p21/WAF1 antibody | WA-1

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Anti Human p21/WAF1 Antibody, clone WA-1 gallery image 1 — p21/WAF1 transfected cell lysate probed with Mouse anti Human p21/WAF1 antibody, clone WA-1 (**MCA2325**).

Mouse anti Human p21/WAF1

Product Type: Monoclonal Antibody
Clone: WA-1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2325	IP WB			0.1 mg

Mouse anti Human p21/WAF1 antibody, clone WA-1 recognizes human Cyclin-dependent kinase inhibitor 1, also konwn as CDK-interacting protein 1, cyclin-dependent kinase inhibitor p21/WAF1, p21, WAF1, CIP-1, Melanoma differentiation-associated protein 6 or MDA-6. The CDK inhibitor p21 is an important cell cycle regulatory protein which inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli.

Mouse anti Human p21/WAF1 antibody, clone WA-1 detects a band of approximately 21 kDa in MCF-7 cell lysates following UV radiation by western blotting.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Ig fraction prepared by ammonium sulphate precipitation

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Human p21 protein.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P38936 Related reagents
Entrez Gene: CDKN1A Related reagents
GO Terms: GO:0000080 G1 phase of mitotic cell cycle; GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0000086 G2/M transition of mitotic cell cycle; GO:0000307 cyclin-dependent protein kinase holoenzyme complex; GO:0005829 cytosol; GO:0004861 cyclin-dependent protein kinase inhibitor activity; GO:0046872 metal ion binding; GO:0005654 nucleoplasm; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0007050 cell cycle arrest; GO:0007265 Ras protein signal transduction; GO:0008285 negative regulation of cell proliferation; GO:0008629 induction of apoptosis by intracellular signals; GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process; GO:0030308 negative regulation of cell growth; GO:0031668 cellular response to extracellular stimulus; GO:0048146 positive regulation of fibroblast proliferation; GO:0070557 PCNA-p21 complex; GO:0071479 cellular response to ionizing radiation; GO:0090400 stress-induced premature senescence
Regulatory: For research purposes only

Applications of p21/WAF1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/100	1/1000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Western Blotting

Product Specific References

References for p21/WAF1 antibody

Horký, M. et al. (1997) Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy.
Physiol Res. 46 (3): 233-5.
Kotala, V. et al. (2001) Potent induction of wild-type p53-dependent transcription in tumour cells by a synthetic inhibitor of cyclin-dependent kinases.
Cell Mol Life Sci. 58 (9): 1333-9.
Kolár, Z. et al. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer.
Mol Pathol. 53 (1): 15-8.
Khanna, S.J. et al. (2001) v-Abl protein-tyrosine kinase up-regulates p21WAF-1 in cell cycle arrested and proliferating myeloid cells.
J Biol Chem. 276 (14): 11143-50.
Scott, M.T. et al. (2002) PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
EMBO J. 21 (24): 6771-80.
Blaydes, J.P. et al. (2001) Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription.
J Biol Chem. 276 (7): 4699-708.

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