LOX-1 antibody | DE15-4H4

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Mouse anti Human LOX-1

Product Type
Monoclonal Antibody
Clone
DE15-4H4
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6250GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human LOX-1 antibody, clone DE15-4H4, recognizes lectin-like oxidized LDL receptor 1 (LOX-1) also known as oxidized low density lipoprotein receptor 1 and C-type lectin domain family 8 member A. LOX-1 is a 5 kDa transmembrane glycoprotein and is a member of the class E of the scavenger receptors. It is expressed in a variety of cells including macrophages, vascular smooth muscle cells, cardiomyocytes, platelets and fibroblasts (Kattoor et al. 2019). Proteolytic cleavage of LOX-1 results in the soluble form sLOX-1. The ligand Ox-LDL exerts its biological effects through the LOX-1 receptor, with its binding resulting in induction of the MAPK or NF-κB signaling pathways. Downstream of this, these pathways can trigger the induction of the productions of adhesion molecules and pro-inflammatory cytokines (https://pubmed.ncbi.nlm.nih.gov/30819724/).

LOX-1 is expressed at low levels under normal physiological conditions, but is upregulated in various pathological conditions including atherosclerosis, diabetes mellitus, hypertension and dyslipidemia (Kattoor et al. 2019).

The purified Mouse anti Human LOX-1 antibody, clone DE15-4H4 (MCA6250GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human LOX-1 antibody, clone DE17-4B9 (MCA6251B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
HEK293 derived recombinant human full length LOX-1 (Ser61-Gln273)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P78380
Entrez Gene
OLR1
GO Terms
GO:0007596 blood coagulation
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0005624 membrane fraction
GO:0006508 proteolysis
GO:0006954 inflammatory response
GO:0008015 blood circulation
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of LOX-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human LOX-1:Biotin MCA6251B E 0.1 mg loader

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