Nuclear Antigen antibody | 1415-1 (21NC85)
Mouse anti Human nuclear antigen, clone 1415-1, preferentially reacts with histone H1, a nuclear protein necessary for the condensation of nucleosome chains into higher order structures. Mouse anti Human nuclear antigen, clone 1415-1 stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by protein A and ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Native
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Nuclear Antigen antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | 1/20 | 1/30 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | 1/50 | ||
| Western Blotting 1 |
- 1Clone 1415-1 recognizes a primary band between 30 and 32kDa under non-reducing conditions. Other bands may also be present
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Tonsil
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Secondary Antibodies Available
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