KIR antibody | NKVFS1

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Anti Human KIR Antibody, clone NKVFS1 thumbnail image 1

Anti Human KIR Antibody, clone NKVFS1 thumbnail image 2

Anti Human KIR Antibody, clone NKVFS1 gallery image 1 — Figure A. FITC conjugated mouse anti human CD3 (MCA2184F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti human CD3 (MCA2184F) and RPE conjugated mouse anti human CD158a (MCA2243PE). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

Anti Human KIR Antibody, clone NKVFS1 gallery image 2 — Figure A. Alexa647® conjugated mouse anti human CD56 (MCA2693A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. Alexa647® conjugated mouse anti human CD56 (MCA2693A647) and RPE conjugated mouse anti human CD158a (MCA2243PE). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock (BUF070A).

Mouse anti Human KIR:FITC

Mouse anti Human KIR:Low Endotoxin

Mouse anti Human KIR

Mouse anti Human KIR:RPE

Product Type: Monoclonal Antibody
Clone: NKVFS1
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2243F	F	0.1 mg
MCA2243EL	E F FN IP WB	0.5 mg
MCA2243T	E F IP WB	25 µg
MCA2243GA	E F IP WB	0.1 mg
MCA2243	E F IP WB	0.2 mg
MCA2243PET	F	25 Tests
MCA2243PE	F	100 Tests

Mouse anti Human KIR antibody, clone NKVFS1 recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family, CD158a, CD158b and P50.3. KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.

Mouse anti Human KIR antibody, clone NKVFS1 recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4).

Mouse anti Human KIR antibody, clone NKVFS1 is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms..

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests

Reconstitute in 0.25 ml disilled water

Pack Size: 100 Tests

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^o only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P43626 Related reagents; P43627 Related reagents; P43628 Related reagents; Q14954 Related reagents; P43631 Related reagents; P43632 Related reagents
Entrez Gene: KIR2DL1 Related reagents; KIR2DL2 Related reagents; KIR2DL3 Related reagents; KIR2DS2 Related reagents; KIR2DS4 Related reagents; KIR2DS1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0002769 natural killer cell inhibitory signaling pathway; GO:0004872 receptor activity; GO:0005887 integral to plasma membrane; GO:0006955 immune response; GO:0005886 plasma membrane; GO:0016021 integral to membrane; GO:0050776 regulation of immune response; GO:0003823 antigen binding; GO:0004888 transmembrane receptor activity
Regulatory: For research purposes only

Applications of KIR antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/5
ELISA
Flow Cytometry	1/50	1/100
Functional Assays
Immunoprecipitation
Western Blotting
ELISA
Flow Cytometry	1/50	1/100
Immunoprecipitation
Western Blotting
Flow Cytometry		Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control:Low Endotoxin	MCA928EL	0.5 mg	C F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for KIR antibody

Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
Blood. 99 (5): 1706-14.
Spaggiari, G.M. et al. (2002) Soluble HLA class I induces NK cell apoptosis upon the engagement of killer-activating HLA class I receptors through FasL-Fas interaction.
Blood. 100 (12): 4098-107.
Older Aguilar, A.M. et al. (2010) Coevolution of killer cell Ig-like receptors with HLA-C to become the major variable regulators of human NK cells.
J Immunol. 185 (7): 4238-51.
Patterson, S. et al. (2008) Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors.
J Immunol. 181 (5): 3268-76.
Hilton, H.G. et al. (2015) The production of KIR-Fc fusion proteins and their use in a multiplex HLA class I binding assay.
J Immunol Methods. 425: 79-87.
Moesta, A.K. et al. (2009) Chimpanzees use more varied receptors and ligands than humans for inhibitory killer cell Ig-like receptor recognition of the MHC-C1 and MHC-C2 epitopes.
J Immunol. 182 (6): 3628-37.
Van Der Ploeg, K. et al. (2017) Modulation of Human Leukocyte Antigen-C by Human Cytomegalovirus Stimulates KIR2DS1 Recognition by Natural Killer Cells.
Front Immunol. 8: 298.
Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
Immunol Invest. 38 (5): 434-46.

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