Myeloperoxidase antibody | 4A4
Mouse anti Human myeloperoxidase antibody, clone 4A4 (0400-0002) used as a capture reagent for the evaluation of MPO-DNA adducts by ELISA.
Image caption:
NETs and NETosis markers by clinical group. a Myeloperoxidase (MPO)-DNA, b peroxidase activity, and c cell-free DNA (cf-DNA) are compared in ventilator associated pneumonia (VAP) with and without acute respiratory distress syndrome (ARDS). MPO-DNA is higher in ARDS patients with VAP. P values are for Kruskal-Wallis (nonparametric analysis of variance (ANOVA) with post-test Dunn's to account for multiple pairwise tests). Error bars show the median and interquartile range. ***p<0.0001, *p<0.05
From: Mikacenic C, Moore R, Dmyterko V, West TE, Altemeier WA, Liles WC, Lood C.
Neutrophil extracellular traps (NETs) are increased in the alveolar spaces of patients with ventilator-associated pneumonia.
Crit Care. 2018 Dec 27;22(1):358.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Myeloperoxidase antibody, clone 4A4 (0400-0002) used as a coating antibody in an ELISA designed to measure MPO-DNA complexes in human sera.
Image caption:
Distribution of MPO-DNA complex levels among age categories.
Data are presented as median and 25th-75th percentiles. Category 55–65 years, (n = 2286) MPO-DNA complex 56 mAU/mL (45–93), category 65–75 years (n = 2467) MPO-DNA complex 53 mAU/mL (41–83), category >75 years (n = 1674) MPO-DNA complex 52 AU/mL (41–81). Differences between age categories were analyzed using the Kruskall–Wallis test with post-hoc analysis. *p<0.01.
From: Donkel SJ, Wolters FJ, Ikram MA, de Maat MPM (2021)
Circulating Myeloperoxidase (MPO)-DNA complexes as marker for Neutrophil Extracellular Traps (NETs) levels and the association with cardiovascular risk factors in the general population.
PLoS ONE 16(8): e0253698.
doi:10.1371/journal.pone.0253698
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Myeloperoxidase antibody, clone 4A4 (0400-0002) use as a capture reagent in an ELISA to measure MPO-DNA complexes in human plasma.
Image caption:
: Plasma levels of H3Cit-DNA and MPO-DNA in healthy controls and patients with different Child-Pugh scores. a) Patients with Child-Pugh B and C (CPB-C) had significantly elevated H3Cit-DNA levels as compared to healthy controls and patients with Child-Pugh A (CPA). The difference between Child-Pugh A and healthy controls was not significant. b) Patients with Child-Pugh B and C (CPB-C) had significantly elevated MPO-DNA levels as compared to healthy controls and patients with Child-Pugh A (CPA). The difference between Child-Pugh A and healthy controls was not significant.
From: Zenlander R, Havervall S, Magnusson M, Engstrand J, Ågren A, Thålin C, Stål P.
Neutrophil extracellular traps in patients with liver cirrhosis and hepatocellular carcinoma.
Sci Rep. 2021 Sep 9;11(1):18025
doi: 10.1038/s41598-021-97233-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Myeloperoxidase antibody, clone 4A4 (0400-0002) used as a capture reagent in a sandwich ELISAA for the determination of MPO-DNA complexes in serum samples.
Image caption:
TCC levels in quartiles related to levels of NET markers. Quartiles (Q) of TCC related to markers of NETs. Solid line indicates the threshold for dichotomizing levels. p and p1 refers to the Kruskal-Wallis test across quartiles; p2 refers to the Mann–Whitney U test. TCC: terminal complement complex; AU: arbitrary units.
From: Kluge KE, Langseth MS, Opstad TB, Pettersen AÅ, Arnesen H, Tønnessen T, Seljeflot I, Helseth R.
Complement Activation in Association with Markers of Neutrophil Extracellular Traps and Acute Myocardial Infarction in Stable Coronary Artery Disease.
Mediators Inflamm. 2020 Mar 26;2020:5080743.
doi: 10.1155/2020/5080743.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Myeloperoxidase antibody, clone 4A4 (0400-0002) used as a capture reagent for the determination of MPO-DNA complexes in venous serum samples by sandwich ELISA.
Image caption
Number of primary composite endpoints and deaths according to above- or below-median levels of dsDNA (blue), MPO-DNA (green), and CitH3 (red). The p-values refer to Chi-square tests. Figure created using software from IBM Corp. Released 2017. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM
Insert image caption
From: Langseth MS, Helseth R, Ritschel V, Hansen CH, Andersen GØ, Eritsland J, Halvorsen S, Fagerland MW, Solheim S, Arnesen H, Seljeflot I, Opstad TB.
Double-Stranded DNA and NETs Components in Relation to Clinical Outcome After ST-Elevation Myocardial Infarction.
Sci Rep. 2020 Mar 19;10(1):5007.
doi: 10.1038/s41598-020-61971-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Myeloperoxidase antibody, clone 4A4 (0400-0002) used as a capture reagent for the detection of MPO-DNA adjuncts by ELISA.
