SC5b-9 antibody | 056B-75.2.3.10 (3R2/0)

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E ResetMouse anti Human SC5b-9
- Product Type
- Monoclonal Antibody
- Clone
- 056B-75.2.3.10 (3R2/0)
- Isotype
- IgG2a
- Specificity
- SC5b-9
Mouse anti Human SC5b-9 antibody, clone 056B-75.2.3.10 (3R2/0) recognizes the SC5b-9 complex of around 330 kDa. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation. Removal of Sodium Azide is recommended prior to use in functional assays. Mouse anti Human SC5b-9 antibody, clone 3R2/0 has been reported to work in western blotting applications. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.176 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
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Flow Cytometry | ![]() |
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Immunohistology - Frozen | ![]() |
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Western Blotting | ![]() |
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Description | Mouse IgG2a Negative Control |
References for SC5b-9 antibody
-
Baksmeier, C. et al. (2021) Modified recombinant human IgG1-Fc is superior to natural intravenous immunoglobulin at inhibiting immune-mediated demyelination.
Immunology. 164 (1): 90-105.
Further Reading
-
Biesecker, G. (1990) The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor.
J Immunol. 145 (1): 209-14. -
Greenstein, J.D. et al. (1995) The kinetics and distribution of C9 and SC5b-9 in vivo: effects of complement activation.
Clin Exp Immunol. 100 (1): 40-6. -
Rus, H. et al. (2005) C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection.
Ann Med. 37 (2): 97-104.
- RRID
- AB_844532
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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