MMP-1 antibody | F08-2F1

Mouse anti Human MMP-1

Product Type
Monoclonal Antibody
Clone
F08-2F1
Isotype
IgG1
Specificity
MMP-1

Product Code Applications Pack Size List Price Your Price Qty
MCA6254GA
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
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Mouse anti Human MMP-1 antibody, clone F08-2F1, recognizes matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase. MMP-1 is a 45 kDa protein in its active form and is a member of the metzincins superfamily of proteases. It is produced by various cells and tissues including the endothelium, fibroblasts, smooth muscle cells, vascular adventitia and platelets (Cui et al. 2017).

MMP-1 is largely expressed by connective and vascular tissues and cells due to its roles in extracellular matrix remodeling. It is synthesized as a pre-proenzyme before being processed to remove the signal peptide forming the pro-MMP-1. This is then further cleaved by proteolytic enzymes to create the active form. Like other MMP’s, MMP-1 plays a major role in tissue remodeling through the promotion of extracellular matrix (ECM) protein turnover. Specifically MMP-1 degrades the collagen proteins type I, II, III, VII, VIII, X and gelatin. It is also able to target other ECM substrates including aggrecan, nidogen and perlecan (Cui et al. 2017).

MMP1 expression levels are low in most cells under physiological conditions, but are upregulated during inflammatory conditions and autoimmune diseases (Mittal et al. 2016). For example a single nucleotide polymorphism of MMP-1 at the promoter region has been associated with an increased risk of sudden deafness linked to circulatory disturbance and inflammation (Nam et al. 2011).

The purified Mouse anti Human MMP-1 antibody, clone F08-2F1 (MCA6254GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human MMP-1 antibody, clone F09-3C7 (MCA6255B) as the detection antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human partial length of MMP-1 (Phe100-Thr274)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Detection Antibody: MCA6255B
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human MMP-1:Biotin MCA6255B E 0.1 mg loader
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loader
Description Mouse anti Human MMP-1:Biotin

Synonyms
Interstitial Collagenase
UniProt
P03956
Entrez Gene
MMP1
GO Terms
GO:0005509 calcium ion binding
GO:0007596 blood coagulation
GO:0004222 metalloendopeptidase activity
GO:0005578 proteinaceous extracellular matrix
GO:0006508 proteolysis
GO:0008270 zinc ion binding
GO:0030574 collagen catabolic process
GO:0044419 interspecies interaction between organisms
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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