p21/WAF1 antibody | WA-1

Mouse anti Human p21/WAF1

Product Type
Monoclonal Antibody
Clone
WA-1
Isotype
IgG1
Specificity
p21/WAF1

Product Code Applications Pack Size List Price Your Price Qty
MCA2325
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human p21/WAF1 antibody, clone WA-1 recognizes human Cyclin-dependent kinase inhibitor 1, also konwn as CDK-interacting protein 1, cyclin-dependent kinase inhibitor p21/WAF1, p21, WAF1, CIP-1, Melanoma differentiation-associated protein 6 or MDA-6. The CDK inhibitor p21 is an important cell cycle regulatory protein which inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli.

Mouse anti Human p21/WAF1 antibody, clone WA-1 detects a band of approximately 21 kDa in MCF-7 cell lysates following UV radiation by western blotting.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human p21 protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/100 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for p21/WAF1 antibody

  1. Horký, M. et al. (1997) Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy.
    Physiol Res. 46 (3): 233-5.
  2. Kolár, Z. et al. (2000) Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer.
    Mol Pathol. 53 (1): 15-8.
  3. Khanna, S.J. et al. (2001) v-Abl protein-tyrosine kinase up-regulates p21WAF-1 in cell cycle arrested and proliferating myeloid cells.
    J Biol Chem. 276 (14): 11143-50.
  4. Scott, M.T. et al. (2002) PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
    EMBO J. 21 (24): 6771-80.
  5. Blaydes, J.P. et al. (2001) Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription.
    J Biol Chem. 276 (7): 4699-708.

Western Blotting

RRID
AB_2077672
UniProt
P38936
Entrez Gene
CDKN1A
GO Terms
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000307 cyclin-dependent protein kinase holoenzyme complex
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0046872 metal ion binding
GO:0005654 nucleoplasm
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007050 cell cycle arrest
GO:0007265 Ras protein signal transduction
GO:0008285 negative regulation of cell proliferation
GO:0008629 induction of apoptosis by intracellular signals
GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
GO:0030308 negative regulation of cell growth
GO:0031668 cellular response to extracellular stimulus
GO:0048146 positive regulation of fibroblast proliferation
GO:0070557 PCNA-p21 complex
GO:0071479 cellular response to ionizing radiation
GO:0090400 stress-induced premature senescence
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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