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Placental Alkaline Phosphatase antibody | H17E2

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Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 (MCA2091) used for the detection of PLAP reporter expression on peripheral blood granulocytes by flow cytometrry.
Image caption:
Figure 3. Activation of the Cre-reporter gene is less efficient in Z/AP and Z/EG mice than in R26R-EYFP mice. All reporter strains were crossed to the general deleter strain, pCX-NLS-Cre, and activation of each reporter gene was assessed by flow cytometry of peripheral blood leukocytes at 12 weeks of age (A). All reporter strains were also crossed to the myeloid-specific LysM-Cre strain, and activation of each reporter gene was assessed by flow cytometry of peripheral blood granulocytes at 12 weeks of age (B). Representative histograms demonstrating reporter expression in peripheral blood granulocytes are shown in (C–E) for LysM-Cre/R26R-EYFP, LysM-Cre/ZAP and LysM-Cre/Z/EG mice respectively (solid lines). Reporter expression in BL/6 mice is shown in dotted lines.

From: Long MA, Rossi FMV (2009)
Silencing Inhibits Cre-Mediated Recombination of the Z/AP and Z/EG Reporters in Adult Cells.
PLoS ONE 4(5): e5435.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Human Placental Alkaline Phosphatase

Product Type
Monoclonal Antibody
Clone
H17E2
Isotype
IgG1
Specificity
Placental Alkaline Phosphatase

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA2091
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SDS Safety Datasheet SDS
C * E F 0.2 mg loader
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loader
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Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 recognizes human placental alkaline phosphatase, also known as alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982)and in ovarian carcinoma (Tholander et al. 1990).

Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used for immunolocalization of germ cell tumors and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Placental membrane.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 10ug/ml
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Placenta, seminoma

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References for Placental Alkaline Phosphatase antibody

  1. Travers, P. & Bodmer, W. (1984) Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.
    Int J Cancer. 33 (5): 633-41.
  2. Tucker, D.F. et al. (1985) Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.
    Br J Cancer. 51 (5): 631-9.
  3. Epenetos, A.A. et al. (1984) An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.
    Br J Cancer. 49 (1): 11-5.
  4. Kalofonos, H.P. et al. (1990) Immunolocalisation of testicular tumours using radiolabelled monoclonal antibody to placental alkaline phosphatase.
    J Nucl Med Allied Sci. 34 (4): 294-8.
  5. Long, M.A. & Rossi, F.M. (2009) Silencing inhibits Cre-mediated recombination of the Z/AP and Z/EG reporters in adult cells.
    PLoS One. 4:e5435.
  6. Carter, C.C. et al. (2010) HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs.
    Nat Med. 16: 446-51.
  7. Wonderlich, E.R. et al. (2011) ADP Ribosylation Factor 1 Activity Is Required To Recruit AP-1 to the Major Histocompatibility Complex Class I (MHC-I) Cytoplasmic Tail and Disrupt MHC-I Trafficking in HIV-1-Infected Primary T Cells.
    J Virol. 85: 12216-26.
  8. Semenov, O.V. et al. (2010) Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Am J Obstet Gynecol. 202:193.e1-193.e13.
    9. View The Latest Product References
  9. Göhner, C. et al. (2015) A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids.
    Am J Reprod Immunol. 73 (6): 582-8.
  10. Fisken, J. et al. (1989) Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.
    J Clin Pathol. 42 (1): 40-5.
  11. Orozco AF et al. (2009) Placental release of distinct DNA-associated micro-particles into maternal circulation: reflective of gestation time and preeclampsia.
    Placenta. 30 (10): 891-7.
  12. Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
    European Polymer Journal. 72: 484-93.
  13. Dragovic, R.A. et al. (2013) Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women.
    Biol Reprod. 89 (6): 151.
  14. Fitzgerald, W. et al. (2018) Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors.
    Am J Reprod Immunol. 80 (1): e12860.

Flow Cytometry

Synonyms
PLAP
RRID
AB_2226283
UniProt
P05187
Entrez Gene
ALPP
GO Terms
GO:0005886 plasma membrane
GO:0004035 alkaline phosphatase activity
GO:0016021 integral to membrane
GO:0046872 metal ion binding
GO:0009986 cell surface
GO:0031225 anchored to membrane
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