OCLN antibody | 1G7
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WB IF ResetMouse anti Human OCLN:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 1G7
- Isotype
- IgG1
- Specificity
- OCLN
Antibody Quality and 
Unrivaled 
| Mouse anti Human occludin antibody, clone 1G7 recognizes human occludin, a 522 amino acid ~60 kDa protein involved in the regulation of tight junctions (McCarthy et al. 1996). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 423-523 of human OCLN.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
0.1 | 10 ug/ml |
| Western Blotting | |
0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for OCLN antibody
-
Wang, J. et al. (2014) Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection.
J Virol Methods. 202: 87-94.
- RRID
- AB_2156328
- UniProt
- Q16625
- Entrez Gene
- OCLN
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005198 structural molecule activity
- GO:0005923 tight junction
- GO:0006461 protein complex assembly
- GO:0006921 cellular component disassembly involved in apoptosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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