KIR antibody | NKVFS1


Mouse anti Human KIR:FITC
Mouse anti Human KIR:Low Endotoxin
Mouse anti Human KIR
Mouse anti Human KIR:RPE
- Product Type
- Monoclonal Antibody
- Clone
- NKVFS1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2243F | F | 0.1 mg |
![]() |
|
MCA2243EL | E F FN IP WB | 0.5 mg |
![]() |
|
MCA2243 | E F IP WB | 0.2 mg |
![]() |
|
MCA2243GA | E F IP WB | 0.1 mg |
![]() |
|
MCA2243PE | F | 100 Tests |
![]() |
Mouse anti Human KIR antibody, clone NKVFS1 recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4).
Mouse anti Human KIR antibody, clone NKVFS1 is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms..
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20o only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P43626
- P43627
- P43628
- Q14954
- P43631
- P43632
- Entrez Gene
- KIR2DL1
- KIR2DL2
- KIR2DL3
- KIR2DS2
- KIR2DS4
- KIR2DS1
- GO Terms
- GO:0005515 protein binding
- GO:0002769 natural killer cell inhibitory signaling pathway
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
- GO:0003823 antigen binding
- GO:0004888 transmembrane receptor activity
- Regulatory
- For research purposes only
Applications of KIR antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 | |
ELISA | |||
Flow Cytometry | 1/50 | 1/100 | |
Functional Assays | |||
Immunoprecipitation | |||
Western Blotting | |||
ELISA | |||
Flow Cytometry | 1/50 | 1/100 | |
Immunoprecipitation | |||
Western Blotting | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
![]() |
|
Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
![]() |
|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
![]() |
|
Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for KIR antibody
-
Spaggiari, G.M. et al. (2002) Soluble HLA class I molecules induce natural killer cell apoptosis through the engagement of CD8: evidence for a negative regulation exerted by members of the inhibitory receptor superfamily.
Blood. 99 (5): 1706-14. -
Spaggiari, G.M. et al. (2002) Soluble HLA class I induces NK cell apoptosis upon the engagement of killer-activating HLA class I receptors through FasL-Fas interaction.
Blood. 100 (12): 4098-107. -
Older Aguilar, A.M. et al. (2010) Coevolution of killer cell Ig-like receptors with HLA-C to become the major variable regulators of human NK cells.
J Immunol. 185 (7): 4238-51. -
Patterson, S. et al. (2008) Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors.
J Immunol. 181 (5): 3268-76. -
Hilton, H.G. et al. (2015) The production of KIR-Fc fusion proteins and their use in a multiplex HLA class I binding assay.
J Immunol Methods. 425: 79-87. -
Moesta, A.K. et al. (2009) Chimpanzees use more varied receptors and ligands than humans for inhibitory killer cell Ig-like receptor recognition of the MHC-C1 and MHC-C2 epitopes.
J Immunol. 182 (6): 3628-37. -
Van Der Ploeg, K. et al. (2017) Modulation of Human Leukocyte Antigen-C by Human Cytomegalovirus Stimulates KIR2DS1 Recognition by Natural Killer Cells.
Front Immunol. 8: 298. -
Wang, Y. et al. (2009) Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.
Immunol Invest. 38 (5): 434-46. -
Wijaya, R.S. et al. (2020) Expansion of dysfunctional CD56-CD16+ NK cells in chronic hepatitis B patients.
Liver Int. Dec 23 [Epub ahead of print].
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra