RelB antibody | 17.3
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WB IF ResetMouse anti RelB
- Product Type
- Monoclonal Antibody
- Clone
- 17.3
- Isotype
- IgG1
- Specificity
- RelB
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| Mouse anti-RelB antibody, clone 17.3 recognizes the transcription factor RelB, also known as I-Rel. RelB is encoded by the v-rel avian reticuloendotheliosis viral oncogene homolog B gene and is a critical component of the NF-kappa-B pathway. Upon cleavage of the NF-kappa-B p105 and p100 subunits into the NF-kappa-B p50 and p52 subunits, RelB forms RelB-p50 and RelB-p52 heterodimers. These dimers act as activators of transcription by binding to kappaB sites present on DNA (Oeckinghaus and Ghosh 2009). RelB-p50 and RelB-p52 binding induces the expression of genes mediating immune responses, inflammation and apoptosis (Jing and Lee 2014). Misregulation of the NF-kappa-B pathway has been implicated in a variety of disease states including autoimmunity and cancer. As the NF-kappa-B pathway has been demonstrated to be constitutively active in a large group of tumors, developing NF-kappa-B inhibitors has emerged as a new cancer therapy approach (Chaturverdi et al. 2011 and Baud and Karin et al. 2009). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant protein derived from C-terminal region of human RelB protein
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunocytochemistry |  |
1 ug/ml | |
| Immunofluorescence | |
1 ug/ml | |
| Immunohistology - Paraffin | |
1/10 | 1/50 |
| Immunoprecipitation | |
||
| Western Blotting | |
1 - 2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti RelB antibody recognizes a band of approximately 63 kDa in THP-1 cell lysate, Daudi cell lysate, and Raji cell lysate
- Synonyms
- Irel
- I-Rel
- UniProt
- Q01201
- Entrez Gene
- RELB
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003714 transcription corepressor activity
- GO:0005634 nucleus
- GO:0006355 regulation of transcription, DNA-dependent
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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