Mouse anti Human osteocalcin antibody, clone 2D4 recognizes human osteocalcin, also known as bone gla protein or Gamma-carboxyglutamic acid-containing protein. Osteocalcin is a 49 amino acid ~5 kDa calcium-binding bone protein comprising ~1-2% total bone protein mass. Osteocalcin also has an additional 28 amino acid propeptide region along with a 23 amino acid signal peptide region.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 52-100 of human osteocalcin.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
calcium ion binding
structural constituent of bone
regulation of bone mineralization
regulation of bone resorption
regulation of osteoclast differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Osteocalcin antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Osteocalcin antibody
Kover, K. et al. (2011) Bone marrow cavity: a supportive environment for islet engraftment.
Islets. 3: 93-101.
Oliveira, M.B.et al. (2016) Autonomous osteogenic differentiation of hASCs encapsulated in methacrylated gellan-gum hydrogels
Acta Biomaterialia. May 24[Epub ahead of print]
Mizoshiri, N. et al. (2015) Transduction of Oct6 or Oct9 gene concomitant with Myc family gene induced osteoblast-like phenotypic conversion in normal human fibroblasts.
Biochem Biophys Res Commun. 467 (4): 1110-6.