Myeloperoxidase antibody | 4A4

Mouse anti Human Myeloperoxidase

Product Type
Monoclonal Antibody
Clone
4A4
Isotype
IgG2b
Specificity
Myeloperoxidase

Product Code Applications Pack Size List Price Your Price Qty
0400-0002
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human Myeloperoxidase antibody, clone 4A4 recognizes myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (~55 kDa) and two light chains (~15 kDa), the heavy chains being linked by a disulphide bond.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human myeloperoxidase.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Myeloperoxidase antibody

  1. Chatfield, S.M. et al. (2018) Monosodium Urate Crystals Generate Nuclease-Resistant Neutrophil Extracellular Traps via a Distinct Molecular Pathway.
    J Immunol. 200 (5): 1802-16.
  2. Demoruelle, M.K. et al. (2017) Anti-Citrullinated Protein Antibodies Are Associated With Neutrophil Extracellular Traps in the Sputum in Relatives of Rheumatoid Arthritis Patients.
    Arthritis Rheumatol. 69 (6): 1165-75.
  3. Demoruelle, M.K. et al. (2018) Antibody Responses to Citrullinated and Noncitrullinated Antigens in the Sputum of Subjects With Rheumatoid Arthritis and Subjects at Risk for Development of Rheumatoid Arthritis.
    Arthritis Rheumatol. 70 (4): 516-27.
  4. Mikacenic, C. et al. (2018) Neutrophil extracellular traps (NETs) are increased in the alveolar spaces of patients with ventilator-associated pneumonia.
    Crit Care. 22 (1): 358.
  5. Guo, L. et al. (2019) A high-risk luminal A dominant breast cancer subtype with increased mobility.
    Breast Cancer Res Treat. 175 (2): 459-72.
  6. Helseth, R. et al. (2019) Neutrophil Extracellular Trap Components Associate with Infarct Size, Ventricular Function, and Clinical Outcome in STEMI.
    Mediators of Inflammation. 2019: 1-10.
  7. Donkel, S.J. et al. (2021) Circulating Myeloperoxidase (MPO)-DNA complexes as marker for Neutrophil Extracellular Traps (NETs) levels and the association with cardiovascular risk factors in the general population.
    PLoS One. 16 (8): e0253698.
  8. Zenlander, R. et al. (2021) Neutrophil extracellular traps in patients with liver cirrhosis and hepatocellular carcinoma.
    Sci Rep. 11 (1): 18025.
  9. View The Latest Product References
  10. Zapponi, K.C.S. et al. (2021) Neutrophil activation and circulating neutrophil extracellular traps are increased in venous thromboembolism patients for at least one year after the clinical event.
    J Thromb Thrombolysis. Aug 27 [Epub ahead of print].
  11. Kluge, K.E. et al. (2020) Complement Activation in Association with Markers of Neutrophil Extracellular Traps and Acute Myocardial Infarction in Stable Coronary Artery Disease.
    Mediators Inflamm. 2020: 5080743.
  12. Langseth, M.S. et al. (2020) Double-Stranded DNA and NETs Components in Relation to Clinical Outcome After ST-Elevation Myocardial Infarction.
    Sci Rep. 10 (1): 5007.
  13. Tilly, M.J. et al. (2022) Immunothrombosis and new-onset atrial fibrillation in the general population: the Rotterdam Study.
    Clin Res Cardiol. 111 (1): 96-104.
  14. Takeuchi, S. et al. (2022) Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA Vasculitis.
    Pathobiology. 89 (1): 23-8.

RRID
AB_617350
UniProt
P05164
Entrez Gene
MPO
GO Terms
GO:0003682 chromatin binding
GO:0006952 defense response
GO:0005764 lysosome
GO:0004601 peroxidase activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0030141 stored secretory granule
GO:0055114 oxidation-reduction process
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0020037 heme binding
GO:0034374 low-density lipoprotein particle remodeling
GO:0042744 hydrogen peroxide catabolic process
View more products with MYELOPEROXIDASE specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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