KRAS antibody | 3B10-2F2

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Anti Human KRAS Antibody, clone 3B10-2F2 thumbnail image 1

Anti Human KRAS Antibody, clone 3B10-2F2 thumbnail image 2

Anti Human KRAS Antibody, clone 3B10-2F2 gallery image 1 — Immunofluorescence showing KRAS in HeLa cells using preservative free Mouse anti Human KRAS antibody, clone 3B10-2F2 (**MCA3223Z**).

Anti Human KRAS Antibody, clone 3B10-2F2 gallery image 2 — Western blot analysis of KRAS expression in HeLa cell lysate using preservative free Mouse anti Human KRAS antibody, clone 3B10-2F2 (**MCA3223Z**).

Mouse anti Human KRAS:Preservative Free

Product Type: Monoclonal Antibody
Clone: 3B10-2F2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA3223Z	IF WB			0.1 mg

Mouse anti Human KRAS antibody, clone 3B10-2F2 recognizes human KRAS, also known as GTPase KRas or c-Ki-ras. KRAS is a 189 amino acid ~21 kDa membrane bound cytosolic protein involved in the regulation of cell proliferation. Defects in the KRAS gene have beel implicated in leukemias, Noonan syndrome and gastric cancer (UniProt: P01116).

Mouse anti Human KRAS antibody, clone 3B10-2F2 has been used successfully for the domonstration of KRAS expression in malignant peripheral nerve sheath tumor cell lysates by western blotting (Miller et al. 2013).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant protein corresponding to aa 1 - 189 of human KRAS.
Approx. Protein Concentrations: Ig concentration 0.5 mg/ml
Fusion Partners: Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01116 Related reagents
Entrez Gene: KRAS Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0000186 activation of MAPKK activity; GO:0003924 GTPase activity; GO:0007596 blood coagulation; GO:0005525 GTP binding; GO:0007173 epidermal growth factor receptor signaling pathway; GO:0007265 Ras protein signal transduction; GO:0007411 axon guidance; GO:0008286 insulin receptor signaling pathway; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050900 leukocyte migration
Regulatory: For research purposes only

Applications of KRAS antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence		0.1	10 ug/ml
Western Blotting		0.1	10 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for KRAS antibody

Kohli, L .et al. (2013) 4-Hydroxytamoxifen induces autophagic death through K-Ras degradation.
Cancer Res. 73 (14): 4395-405.
Brossier, N.M. et al. (2015) Classic Ras Proteins Promote Proliferation and Survival via Distinct Phosphoproteome Alterations in Neurofibromin-Null Malignant Peripheral Nerve Sheath Tumor Cells.
J Neuropathol Exp Neurol. 74 (6): 568-86.

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