SC5b-9 antibody | 056B-75.2.3.10 (3R2/0)
Mouse anti Human SC5b-9 antibody, clone 056B-75.2.3.10 (MCA2611) used as a detection reagent in an ELISA to evaluatre Fc binding and activation.
Image caption:
Complement binding by Fc mutants. Complement binding and activation was assessed by ELISA on Fc coated wells.
Serial dilutions of Fc from 10 μg/ml were coated onto maxi-sorb 96 ELISA plates at 100 μl/well in 0.05M carbonate buffer overnight at 4oC. Following washing with PBST (PBS-Tween-20 0.05%), plates were incubated with human serum diluted 1/100 in gelatin veronal buffer for 1h at room temperature. After washing in PBST, plates were incubated with a 1/500 dilution of mouse anti-human C5b-9 mAb (Serotec: MCA2611) in PBST supplemented with 0.5% w/v BSA for 2h. For detection of the C5b-9 binding mAb, plates were washed as before prior to incubation for 2h with a 1:500 dilution of anti-mouse IgG2b-HRP. Plates were then washed ten times in PBST before developing for HRP substrate 3,3’,5,5’-tetramethyl-benzidine dihydrochloride in phosphate-citrate buffer. Reactions were stopped with 25μl 2M H2SO4 and plates read on a plate reader at OD450nm. Duplicate experiments are shown.
From:Baksmeier C, Blundell P, Steckel J, Schultz V, Gu Q, Da Silva Filipe A, Kohl A, Linnington C, Lu D, Dell A, Haslam S, Wang J, Czajkowsky D, Goebels N, Pleass RJ.
Modified recombinant human IgG1-Fc is superior to natural intravenous immunoglobulin at inhibiting immune-mediated demyelination.
Immunology. 2021 Sep;164(1):90-105.
doi: 10.1111/imm.13341.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human SC5b-9
- Product Type
- Monoclonal Antibody
- Clone
- 056B-75.2.3.10 (3R2/0)
- Isotype
- IgG2a
- Specificity
- SC5b-9
Antibody Quality and Performance Guarantee
| Mouse anti Human SC5b-9 antibody, clone 056B-75.2.3.10 (3R2/0) recognizes the SC5b-9 complex of around 330 kDa. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation. Removal of Sodium Azide is recommended prior to use in functional assays. Mouse anti Human SC5b-9 antibody, clone 3R2/0 has been reported to work in western blotting applications. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.18 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunohistology - Frozen | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for SC5b-9 antibody
-
Baksmeier, C. et al. (2021) Modified recombinant human IgG1-Fc is superior to natural intravenous immunoglobulin at inhibiting immune-mediated demyelination.
Immunology. 164 (1): 90-105.
Further Reading
-
Biesecker, G. (1990) The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor.
J Immunol. 145 (1): 209-14. -
Greenstein, J.D. et al. (1995) The kinetics and distribution of C9 and SC5b-9 in vivo: effects of complement activation.
Clin Exp Immunol. 100 (1): 40-6. -
Rus, H. et al. (2005) C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection.
Ann Med. 37 (2): 97-104.
- RRID
- AB_844532
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