SLP-76 antibody | SLP-76/03
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Mouse anti SLP-76
- Product Type
- Monoclonal Antibody
- Clone
- SLP-76/03
- Isotype
- IgG2b
- Specificity
- SLP-76
Antibody Quality and Performance Guarantee
| Mouse anti SLP-76 antibody, clone SLP-76/03 recognises the SH2 domain-containing leucocyte protein, also known as SLP-76 or Lymphocyte cytosolic protein 2. SLP-76 is a ~76 kDa tyrosine phosphoprotein, expressed primarily by T lymphocytes and cells of the myeloid lineage. SLP-76 is one of several adaptor proteins assisting with TCR-mediated signal transduction, through GRB2 binding, functioning as a substrate for the protein-tyrosine kinase ZAP-70 and thus playing a crucial role in both T cell ontogeny and activation. Other molecules that associate with SLP-76 include phospholipase C (PLC)–gamma, Vav, Nck and fyn binding protein. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- < 0.1% sodium azide (NaN3)
- Immunogen
- A fusion protein corresponding to amino acids 216-434 of human SLP-76.
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti Human SLP-76 antibody, clone SLP-76/03 detects a band of approximately 76 kDa in T lymphocyte cell lysates.
References for SLP-76 antibody
-
Di Bartolo V et al. (2007) A novel pathway down-modulating T cell activation involves HPK-1-dependent recruitment of 14-3-3 proteins on SLP-76.
J Exp Med. 204 (3): 681-91. -
Brockmeyer, C. et al. (2011) T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies themis as a new TCR signalosome component.
J Biol Chem. 286: 7535-47. -
Lasserre, R. et al. (2011) Release of serine/threonine-phosphorylated adaptors from signaling microclusters down-regulates T cell activation.
J Cell Biol. 195 (5): 839-53. -
Lasserre, R. et al. (2010) Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse.
EMBO J. 29: 2301-14. -
Paster, W. et al. (2013) GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development.
J Immunol. 190 (7): 3749-56.
Further Reading
-
Bonilla, F.A. et al. (2000) Adapter proteins SLP-76 and BLNK both are expressed by murine macrophages and are linked to signaling via Fcgamma receptors I and II/III.
Proc Natl Acad Sci U S A. 97 (4): 1725-30.
- RRID
- AB_2136892
- UniProt
- Q13094
- Entrez Gene
- LCP2
- GO Terms
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0006955 immune response
- GO:0030168 platelet activation
- GO:0050852 T cell receptor signaling pathway
MCA2377
160121
170511
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