SNAP-25 antibody | SP12
Mouse anti Human SNAP-25 antibody, clone SP12 has been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (Honer et al. 1994).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Hamster Pig Rat Gerbil
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Crude human synaptic immunoprecipitate.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NSO myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
Applications of SNAP-25 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin||1/2000||1/5000|
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
- Histology Positive Control Tissue
Secondary Antibodies Available
Product Specific References
References for SNAP-25 antibody
Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.
Acta Neuropathol. 87 (2): 202-10.
Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.
Neurobiol Aging. 13 (3): 375-82.
Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.
Brain Res. 609 (1-2): 9-20.
Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.
Biol Psychiatry. 31 (2): 147-58.
Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.
Brain Res. 500 (1-2): 379-83.
Sawada, K. et al. (2002) Altered immunoreactivity of complexin protein in prefrontal cortex in severe mental illness.
Mol Psychiatry. 7: 484-92.
Ishimaru, H. et al. (2001) Changes in presynaptic proteins, SNAP-25 and synaptophysin, in the hippocampal CA1 area in ischemic gerbils.
Brain Res. 903: 94-101.
Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampal-entorhinal formation in Alzheimer's disease with and without Lewy body disease.
Brain Res. 667 (1): 24-32.
Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (≥80 years of age) humans.
Acta Neuropathol. 88 (3): 212-21.
Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.
Neurobiol Aging. 16 (3): 285-98; discussion 298-304.
Bragina, L. et al. (2006) GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation.
J Neurochem. 99: 134-41.
Steel, G.J. et al. (1997) Evidence for interaction of the fusion protein alpha-SNAP with membrane lipid.
Biochem J. 325 : 511-8.
Reed, G.L. et al. (1999) Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion.
Blood. 93: 2617-26.
Vannucchi, M.G. et al. (2000) Synapse formation during neuron differentiation: an in situ study of the myenteric plexus during murine embryonic life.
J Comp Neurol. 425: 369-81.
Lemons, P.P. et al. (1997) Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery.
Blood. 90: 1490-500.
Mukaetova-Ladinska, E.B. et al. (2000) Staging of cytoskeletal and beta-amyloid changes in human isocortex reveals biphasic synaptic protein response during progression of Alzheimer's disease.
Am J Pathol. 157: 623-36.
Chen, H.Y. et al. (2009) Melatonin improves presynaptic protein, SNAP-25, expression and dendritic spine density and enhances functional and electrophysiological recovery following transient focal cerebral ischemia in rats.
J Pineal Res. 47: 260-70.
Fluorescent SpectraviewerWatch the Tool Tutorial Video ▸
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra