Podoplanin antibody | 4D5aE5E6

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Mouse anti Human Podoplanin

Product Type
Monoclonal Antibody
Clone
4D5aE5E6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2370 C P* 0.1 mg
Mouse anti Human podoplanin antibody, clone 4D5aE5E6 recognizes human podoplanin also known as gp36, Aggrus, PA2.26 antigen or T1 alpha. Podoplanin is a 140 amino acid ~36 kDa single pass, type I cell membrane-associated glycoprotein, with a broad spectrum of distribution. Podoplanin is strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver.

Podoplanin is preferentially expressed on lymphatic and absent on blood endothelium (Breiteneder-Geleff et al. 1999) and therefore Mouse anti Human podoplanin antibody, clone 4D5aE5E6 is readily able to distinguish between lymphatic vessels and blood vasculature. Podoplanin is reported to be a reliable indicator of tumour lymphangiogenesis (Kitano et al. 2010).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
ORF Human podoplanin ectodomain expressed in E.coli.
Approx. Protein Concentrations
IgG concentration 1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q86YL7 Related reagents
Entrez Gene
PDPN Related reagents
GO Terms
GO:0000902 cell morphogenesis
GO:0001946 lymphangiogenesis
GO:0005887 integral to plasma membrane
GO:0008360 regulation of cell shape
GO:0031258 lamellipodium membrane
GO:0031527 filopodium membrane
GO:0031528 microvillus membrane
GO:0032587 ruffle membrane
Regulatory
For research purposes only

Applications of Podoplanin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 0.75ug/ml 2ug/ml
Immunohistology - Paraffin 1
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Normal lung

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Product Specific References

References for Podoplanin antibody

  1. Furtado, G.C. et al. (2007) Lymphotoxin beta receptor signaling is required for inflammatory lymphangiogenesis in the thyroid.
    Proc Natl Acad Sci U S A. 104 (12): 5026-31.
  2. Pardo, J. et al. (2007) Pseudomesotheliomatous carcinoma of the lung with a distinct morphology, immunohistochemistry, and comparative genomic hybridization profile.
    Ann Diagn Pathol. 11 (4): 241-51.
  3. Link, A. et al. (2011) Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans.
    Am J Pathol. 178 (4): 1662-75.
  4. Gu, Y. et al. (2008) Lymphangiogenesis induced by VEGF-C and VEGF-D promotes metastasis and a poor outcome in breast carcinoma: a retrospective study of 61 cases.
    Clin Exp Metastasis. 25 (7): 717-25.
  5. Ra, S.H. et al. (2007) Recurrent hepatic lymphangiomatosis after orthotopic liver transplantation.
    Liver Transpl. 13 (11): 1593-7.

Further Reading

  1. Matsui, K. et al. (2003) Lymphatic microvessels in the rat remnant kidney model of renal fibrosis: aminopeptidase p and podoplanin are discriminatory markers for endothelial cells of blood and lymphatic vessels.
    J Am Soc Nephrol. 14 (8): 1981-9.
  2. Dadras, S.S. et al. (2003) Tumor lymphangiogenesis: a novel prognostic indicator for cutaneous melanoma metastasis and survival.
    Am J Pathol. 162 (6): 1951-60.
  3. Kato, Y. et al. (2003) Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors.
    J Biol Chem. 278 (51): 51599-605.
  4. Schacht, V. et al. (2003) T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema.
    EMBO J. 22 (14): 3546-56.
  5. Zimmer, G. et al. (1999) Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium.
    Biochem J. 341 ( Pt 2): 277-84.