Mast Cell Tryptase antibody | AA1

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Mouse anti Human Mast Cell Tryptase

Product Type
Monoclonal Antibody
Clone
AA1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1438T C E IF P* 20 µg
MCA1438 C E IF P* WB 0.2 mg
Mouse anti Human Mast Cell Tryptase, clone AA1 recognizes human mast cell tryptase, both alpha and beta isoforms.Mouse anti Mast cel tryptase, clone AA1 is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Walls et al. 1990).

Tryptases are the products of a number of genes and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Monkey
Cat
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human mast cell tryptase purified from human lung tissue.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P20231 Related reagents
Q15661 Related reagents
Entrez Gene
TPSB2 Related reagents
TPSAB1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
Regulatory
For research purposes only

Applications of Mast Cell Tryptase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/10,000
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

ELISA

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

Source Reference

  1. Walls, A.F. et al. (1990) Production and characterization of monoclonal antibodies specific for human mast cell tryptase.
    Clin Exp Allergy. 20 (5): 581-9.

References for Mast Cell Tryptase antibody

  1. Walls, A.F. et al. (1990) Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase.
    J Pathol. 162 (2): 119-26.
  2. Ozaki, K. et al. (2002) Mast cell tumors of the gastrointestinal tract in 39 dogs.
    Vet Pathol. 39 (5): 557-64.
  3. Jacob, C. et al. (2005) Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins.
    J Biol Chem. 280: 31936-48.
  4. Louiset, E. et al. (2008) Ectopic expression of serotonin7 receptors in an adrenocortical carcinoma co-secreting renin and cortisol.
    Endocr Relat Cancer.15: 1025-34.
  5. Xiang, M. et al. (2011) Usefulness of serum tryptase level as an independent biomarker for coronary plaque instability in a Chinese population.
    Atherosclerosis. 215 (2): 494-9.
  6. Thienemann, F. et al. (2004) Regulation of mast cell characteristics by cytokines: divergent effects of interleukin-4 on immature mast cell lines versus mature human skin mast cells.
    Arch Dermatol Res. 296: 134-8.
  7. Mauro, L.V. et al. (2008) Association between mast cells of different phenotypes and angiogenesis in colorectal cancer.
    Mol Med Report. 1: 895-902.
  8. Liu, J. et al. (2009) Genetic deficiency and pharmacological stabilization of mast cells reduce diet-induced obesity and diabetes in mice.
    Nat Med. 15: 940-5.
  9. Asano-Kato, N. et al. (2005) Tryptase increases proliferative activity of human conjunctival fibroblasts through protease-activated receptor-2.
    Invest Ophthalmol Vis Sci. 46: 4622-6.
  10. Facoetti, A. et al. (2006) Histochemical study of cardiac mast cells degranulation and collagen deposition: interaction with the cathecolaminergic system in the rat.
    Eur J Histochem. 50: 133-40.
  11. Dichlberger, A. et al. (2011) Lipid body formation during maturation of human mast cells.
    J Lipid Res. 52: 2198-208.
  12. Kawarai, S. et al. (2010) Cultivation and characterization of canine skin-derived mast cells.
    J Vet Med Sci. 72 (2): 131-40.
  13. Kazama, I. et al. (2015) Mast cell involvement in the progression of peritoneal fibrosis in rats with chronic renal failure.
    Nephrology (Carlton). 20 (9): 609-16.
  14. Sevestre, H.P. et al. (2015) Mast Cell and Follicular Lymphoma, Association or Relationship.
    Abstract 338 -Hematol Oncol. 33 Suppl 1: 263.
  15. Luo J et al. (2016) An indispensable role of CPT-1a to survive cancer cells during energy stress through rewiring cancer metabolism.
    Tumour Biol. Oct 13. [Epub ahead of print]
  16. Perbellini, O. et al. (2011) Primary role of multiparametric flow cytometry in the diagnostic work-up of indolent clonal mast cell disorders.
    Cytometry B Clin Cytom. 80 (6): 362-8.
  17. Baba, A. et al. (2016) Less Contribution of Mast Cells to the Progression of Renal Fibrosis in Rat Kidneys with Chronic Renal Failure.
    Nephrology (Carlton). Feb 2. [Epub ahead of print]
  18. Kato, Y. et al. (2016) Cutaneous mastocytosis with a mutation in the juxtamembrane domain of c-kit in a young laboratory beagle dog.
    J Toxicol Pathol. 29 (1): 49-52.