Mast Cell Tryptase antibody | AA1
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|Mouse anti Human mast cell tryptase, clone AA1 recognizes human mast cell tryptase, both alpha and beta isoforms. Mouse anti Mast cell tryptase, clone AA1 is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Walls et al. 1990).
Tryptases are the products of a number of genes and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Dog Monkey Cat Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Human mast cell tryptase purified from human lung tissue.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1||1/10,000|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
Walls, A.F. et al. (1990) Production and characterization of monoclonal antibodies specific for human mast cell tryptase.
Clin Exp Allergy. 20 (5): 581-9.
References for Mast Cell Tryptase antibody
Sevestre, H.P. et al. (2015) Mast Cell and Follicular Lymphoma, Association or Relationship.
Abstract 338 -Hematol Oncol. 33 Suppl 1: 263.
Walls, A.F. et al. (1990) Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase.
J Pathol. 162 (2): 119-26.
Ozaki, K. et al. (2002) Mast cell tumors of the gastrointestinal tract in 39 dogs.
Vet Pathol. 39 (5): 557-64.
Jacob, C. et al. (2005) Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins.
J Biol Chem. 280: 31936-48.
Louiset, E. et al. (2008) Ectopic expression of serotonin7 receptors in an adrenocortical carcinoma co-secreting renin and cortisol.
Endocr Relat Cancer.15: 1025-34.
Xiang, M. et al. (2011) Usefulness of serum tryptase level as an independent biomarker for coronary plaque instability in a Chinese population.
Atherosclerosis. 215 (2): 494-9.
Thienemann, F. et al. (2004) Regulation of mast cell characteristics by cytokines: divergent effects of interleukin-4 on immature mast cell lines versus mature human skin mast cells.
Arch Dermatol Res. 296: 134-8.
Mauro, L.V. et al. (2008) Association between mast cells of different phenotypes and angiogenesis in colorectal cancer.
Mol Med Report. 1: 895-902.
View The Latest Product References
Liu, J. et al. (2009) Genetic deficiency and pharmacological stabilization of mast cells reduce diet-induced obesity and diabetes in mice.
Nat Med. 15: 940-5.
Asano-Kato, N. et al. (2005) Tryptase increases proliferative activity of human conjunctival fibroblasts through protease-activated receptor-2.
Invest Ophthalmol Vis Sci. 46: 4622-6.
Facoetti, A. et al. (2006) Histochemical study of cardiac mast cells degranulation and collagen deposition: interaction with the cathecolaminergic system in the rat.
Eur J Histochem. 50: 133-40.
Dichlberger, A. et al. (2011) Lipid body formation during maturation of human mast cells.
J Lipid Res. 52: 2198-208.
Kawarai, S. et al. (2010) Cultivation and characterization of canine skin-derived mast cells.
J Vet Med Sci. 72 (2): 131-40.
Kazama, I. et al. (2015) Mast cell involvement in the progression of peritoneal fibrosis in rats with chronic renal failure.
Nephrology (Carlton). 20 (9): 609-16.
Kato, Y. et al. (2016) Cutaneous mastocytosis with a mutation in the juxtamembrane domain of c-kit in a young laboratory beagle dog.
J Toxicol Pathol. 29 (1): 49-52.
Luo, J. et al. (2016) An indispensable role of CPT-1a to survive cancer cells during energy stress through rewiring cancer metabolism.
Tumour Biol. Oct 13 [Epub ahead of print].
Perbellini, O. et al. (2011) Primary role of multiparametric flow cytometry in the diagnostic work-up of indolent clonal mast cell disorders.
Cytometry B Clin Cytom. 80 (6): 362-8.
Baba, A. et al. (2017) Less contribution of mast cells to the progression of renal fibrosis in Rat kidneys with chronic renal failure.
Nephrology (Carlton). 22 (2): 159-67.
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