JARID1B antibody | 1G10

Mouse anti Human JARID1B:Preservative Free

Product Type
Monoclonal Antibody
Clone
1G10
Isotype
IgG2a
Specificity
JARID1B

Product Code Applications Pack Size List Price Your Price Qty
MCA4340Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human JARID1B antibody, clone 1G10 recognizes human Lysine-specific demethylase 5B, also known as JARID1B, Cancer/testis antigen 31, Histone demethylase JARID1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1 or Jumonji/ARID domain-containing protein 1B. JARID1B is a 1544 amino acid ~180 kDa ubiquitously expressed histone demethylase.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 231-320 of human JARID1B
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting 0.1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for JARID1B antibody

  1. Xiang, Y. et al. (2007) JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.
    Proc Natl Acad Sci U S A. 104: 19226-31.
  2. Kuźbicki, L. et al. (2013) JARID1B expression in human melanoma and benign melanocytic skin lesions.
    Melanoma Res. 23 (1): 8-12.
  3. Kużbicki, Ł. et al. (2017) Prognostic significance of RBP2-H1 variant of JARID1B in melanoma.
    BMC Cancer. 17 (1): 854.

Synonyms
Lysine Specific Demethylase 5B
RRID
AB_1221667
UniProt
Q9UGL1
Entrez Gene
KDM5B
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003714 transcription corepressor activity
GO:0005730 nucleolus
GO:0055114 oxidation-reduction process
GO:0008270 zinc ion binding
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0034647 histone demethylase activity (H3-trimethyl-K4 specific)
GO:0034648 histone demethylase activity (H3-dimethyl-K4 specific)
GO:0045892 negative regulation of transcription, DNA-dependent
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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