IRF3 antibody | 3F10

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Anti Human IRF3 Antibody, clone 3F10 thumbnail image 1

Anti Human IRF3 Antibody, clone 3F10 thumbnail image 2

Anti Human IRF3 Antibody, clone 3F10 gallery image 1 — Western blot analysis of IRF3 expression on a Jurkat cell lysate using Mouse anti Human IRF3 (MCA2700)

Anti Human IRF3 Antibody, clone 3F10 gallery image 2 — Imminoprecipitaion of IRF3 fron HeLa cells using Mouse anti Human IRF3 (MCA2700)
Lane A: Normal IgG
Lane B: Mouse anti Human IRF3, clone 3F10
(non-specific bands represent IgG H+L in the preparation)

Immunofluorescent staining of HeLa cells with Mouse anti Human IRF3 (MCA2700), IRF3 (Red), nuclei counterstained with Hoescht 3342 (blue)

Mouse anti Human IRF3

Product Type: Monoclonal Antibody
Clone: 3F10
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2700	E IP WB			0.1 mg

Mouse anti Human IRF3 antibody, clone 3F10 recognizes human interferon regulatory factor 3 (IRF3), a ~55 kDa constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity (Nociari et al. 2007).

IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta and subsequent downstream interferon-induced genes (Weaver et al. 1998).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Sodium Azide (NaN₃)
10% Glycerol
Immunogen: Recombinant human IRF3 (amino acids 108-166) purified from E. coli.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: Q14653 Related reagents
Entrez Gene: IRF3 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0003712 transcription cofactor activity; GO:0009615 response to virus; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0006355 regulation of transcription, DNA-dependent; GO:0006366 transcription from RNA polymerase II promoter; GO:0008063 Toll signaling pathway; GO:0010008 endosome membrane; GO:0032480 negative regulation of type I interferon production; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060337 type I interferon-mediated signaling pathway
Regulatory: For research purposes only

Applications of IRF3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Immunoprecipitation
Western Blotting	1/1000	1/3000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: MCA2700 detects a band of approximately 55kDa in HeLa cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F