OCLN antibody | 1G7

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Mouse anti Human OCLN:Preservative Free

Product Type
Monoclonal Antibody
Clone
1G7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA3308Z IF WB 0.1 mg
Mouse anti Human occludin antibody, clone 1G7 recognizes human occludin, a 522 amino acid ~60 kDa protein involved in the regulation of tight junctions (McCarthy et al. 1996).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 423-523 of human OCLN.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q16625 Related reagents
Entrez Gene
OCLN Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005198 structural molecule activity
GO:0005923 tight junction
GO:0006461 protein complex assembly
GO:0006921 cellular component disassembly involved in apoptosis
Regulatory
For research purposes only

Applications of OCLN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for OCLN antibody

  1. Wang, J. et al. (2014) Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection.
    J Virol Methods. 202: 87-94.