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MAdCAM-1 antibody | 314G8

Mouse anti Human MAdCAM-1

Product Type
Monoclonal Antibody
Clone
314G8
Isotype
IgG1
Specificity
MAdCAM-1

Product Code Applications Pack Size List Price Your Price Qty
MCA2320T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F 25 µg loader
List Price Your Price
loader
MCA2320
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.2 mg loader
List Price Your Price
loader

Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA2320T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA2320: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant soluble MAdCAM-1-fc fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/100
Immunohistology - Frozen 1/25 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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>
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Description Mouse IgG1 Negative Control

References for MAdCAM-1 antibody

  1. Leung, E. et al. (1996) Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts.
    Immunol Cell Biol. 74 (6): 490-6.
  2. Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
    Immunol Cell Biol. 82 (4): 400-9.
  3. Olloquequi, J. et al. (2011) Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients.
    Eur Respir J. 37 (2): 289-98.
  4. Guerra-Pérez, N. et al. (2015) Retinoic Acid Imprints a Mucosal-like Phenotype on Dendritic Cells with an Increased Ability To Fuel HIV-1 Infection.
    J Immunol. pii: 1402623.
  5. Goode, D. et al. (2014) Sex hormones selectively impact the endocervical mucosal microenvironment: implications for HIV transmission.
    PLoS One. 9 (5): e97767.
  6. Guzzo, C. et al. (2017) Virion incorporation of integrin αα4β7 facilitates HIV-1 infection and intestinal homing.
    Sci Immunol. 2 (11) [Epub ahead of print].

Synonyms
Mucosal Addressin Cell Adhesion MOLECULE-1
RRID
AB_567120
UniProt
Q13477
Entrez Gene
MADCAM1
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005624 membrane fraction
GO:0006955 immune response
GO:0050776 regulation of immune response

MCA2320T

158078 1805

MCA2320

0414 152845 168032

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