein response during progression of Alzheimer's disease.
    Am J Pathol. 157: 623-36.
  19. Chen, H.Y. et al. (2009) Melatonin improves presynaptic protein, SNAP-25, expression and dendritic spine density and enhances functional and electrophysiological recovery following transient focal cerebral ischemia in rats.
    J Pineal Res. 47: 260-70.

ELISA

Immunohistology - Paraffin

Western Blotting

Synonyms
SYNAPTOSOMAL-ASSOCIATED Protein
RRID
AB_322417
UniProt
P60880
Entrez Gene
SNAP25
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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