KRAS antibody | 3B10-2F2
Mouse anti Human KRAS antibody, clone 3B10-2F2 recognizes human KRAS, also known as GTPase KRas or c-Ki-ras. KRAS is a 189 amino acid ~21 kDa membrane bound cytosolic protein involved in the regulation of cell proliferation. Defects in the KRAS gene have beel implicated in leukemias, Noonan syndrome and gastric cancer (UniProt: P01116).
Mouse anti Human KRAS antibody, clone 3B10-2F2 has been used successfully for the domonstration of KRAS expression in malignant peripheral nerve sheath tumor cell lysates by western blotting (Miller et al. 2013).
Mouse anti Human KRAS antibody, clone 3B10-2F2 has been used successfully for the domonstration of KRAS expression in malignant peripheral nerve sheath tumor cell lysates by western blotting (Miller et al. 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1 - 189 of human KRAS.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01116 Related reagents
- Entrez Gene
- KRAS Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0000186 activation of MAPKK activity
- GO:0003924 GTPase activity
- GO:0007596 blood coagulation
- GO:0005525 GTP binding
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008286 insulin receptor signaling pathway
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of KRAS antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml | |
| Western Blotting | 0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for KRAS antibody
-
Kohli, L .et al. (2013) 4-Hydroxytamoxifen induces autophagic death through K-Ras degradation.
Cancer Res. 73 (14): 4395-405. -
Brossier, N.M. et al. (2015) Classic Ras Proteins Promote Proliferation and Survival via Distinct Phosphoproteome Alterations in Neurofibromin-Null Malignant Peripheral Nerve Sheath Tumor Cells.
J Neuropathol Exp Neurol. 74 (6): 568-86.