Image caption:
Enhanced NETs release in sepsis-associated ALI patients and mouse models. (A) Serum cell-free DNA and (B) MPO-DNA complexes were detected in healthy controls, sepsis patients, and patients with sepsis-induced ARDS. HC (n=20), Sepsis (n=24), Sepsis ARDS (n=22). (C) A positive correlation was found between serum cell-free DNA and (D) MPO-DNA complexes concerning the IL-8 level in healthy controls and patients with sepsis ARDS. HC, Sepsis ARDS; dot in box means Sepsis ARDS patients; *P<0.05, **P<0.01 (two-way ANOVA with Tukey's correction).
From: Zhang H, Liu J, Zhou Y, Qu M, Wang Y, Guo K, Shen R, Sun Z, Cata JP, Yang S, Chen W, Miao C.
Neutrophil extracellular traps mediate m6A modification and regulates sepsis-associated acute lung injury by activating ferroptosis in alveolar epithelial cells.
Int J Biol Sci. 2022 May 9;18(8):3337-3357.
doi: 10.7150/ijbs.69141.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human myeloperoxidase antibody, clone 4A4 (0400-0002) used as a capture reagent in an ELISA of MPO-DNA levels in serum samples.
Image caption:
ELISA analysis for serum levels of MPO-DNA (serum marker for NETs) in healthy controls, HCC patients and HBV-HCC patients. *P < 0.05, **P < 0.01, ***P < 0.001.
From: Zhan X, Wu R, Kong XH, You Y, He K, Sun XY, Huang Y, Chen WX, Duan L.
Elevated neutrophil extracellular traps by HBV-mediated S100A9-TLR4/RAGE-ROS cascade facilitate the growth and metastasis of hepatocellular carcinoma.
Cancer Commun (Lond). 2022 Nov 8. Epub ahead of print.
doi: 10.1002/cac2.12388.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human myeloperoxidase antibody, clone 4A4 (0400-0002) used as a capture antibody in an ELISA for the evaluation of MPO-DNA complexes in serum samples.
Image caption:
NETs formation is enhanced in CRC, especially in Fn−high CRC
G ELISA analysis for MPO-DNA in the serum from HCs (n = 56), Fn−low (n = 48) and Fn−high (n = 47) CRC patients. H Correlation between serum MPO-DNA levels and -ΔCt of Fn quantification in the clinical fecal samples from CRC patients. White arrow: Fn; red arrow: CD66b.+ neutrophil. White scale bars: 100 μm; Black scale bars: 50 μm. *p <0.05, **p <0.01, ***p <0.001
From: Kong X, Zhang Y, Xiang L, You Y, Duan Y, Zhao Y, Li S, Wu R, Zhang J, Zhou L, Duan L.
Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression.
J Exp Clin Cancer Res. 2023 Sep 9;42(1):236.
doi: 10.1186/s13046-023-02817-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Myeloperoxidase
- Product Type
- Monoclonal Antibody
- Clone
- 4A4
- Isotype
- IgG2b
- Specificity
- Myeloperoxidase
Antibody Quality and Performance Guarantee
| Mouse anti Human Myeloperoxidase antibody, clone 4A4 recognizes myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (~55 kDa) and two light chains (~15 kDa), the heavy chains being linked by a disulphide bond. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human myeloperoxidase.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Myeloperoxidase antibody
-
Demoruelle, M.K. et al. (2017) Anti-Citrullinated Protein Antibodies Are Associated With Neutrophil Extracellular Traps in the Sputum in Relatives of Rheumatoid Arthritis Patients.
Arthritis Rheumatol. 69 (6): 1165-75. -
Chatfield, S.M. et al. (2018) Monosodium Urate Crystals Generate Nuclease-Resistant Neutrophil Extracellular Traps via a Distinct Molecular Pathway.
J Immunol. 200 (5): 1802-16. -
Mikacenic, C. et al. (2018) Neutrophil extracellular traps (NETs) are increased in the alveolar spaces of patients with ventilator-associated pneumonia.
Crit Care. 22 (1): 358. -
Maugeri, N. et al. (2018) Platelet microparticles sustain autophagy-associated activation of neutrophils in systemic sclerosis.
Sci Transl Med. 10 (451): eaao3089. -
Demoruelle, M.K. et al. (2018) Antibody Responses to Citrullinated and Noncitrullinated Antigens in the Sputum of Subjects With Rheumatoid Arthritis and Subjects at Risk for Development of Rheumatoid Arthritis.
Arthritis Rheumatol. 70 (4): 516-27. -
Guo, L. et al. (2019) A high-risk luminal A dominant breast cancer subtype with increased mobility.
Breast Cancer Res Treat. 175 (2): 459-72. -
Helseth, R. et al. (2019) Neutrophil Extracellular Trap Components Associate with Infarct Size, Ventricular Function, and Clinical Outcome in STEMI.
Mediators of Inflammation. 2019: 1-10. -
Kluge, K.E. et al. (2020) Complement Activation in Association with Markers of Neutrophil Extracellular Traps and Acute Myocardial Infarction in Stable Coronary Artery Disease.
