SLP-76 antibody | SLP-76/03

Mouse anti SLP-76

Product Type
Monoclonal Antibody
Clone
SLP-76/03
Isotype
IgG2b
Specificity
SLP-76

Product Code Applications Pack Size List Price Your Price Qty
MCA2377
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti SLP-76 antibody, clone SLP-76/03 recognises the SH2 domain-containing leucocyte protein, also known as SLP-76 or Lymphocyte cytosolic protein 2. SLP-76 is a ~76 kDa tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage.

SLP-76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through GRB2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP-76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
A fusion protein corresponding to amino acids 216-434 of human SLP-76.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2377 detects a band of approximately 76kDa in T lymphocyte cell lysates.

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References for SLP-76 antibody

  1. Di Bartolo V et al. (2007) A novel pathway down-modulating T cell activation involves HPK-1-dependent recruitment of 14-3-3 proteins on SLP-76.
    J Exp Med. 204 (3): 681-91.
  2. Brockmeyer, C. et al. (2011) T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies themis as a new TCR signalosome component.
    J Biol Chem. 286: 7535-47.
  3. Lasserre, R. et al. (2011) Release of serine/threonine-phosphorylated adaptors from signaling microclusters down-regulates T cell activation.
    J Cell Biol. 195 (5): 839-53.
  4. Lasserre, R. et al. (2010) Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.
    EMBO J. 29: 2301-14.
  5. Paster, W. et al. (2013) GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development.
    J Immunol. 190 (7): 3749-56.

Further Reading

  1. Bonilla, F.A. et al. (2000) Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcgamma receptors I and II/III.
    Proc Natl Acad Sci U S A. 97 (4): 1725-30.

Immunofluorescence

Western Blotting

RRID
AB_2136892
UniProt
Q13094
Entrez Gene
LCP2
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0006955 immune response
GO:0030168 platelet activation
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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