Podoplanin antibody | 4D5aE5E6
Mouse anti Human Podoplanin
- Product Type
- Monoclonal Antibody
|Mouse anti Human podoplanin antibody, clone 4D5aE5E6 recognizes human podoplanin also known as gp36, Aggrus, PA2.26 antigen or T1 alpha. Podoplanin is a 140 amino acid ~36 kDa single pass, type I cell membrane-associated glycoprotein, with a broad spectrum of distribution. Podoplanin is strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver.
Podoplanin is preferentially expressed on lymphatic and absent on blood endothelium (Breiteneder-Geleff et al. 1999) and therefore Mouse anti Human podoplanin antibody, clone 4D5aE5E6 is readily able to distinguish between lymphatic vessels and blood vasculature. Podoplanin is reported to be a reliable indicator of tumour lymphangiogenesis (Kitano et al. 2010).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- < 0.1% sodium azide (NaN3)
- ORF Human podoplanin ectodomain expressed in E.coli.
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||0.75ug/ml||2ug/ml|
|Immunohistology - Paraffin 1|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human lung
References for Podoplanin antibody
Furtado, G.C. et al. (2007) Lymphotoxin beta receptor signaling is required for inflammatory lymphangiogenesis in the thyroid.
Proc Natl Acad Sci U S A. 104 (12): 5026-31.
Pardo, J. et al. (2007) Pseudomesotheliomatous carcinoma of the lung with a distinct morphology, immunohistochemistry, and comparative genomic hybridization profile.
Ann Diagn Pathol. 11 (4): 241-51.
Link, A. et al. (2011) Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans.
Am J Pathol. 178 (4): 1662-75.
Gu, Y. et al. (2008) Lymphangiogenesis induced by VEGF-C and VEGF-D promotes metastasis and a poor outcome in breast carcinoma: a retrospective study of 61 cases.
Clin Exp Metastasis. 25 (7): 717-25.
Ra, S.H. et al. (2007) Recurrent hepatic lymphangiomatosis after orthotopic liver transplantation.
Liver Transpl. 13 (11): 1593-7.
Matsui, K. et al. (2003) Lymphatic microvessels in the rat remnant kidney model of renal fibrosis: aminopeptidase p and podoplanin are discriminatory markers for endothelial cells of blood and lymphatic vessels.
J Am Soc Nephrol. 14 (8): 1981-9.
Dadras, S.S. et al. (2003) Tumor lymphangiogenesis: a novel prognostic indicator for cutaneous melanoma metastasis and survival.
Am J Pathol. 162 (6): 1951-60.
Kato, Y. et al. (2003) Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors.
J Biol Chem. 278 (51): 51599-605.
Schacht, V. et al. (2003) T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema.
EMBO J. 22 (14): 3546-56.
Zimmer, G. et al. (1999) Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium.
Biochem J. 341 ( Pt 2): 277-84.
- Entrez Gene
- GO Terms
- GO:0000902 cell morphogenesis
- GO:0001946 lymphangiogenesis
- GO:0005887 integral to plasma membrane
- GO:0008360 regulation of cell shape
- GO:0031258 lamellipodium membrane
- GO:0031527 filopodium membrane
- GO:0031528 microvillus membrane
- GO:0032587 ruffle membrane
MCA2370146901 154066 161940
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