Placental Alkaline Phosphatase antibody | H17E2
Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used in immunolocalization studies of germ cell tumors and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Placental membrane.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05187
- Entrez Gene
- ALPP
- GO Terms
- GO:0005886 plasma membrane
- GO:0004035 alkaline phosphatase activity
- GO:0016021 integral to membrane
- GO:0046872 metal ion binding
- GO:0009986 cell surface
- GO:0031225 anchored to membrane
- Regulatory
- For research purposes only
Applications of Placental Alkaline Phosphatase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Flow Cytometry | 10ug/ml | ||
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Placenta, seminoma
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Product Specific References
References for Placental Alkaline Phosphatase antibody
-
Travers, P. & Bodmer, W. (1984) Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
Int J Cancer. 33 (5): 633-41. -
Tucker, D.F. et al. (1985) Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
Br J Cancer. 51 (5): 631-9. -
Epenetos, A.A. et al. (1984) An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.
Br J Cancer. 49 (1): 11-5. -
Kalofonos, H.P. et al. (1990) Immunolocalisation of testicular tumours using radiolabelled monoclonal antibody to placental alkaline phosphatase.
J Nucl Med Allied Sci. 34 (4): 294-8. -
Long, M.A. & Rossi, F.M. (2009) Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells.
PLoS One. 4:e5435. -
Carter, C.C. et al. (2010) HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs.
Nat Med. 16: 446-51. -
Wonderlich, E.R. et al. (2011) ADP Ribosylation Factor 1 Activity Is Required To Recruit AP-1 to the Major Histocompatibility Complex Class I (MHC-I) Cytoplasmic Tail and Disrupt MHC-I Trafficking in HIV-1-Infected Primary T Cells.
J Virol. 85: 12216-26. -
Semenov, O.V. et al. (2010) Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
Am J Obstet Gynecol. 202:193.e1-193.e13. -
Göhner, C. et al. (2015) A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids.
Am J Reprod Immunol. 73 (6): 582-8. -
Fisken, J. et al. (1989) Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.
J Clin Pathol. 42 (1): 40-5. -
Orozco AF et al. (2009) Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.
Placenta. 30 (10): 891-7. -
Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
European Polymer Journal. 72: 484-93. -
Dragovic, R.A. et al. (2013) Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
Biol Reprod. 89 (6): 151. -
Fitzgerald, W. et al. (2018) Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors.
Am J Reprod Immunol. 80 (1): e12860.
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