Mediators Inflamm. 2020: 5080743.
View The Latest Product References
-
Langseth, M.S. et al. (2020) Double-Stranded DNA and NETs Components in Relation to Clinical Outcome After ST-Elevation Myocardial Infarction.
Sci Rep. 10 (1): 5007. -
Donkel, S.J. et al. (2021) Circulating Myeloperoxidase (MPO)-DNA complexes as marker for Neutrophil Extracellular Traps (NETs) levels and the association with cardiovascular risk factors in the general population.
PLoS One. 16 (8): e0253698. -
Zenlander, R. et al. (2021) Neutrophil extracellular traps in patients with liver cirrhosis and hepatocellular carcinoma.
Sci Rep. 11 (1): 18025. -
Zapponi, K.C.S. et al. (2021) Neutrophil activation and circulating neutrophil extracellular traps are increased in venous thromboembolism patients for at least one year after the clinical event.
J Thromb Thrombolysis. 53 (1): 30-42. -
Wang, W.W. et al. (2021) Lipopolysaccharides increase the risk of colorectal cancer recurrence and metastasis due to the induction of neutrophil extracellular traps after curative resection.
J Cancer Res Clin Oncol. 147 (9): 2609-2619. -
Casey, K.A. et al. (2021) Modulation of Cardiometabolic Disease Markers by Type I Interferon Inhibition in Systemic Lupus Erythematosus.
Arthritis Rheumatol. 73 (3): 459-71. -
Mazetto, B.M. et al. (2022) Association between neutrophil extracellular traps (NETs) and thrombosis in antiphospholipid syndrome.
Thromb Res. 214: 132-7. -
Zhang, H. et al. (2022) Neutrophil extracellular traps mediate m6A modification and regulates sepsis-associated acute lung injury by activating ferroptosis in alveolar epithelial cells.
Int J Biol Sci. 18 (8): 3337-3357. -
Zhan, X. et al. (2022) Elevated neutrophil extracellular traps by HBV-mediated S100A9-TLR4/RAGE-ROS cascade facilitate the growth and metastasis of hepatocellular carcinoma.
Cancer Commun (Lond). Nov 08 [Epub ahead of print]. -
Takeuchi, S. et al. (2022) Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA Vasculitis.
Pathobiology. 89 (1): 23-8. -
Manfredi, A.A. et al. (2022) Platelet Phagocytosis via P-selectin Glycoprotein Ligand 1 and Accumulation of Microparticles in Systemic Sclerosis.
Arthritis Rheumatol. 74 (2): 318-28. -
Liu, L. et al. (2022) Promising Neutrophil-Associated Biomarkers in Lung Diseases of Patients with Antisynthetase Syndrome and Dermatomyositis.
J Immunol Res. 2022: 1886083. -
Tilly, M.J. et al. (2022) Immunothrombosis and new-onset atrial fibrillation in the general population: the Rotterdam Study.
Clin Res Cardiol. 111 (1): 96-104. -
Okamoto, M. et al. (2023) Neutrophil Extracellular Traps Promote Metastases of Colorectal Cancers through Activation of ERK Signaling by Releasing Neutrophil Elastase.
Int J Mol Sci. 24 (2): 1118. -
Liu, C. et al. (2023) An Inhalable Hybrid Biomimetic Nanoplatform for Sequential Drug Release and Remodeling Lung Immune Homeostasis in Acute Lung Injury Treatment.
ACS Nano. 17 (12): 11626-44. -
Kong, X. et al. (2023) Fusobacterium nucleatum-triggered neutrophil extracellular traps facilitate colorectal carcinoma progression.
J Exp Clin Cancer Res. 42 (1): 236. -
Zhang, H. et al. (2022) Intraoperative lidocaine infusion in patients undergoing pancreatectomy for pancreatic cancer: a mechanistic, multicentre randomised clinical trial.
Br J Anaesth. 129 (2): 244-53. -
Garcia, G. et al. (2024) Impaired balance between neutrophil extracellular trap formation and degradation by DNases in COVID-19 disease.
J Transl Med. 22 (1): 246. -
Zhu, D. et al. (2024) Neutrophil activation biomarker pentraxin 3 for diagnosis and monitoring of macrophage activation syndrome occurrence in adult-onset Still's disease.
J Autoimmun. 144: 103182. -
Jütte, B.B. et al. (2021) Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiency.
iScience. 24 (8): 102833.
- RRID
- AB_617350
- UniProt
- P05164
- Entrez Gene
- MPO
- GO Terms
- GO:0003682 chromatin binding
- GO:0006952 defense response
- GO:0005764 lysosome
- GO:0004601 peroxidase activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0030141 stored secretory granule
- GO:0055114 oxidation-reduction process
- GO:0006916 anti-apoptosis
- View More GO Terms
- GO:0008201 heparin binding
- GO:0020037 heme binding
- GO:0034374 low-density lipoprotein particle remodeling
- GO:0042744 hydrogen peroxide catabolic process
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