Placental Alkaline Phosphatase antibody | H17E2

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Anti Human Placental Alkaline Phosphatase Antibody, clone H17E2 gallery image 1 — **Published customer image:**
Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 (**MCA2091**) used for the detection of PLAP reporter expression on peripheral blood granulocytes by flow cytometrry.
**Image caption:**
Figure 3. Activation of the Cre-reporter gene is less efficient in Z/AP and Z/EG mice than in R26R-EYFP mice. All reporter strains were crossed to the general deleter strain, pCX-NLS-Cre, and activation of each reporter gene was assessed by flow cytometry of peripheral blood leukocytes at 12 weeks of age (A). All reporter strains were also crossed to the myeloid-specific LysM-Cre strain, and activation of each reporter gene was assessed by flow cytometry of peripheral blood granulocytes at 12 weeks of age (B). Representative histograms demonstrating reporter expression in peripheral blood granulocytes are shown in (C–E) for LysM-Cre/R26R-EYFP, LysM-Cre/ZAP and LysM-Cre/Z/EG mice respectively (solid lines). Reporter expression in BL/6 mice is shown in dotted lines.

From: Long MA, Rossi FMV (2009)
Silencing Inhibits Cre-Mediated Recombination of the Z/AP and Z/EG Reporters in Adult Cells.
PLoS ONE 4(5): e5435.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Placental Alkaline Phosphatase

Product Type: Monoclonal Antibody
Clone: H17E2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2091	C* E F			0.2 mg

Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 recognizes human placental alkaline phosphatase, also known as Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982)and in ovarian carcinoma (Tholander et al. 1990).

Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used in immunolocalization studies of germ cell tumors and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Placental membrane.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P05187 Related reagents
Entrez Gene: ALPP Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0004035 alkaline phosphatase activity; GO:0016021 integral to membrane; GO:0046872 metal ion binding; GO:0009986 cell surface; GO:0031225 anchored to membrane
Regulatory: For research purposes only

Applications of Placental Alkaline Phosphatase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry			10ug/ml
Immunohistology - Frozen ¹
Immunohistology - Paraffin

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Histology Positive Control Tissue: Placenta, seminoma

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for Placental Alkaline Phosphatase antibody

Travers, P. & Bodmer, W. (1984) Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
Int J Cancer. 33 (5): 633-41.
Tucker, D.F. et al. (1985) Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
Br J Cancer. 51 (5): 631-9.
Epenetos, A.A. et al. (1984) An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.
Br J Cancer. 49 (1): 11-5.
Kalofonos, H.P. et al. (1990) Immunolocalisation of testicular tumours using radiolabelled monoclonal antibody to placental alkaline phosphatase.
J Nucl Med Allied Sci. 34 (4): 294-8.
Long, M.A. & Rossi, F.M. (2009) Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells.
PLoS One. 4:e5435.
Carter, C.C. et al. (2010) HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs.
Nat Med. 16: 446-51.
Wonderlich, E.R. et al. (2011) ADP Ribosylation Factor 1 Activity Is Required To Recruit AP-1 to the Major Histocompatibility Complex Class I (MHC-I) Cytoplasmic Tail and Disrupt MHC-I Trafficking in HIV-1-Infected Primary T Cells.
J Virol. 85: 12216-26.
Semenov, O.V. et al. (2010) Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
Am J Obstet Gynecol. 202:193.e1-193.e13.
Göhner, C. et al. (2015) A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids.
Am J Reprod Immunol. 73 (6): 582-8.
Fisken, J. et al. (1989) Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.
J Clin Pathol. 42 (1): 40-5.
Orozco AF et al. (2009) Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.
Placenta. 30 (10): 891-7.
Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
European Polymer Journal. Jul 2 [Epub ahead of print]
Dragovic, R.A. et al. (2013) Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
Biol Reprod. 89 (6): 151.

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