MICA/Micb antibody | 6D4
Mouse anti Human MICA/Micb antibody, clone 6D4 recognizes non-classical MHC class I chain A (MICA) and non-classical MHC class I chain MICB (MICB).
MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells.
MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection.
Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells (Jinushi et al. 2003).
MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells.
MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection.
Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells (Jinushi et al. 2003).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- MICA transfected C1R cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised RBF/DnJ mice were fused with cells of the P3 mouse myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of MICA/Micb antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Functional Assays | |||
| Immunohistology - Frozen | 1/100 | ||
| Immunoprecipitation | |||
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | 1/100 | ||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Low Endotoxin | MCA929EL | F | 0.5 mg |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for MICA/Micb antibody
-
Groh, V. et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.
Science. 279 (5357): 1737-40. -
Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Science. 285 (5428): 727-9. -
Groh, V. et al. (1999) Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB.
Proc Natl Acad Sci U S A. 96 (12): 6879-84. -
Groh, V. et al. (2001) Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells.
Nat Immunol. 2 (3): 255-60. -
Das, H. et al. (2004) Mechanisms of Vdelta1 gammadelta T cell activation by microbial components.
J Immunol. 172 (11): 6578-86. -
Jinushi, M. et al. (2003) Critical role of MHC class I-related chain A and B expression on IFN-alpha-stimulated dendritic cells in NK cell activation: impairment in chronic hepatitis C virus infection.
J Immunol. 170 (3): 1249-56. -
Groh, V. et al. (2003) Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
Proc Natl Acad Sci U S A. 100: 9452-7. -
Jinushi, M. et al. (2003) Autocrine/paracrine IL-15 that is required for type I IFN-mediated dendritic cell expression of MHC class I-related chain A and B is impaired in hepatitis C virus infection.
J Immunol. 171: 5423-9. -
Park, E.J. et al. (2003) Clonal expansion of double-positive intraepithelial lymphocytes by MHC class I-related chain A expressed in mouse small intestinal epithelium.
J Immunol. 171: 4131-9. -
Wu, J. et al. (2003) Intracellular retention of the MHC class I-related chain B ligand of NKG2D by the human cytomegalovirus UL16 glycoprotein.
J Immunol. 170: 4196-200. -
Xu, X. et al. (2006) Clinicopathological significance of major histocompatibility complex class I-related chain a and B expression in thyroid cancer.
J Clin Endocrinol Metab. 91: 2704-12. -
Shi, J. et al. (2008) Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
Blood. 111: 1309-17.
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