Mouse anti Human Nuclear Antigen, clone 1415-1, preferentially reacts with histone H1, a nuclear protein necessary for the condensation of nucleosome chains into higher order structures. This clone stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Nuclear Antigen antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin
|Western Blotting 1
- 1Clone 1415-1 recognizes a primary band between 30 and 32kDa under non-reducing conditions. Other bands may also be present
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Tonsil
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Secondary Antibodies Available