MCM5 antibody | CRCT5.1
Immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy (Murphy et al. 2004).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Bacterially expressed human Mcm5.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised CBI/CFI mice were fused with cells of the mouse SP2 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0003677 DNA binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006270 DNA-dependent DNA replication initiation
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006350 transcription
- GO:0045449 regulation of transcription
- For research purposes only
Applications of MCM5 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/25 Pack Size: 0.1 mg
1/50 Pack Size: 20 µg
|1/100 Pack Size: 0.1 mg
1/ 1000 Pack Size: 20 µg
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Tonsil / Cervical Carcinoma
Secondary Antibodies Available
Application Based External Images
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for MCM5 antibody
Dart, D.A. et al. (2004) Recruitment of the cell cycle checkpoint kinase ATR to chromatin during S-phase.
J Biol Chem. 279: 16433-40.
Adams, K.E. et al. (2006) Recruitment of ATR to sites of ionising radiation-induced DNA damage requires ATM and components of the MRN protein complex.
Oncogene. 25: 3894–904.
Gakiopoulou, H. et al. (2007) Minichromosome maintenance proteins 2 and 5 in non-benign epithelial ovarian tumours: relationship with cell cycle regulators and prognostic implications.
British Journal of Cancer 97: 1124–34.
Guida, T. et al. (2005) Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma.
J Clin Endocrinol Metab. 90: 4703-9.
Polo, S.E. et al. (2010) Clinical significance and prognostic value of chromatin assembly factor-1 overexpression in human solid tumours.
Histopathology. 57: 716-24.
Williams, G.H. et al. (1998) Improved cervical smear assessment using antibodies against proteins that regulate DNA replication.
Proc Natl Acad Sci U S A. 95 (25): 14932-7.
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