MCM5 antibody | CRCT5.1
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P ResetMouse anti Human Mcm5
- Product Type
- Monoclonal Antibody
- Clone
- CRCT5.1
- Isotype
- IgG2b
- Specificity
- MCM5
Antibody Quality and 
Unrivaled 
| Mouse anti Human Mcm5 antibody, clone CRCT5.1 recognizes human Mcm-5 (minichromosome maintenance protein 5), also known as DNA replication licensing factor MCM5 or P1-CDC46. Mcm5 is a nuclear protein of ~95kDa with an important role in the control of DNA replication (Snyder et al. 2005). Immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy (Murphy et al. 2004). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1860: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1860T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bacterially expressed human Mcm5.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised CBI/CFI mice were fused with cells of the mouse SP2 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
1/25 Pack Size: 0.1 mg 1/50 Pack Size: 20 µg |
1/100 Pack Size: 0.1 mg 1/ 1000 Pack Size: 20 µg |
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human tonsil or cervical carcinoma
References for MCM5 antibody
-
(2011) Human Protein Atlas: CAB000101
Human Protein Atlas: CAB000101 -
Dart, D.A. et al. (2004) Recruitment of the cell cycle checkpoint kinase ATR to chromatin during S-phase.
J Biol Chem. 279: 16433-40. -
Adams, K.E. et al. (2006) Recruitment of ATR to sites of ionising radiation-induced DNA damage requires ATM and components of the MRN protein complex.
Oncogene. 25: 3894–904. -
Gakiopoulou, H. et al. (2007) Minichromosome maintenance proteins 2 and 5 in non-benign epithelial ovarian tumours: relationship with cell cycle regulators and prognostic implications.
British Journal of Cancer 97: 1124–34. -
Guida, T. et al. (2005) Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma.
J Clin Endocrinol Metab. 90: 4703-9. -
Polo, S.E. et al. (2010) Clinical significance and prognostic value of chromatin assembly factor-1 overexpression in human solid tumours.
Histopathology. 57: 716-24.
Further Reading
-
Williams, G.H. et al. (1998) Improved cervical smear assessment using antibodies against proteins that regulate DNA replication.
Proc Natl Acad Sci U S A. 95 (25): 14932-7.
- RRID
- AB_2235163
- UniProt
- P33992
- Entrez Gene
- MCM5
- GO Terms
- GO:0000075 cell cycle checkpoint
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000084 S phase of mitotic cell cycle
- GO:0005515 protein binding
- GO:0000216 M/G1 transition of mitotic cell cycle
- GO:0003677 DNA binding
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0006270 DNA-dependent DNA replication initiation
- View More GO Terms
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006350 transcription
- GO:0045449 regulation of transcription
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