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IRF3 antibody | 3F10

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Anti Human IRF3 Antibody, clone 3F10 thumbnail image 1 Anti Human IRF3 Antibody, clone 3F10 thumbnail image 2
Anti Human IRF3 Antibody, clone 3F10 gallery image 1
Western blot analysis of IRF3 expression on a Jurkat cell lysate using Mouse anti Human IRF3 (MCA2700)
Anti Human IRF3 Antibody, clone 3F10 gallery image 2
Imminoprecipitaion of IRF3 fron HeLa cells using Mouse anti Human IRF3 (MCA2700)
Lane A: Normal IgG
Lane B: Mouse anti Human IRF3, clone 3F10
(non-specific bands represent IgG H+L in the preparation)

Immunofluorescent staining of HeLa cells with Mouse anti Human IRF3 (MCA2700), IRF3 (Red), nuclei counterstained with Hoescht 3342 (blue)

Mouse anti Human IRF3

Product Type
Monoclonal Antibody
Clone
3F10
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2700
Datasheet
E IP WB
MSDS
0.1 mg loader

Mouse anti Human IRF3 antibody, clone 3F10 recognizes human interferon regulatory factor 3 (IRF3), a ~55 kDa constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity (Nociari et al. 2007).

IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta and subsequent downstream interferon-induced genes (Weaver et al. 1998).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
10% Glycerol
Immunogen
Recombinant human IRF3 (amino acids 108-166) purified from E. coli.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q14653 Related reagents
Entrez Gene
IRF3 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003702 RNA polymerase II transcription factor activity
GO:0003712 transcription cofactor activity
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0006355 regulation of transcription, DNA-dependent
GO:0006366 transcription from RNA polymerase II promoter
GO:0008063 Toll signaling pathway
GO:0010008 endosome membrane
GO:0032480 negative regulation of type I interferon production
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
Regulatory
For research purposes only

Applications of IRF3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoprecipitation
Western Blotting 1/1000 1/3000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2700 detects a band of approximately 55kDa in HeLa cell lysates.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Further Reading

  1. Takahasi, K. et al. (2003) X-ray crystal structure of IRF-3 and its functional implications.
    Nat Struct Biol. 10 (11): 922-7.
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