IP-10 antibody | A02-1E10

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Mouse anti Human IP-10

Product Type
Monoclonal Antibody
Clone
A02-1E10
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5985GA E 0.1 mg
Mouse anti Human IP-10, clone A02-1E10 recognizes the chemokine IP-10. IP-10, also known as CXCL10, is a small cytokine belonging to the CXC chemokine family. IP-10 cDNA encodes a 98 amino acid protein which is cleaved and secreted from a wide variety of cell types in response to interferon gamma and other inflammatory cytokines (Ohmori & Hamilton 1995).

IP-10 exerts its biological effects mainly via binding to CXCR3 (van den Borne et al. 2014). Also, CXCR3-independent pathways have been studied for IP-10/CXCL10 involving binding to heparan sulfate glycosaminoglycans (GAGs). IP-10 not only induces chemoattraction of inflammatory cells, but also migration and proliferation of endothelial cells and VSMCs (Wang et al. 1996).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from serum free tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli derived recombinant protein corresponding to aa 22-98 of human IP-10
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P02778 Related reagents
Entrez Gene
CXCL10 Related reagents
GO Terms
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0007166 cell surface receptor linked signaling pathway
GO:0007267 cell-cell signaling
GO:0007517 muscle organ development
GO:0008009 chemokine activity
GO:0008015 blood circulation
GO:0008284 positive regulation of cell proliferation
GO:0008603 cAMP-dependent protein kinase regulator activity
Regulatory
For research purposes only

Applications of IP-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Further Reading

  1. van den Borne, P. et al. (2014) The multifaceted functions of CXCL10 in cardiovascular disease
    Biomed Res Int. 2014: 893106.
  2. Ohmori, Y. & Hamilton, T.A. (1994) Cell type and stimulus specific regulation of chemokine gene expression
    Biochem Biophys Res Commun. 198: 590-6.
  3. Wang, X. et al. (1996) Interferon-inducible protein-10 involves vascular smooth muscle cell migration, proliferation, and inflammatory response
    J Biol Chem. 271: 24286-93.