Mouse anti Human placental alkaline phosphatase antibody, clone H17E2
recognizes human placental alkaline phosphatase, also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984
), in seminomas (Lange et al. 1982
)and in ovarian carcinoma (Tholander et al. 1990
Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used in immunolocalization studies of germ cell tumors and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Placental membrane.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
alkaline phosphatase activity
integral to membrane
metal ion binding
anchored to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Placental Alkaline Phosphatase antibody
|Immunohistology - Frozen 1
|Immunohistology - Paraffin
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Placenta, seminoma
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for Placental Alkaline Phosphatase antibody
Travers, P. & Bodmer, W. (1984) Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
Int J Cancer. 33 (5): 633-41.
Tucker, D.F. et al. (1985) Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
Br J Cancer. 51 (5): 631-9.
Epenetos, A.A. et al. (1984) An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.
Br J Cancer. 49 (1): 11-5.
Kalofonos, H.P. et al. (1990) Immunolocalisation of testicular tumours using radiolabelled monoclonal antibody to placental alkaline phosphatase.
J Nucl Med Allied Sci. 34 (4): 294-8.
Long, M.A. & Rossi, F.M. (2009) Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells.
PLoS One. 4:e5435.
Carter, C.C. et al. (2010) HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs.
Nat Med. 16: 446-51.
Wonderlich, E.R. et al. (2011) ADP Ribosylation Factor 1 Activity Is Required To Recruit AP-1 to the Major Histocompatibility Complex Class I (MHC-I) Cytoplasmic Tail and Disrupt MHC-I Trafficking in HIV-1-Infected Primary T Cells.
J Virol. 85: 12216-26.
Semenov, O.V. et al. (2010) Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
Am J Obstet Gynecol. 202:193.e1-193.e13.
Göhner, C. et al. (2015) A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids.
Am J Reprod Immunol. 73 (6): 582-8.
Fisken, J. et al. (1989) Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.
J Clin Pathol. 42 (1): 40-5.
Orozco AF et al. (2009) Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.
Placenta. 30 (10): 891-7.
Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
European Polymer Journal. Jul 2 [Epub ahead of print]
Dragovic, R.A. et al. (2013) Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
Biol Reprod. 89 (6): 151.