p21/WAF1 antibody | WA-1
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WB ResetMouse anti Human p21/WAF1
- Product Type
- Monoclonal Antibody
- Clone
- WA-1
- Isotype
- IgG1
- Specificity
- p21/WAF1
Antibody Quality and Performance Guarantee
| Mouse anti Human p21/WAF1 antibody, clone WA-1 recognizes human Cyclin-dependent kinase inhibitor 1, also konwn as CDK-interacting protein 1, cyclin-dependent kinase inhibitor p21/WAF1, p21, WAF1, CIP-1, Melanoma differentiation-associated protein 6 or MDA-6. The CDK inhibitor p21 is an important cell cycle regulatory protein which inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli. Mouse anti Human p21/WAF1 antibody, clone WA-1 detects a band of approximately 21 kDa in MCF-7 cell lysates following UV radiation by western blotting. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human p21 protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for p21/WAF1 antibody
-
Horký, M. et al. (1997) Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy.
Physiol Res. 46 (3): 233-5. -
Kolár, Z. et al. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer.
Mol Pathol. 53 (1): 15-8. -
Khanna, S.J. et al. (2001) v-Abl protein-tyrosine kinase up-regulates p21WAF-1 in cell cycle arrested and proliferating myeloid cells.
J Biol Chem. 276 (14): 11143-50. -
Scott, M.T. et al. (2002) PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
EMBO J. 21 (24): 6771-80. -
Blaydes, J.P. et al. (2001) Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription.
J Biol Chem. 276 (7): 4699-708.
- RRID
- AB_2077672
- UniProt
- P38936
- Entrez Gene
- CDKN1A
- GO Terms
- GO:0000080 G1 phase of mitotic cell cycle
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000307 cyclin-dependent protein kinase holoenzyme complex
- GO:0005829 cytosol
- GO:0004861 cyclin-dependent protein kinase inhibitor activity
- GO:0046872 metal ion binding
- GO:0005654 nucleoplasm
- View More GO Terms
- GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
- GO:0007050 cell cycle arrest
- GO:0007265 Ras protein signal transduction
- GO:0008285 negative regulation of cell proliferation
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
- GO:0030308 negative regulation of cell growth
- GO:0031668 cellular response to extracellular stimulus
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0070557 PCNA-p21 complex
- GO:0071479 cellular response to ionizing radiation
- GO:0090400 stress-induced premature senescence
MCA2325
152851
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