p19 antibody | DCS100.1

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Mouse anti Human p19

Product Type
Monoclonal Antibody
Clone
DCS100.1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1991 C IP* WB 0.1 mg
Mouse anti Human p19 antibody, clone DCS100.1 recognizes human p19-INK4d, also known as Cyclin-dependent kinase 4 inhibitor D. p19-INK4d is a 166 amino acis ~19 kDa tumor suppressor protein, a specific inhibitor of cdk4/cdk6. p19ink4d is periodically expressed during the cell cycle. Maximum induction of p19ink4d occurs as cells enter S phase.

Mouse anti Human p19 antibody, clone DCS100.1 co-precipitates cdk4/cdk6 in immunoprecipitation studies.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G and A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.08%Sodium Azide
Immunogen
Full length recombinant p19-His tagged.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P55273 Related reagents
Entrez Gene
CDKN2D Related reagents
GO Terms
GO:0000079 regulation of cyclin-dependent protein kinase activity
GO:0000080 G1 phase of mitotic cell cycle
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000731 DNA synthesis involved in DNA repair
GO:0005829 cytosol
GO:0004861 cyclin-dependent protein kinase inhibitor activity
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007050 cell cycle arrest
GO:0048102 autophagic cell death
GO:0008285 negative regulation of cell proliferation
GO:0009411 response to UV
GO:0019901 protein kinase binding
GO:0030308 negative regulation of cell growth
GO:0032526 response to retinoic acid
GO:0033280 response to vitamin D
GO:0043154 negative regulation of caspase activity
Regulatory
For research purposes only

Applications of p19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation 1
Western Blotting
  1. 1Special conditions apply. - See [*].
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Spleen

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for p19 antibody

  1. Hirai, H. et al. (1995) Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6.
    Mol Cell Biol. 15 (5): 2672-81.