Inhibin Alpha antibody | R1

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Mouse anti Human Inhibin Alpha

Product Type
Monoclonal Antibody
Clone
R1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA951ST C P* 0.2 ml
MCA951S C P* WB 2 ml
Mouse anti Human inhibin alpha antibody, clone R1 recognizes the alpha subunit of the 32 kDa human inhibin. A glycoprotein which acts to suppress follicle-stimulating hormone (FSH) secretion (Groome et al, 1990).

Inhibin alpha is expressed in a range of tissues including the endometrium (Mylonas et al, 2004), brain (Uccella et al, 2000), adrenal gland (McCluggage et al, 1998), testis (Bergh and Cajander, 1990) and ovary (Cuevas et al, 1987, Yamoto et al, 1992). The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions, and sex cord stromal tumours (McCluggage, 2001).

In western blotting of bovine follicular fluid, bands at 20, 44 and 57 kDa were reported under reducing conditions using clone R1 (Groome et al, 1991).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
Pack Size: 0.2 ml
0.09% Sodium Azide (NaN3)
Pack Size: 2 ml
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide comprised of amino acids 1-32 of human inhibin alpha.

Storage Information

Storage
Pack Size: 0.2 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 2 ml
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05111 Related reagents
Entrez Gene
INHA Related reagents
GO Terms
GO:0001501 skeletal system development
GO:0001541 ovarian follicle development
GO:0004871 signal transducer activity
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007166 cell surface receptor linked signaling pathway
GO:0007267 cell-cell signaling
GO:0007399 nervous system development
GO:0008083 growth factor activity
GO:0009605 response to external stimulus
GO:0046881 positive regulation of follicle-stimulating hormone secretion
GO:0046882 negative regulation of follicle-stimulating hormone secretion
GO:0030218 erythrocyte differentiation
GO:0034673 inhibin-betaglycan-ActRII complex
GO:0042127 regulation of cell proliferation
GO:0042326 negative regulation of phosphorylation
GO:0042541 hemoglobin biosynthetic process
GO:0043512 inhibin A complex
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0045578 negative regulation of B cell differentiation
GO:0045650 negative regulation of macrophage differentiation
Regulatory
For research purposes only

Applications of Inhibin Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen Neat 1/100
Immunohistology - Paraffin 1 Neat 1/100
Western Blotting 1/100 1/200
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Normal human ovary/testis

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

Source Reference

  1. Groome N et al. (1990) Monoclonal and polyclonal antibodies reactive with the 1-32 amino terminal sequence of the alpha subunit of human 32K inhibin.
    Hybridoma. 9 (1): 31-42.

References for Inhibin Alpha antibody

  1. Groome, N. & Lawrence, M. (1991) Preparation of monoclonal antibodies to the beta A subunit of ovarian inhibin using a synthetic peptide immunogen.
    Hybridoma. 10 (2): 309-16.
  2. Arora, D.S. et al. (1997) Immunohistochemical expression of inhibin/activin subunits in epithelial and granulosa cell tumours of the ovary.
    J Pathol. 181 (4): 413-8.
  3. Mylonas, I. et al. (2009) Inhibin-alpha subunit is an independent prognostic parameter in human endometrial carcinomas: analysis of inhibin/activin-alpha, -betaA and -betaB subunits in 302 cases.
    Eur J Cancer. 45 (7): 1304-14.
  4. Schraith, D.F. et al. (2003) Alpha-inhibin immunoreactivity in soft-tissue neoplasia.
    Mod Pathol. 16 (12): 1205-9.
  5. Akiyama, T. et al. (2009) Pancreatic serous microcystic adenoma with extensive oncocytic change.
    Pathol Int. 59 (2): 102-6.
  6. Choi, Y.L. et al. (2000) Immunoexpression of inhibin alpha subunit, inhibin/activin betaA subunit and CD99 in ovarian tumors.
    Arch Pathol Lab Med. 124 (4): 563-9.
  7. Hayashi, K. et al. (2003) The activin-follistatin system in the neonatal ovine uterus.
    Biol Reprod. 69 (3): 843-50.
  8. Karlberg, S. et al. (2011) Testicular failure and male infertility in the monogenic Mulibrey nanism disorder.
    J Clin Endocrinol Metab. 96 (11): 3399-407.
  9. Vanmontfort, D. et al. (1998) cDNA sequence analysis, gene expression and protein localisation of the inhibin alpha-subunit of Australian brushtail possum (Trichosurus vulpecula).
    J Mol Endocrinol. 21 (2): 141-52.
  10. Katoh, T. et al. (2012) Estrogen-producing endometrioid adenocarcinoma resembling sex cord-stromal tumor of the ovary: a review of four postmenopausal cases.
    Diagn Pathol. 7: 164.
  11. Biancani, B. et al. (2010) Ovarian interstitial cell tumor in a South American sea lion (Otaria flavescens).
    J Wildl Dis. 46: 1012-6.
  12. Marino, F.E. et al. (2014) The inhibin/activin signalling pathway in human gonadal and adrenal cancers.
    Mol Hum Reprod. 20 (12): 1223-37.
  13. Wang Y et al. (2016) Follicle Depletion Provides A Permissive Environment for Ovarian Carcinogenesis.
    Mol Cell Biol. Jun 27. pii: MCB.00202-16. [Epub ahead of print]
  14. Canadas A. et al. (2016) Multiple Cutaneous Metastasis of a Malignant Leydig Cell Tumour in a Dog
    Journal of Comparative Pathology. Jul 5 [Epub ahead of print]
  15. Bae, G.E. et al. (2016) Placenta percreta with small bowel invasion: unusual presentation of abnormal placental adherence
    Int J Clin Exp Pathol 9(7):7237-43
  16. Liang, L. et al. (2016) Renal cell carcinoma metastatic to the ovary or fallopian tube: a clinicopathological study of 9 cases.
    Hum Pathol. 51: 96-102.
  17. Stewart CJ et al. (2016) SF1 immunohistochemistry is useful in differentiating uterine tumours resembling sex cord-stromal tumours from potential histological mimics.
    Pathology. 48 (5): 434-40.
  18. Paulini, F. et al. (2016) Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting.
    Reprod Biomed Online. May 13. pii: S1472-6483(16)30294-2. [Epub ahead of print]
  19. Lai, J.P. et al. (2015) Isolated Large Cell Calcifying Sertoli Cell Tumor in a Young Boy, not Associated with Peutz-Jeghers Syndrome or Carney Complex.
    Ann Clin Lab Res. 3 (1): 2.
  20. Strehl, J.D. et al. (2015) Pattern of SMARCB1 (INI1) and SMARCA4 (BRG1) in poorly differentiated endometrioid adenocarcinoma of the uterus: analysis of a series with emphasis on a novel SMARCA4-deficient dedifferentiated rhabdoid variant.
    Ann Diagn Pathol. 19 (4): 198-202.
  21. Solomon LW & Velez I (2016) S-100 Negative Granular Cell Tumor of the Oral Cavity.
    Head Neck Pathol. 10 (3): 367-73.
  22. Evron, S. et al. (2007) Activin betaA in term placenta and its correlation with placental inflammation in parturients having epidural or systemic meperidine analgesia: a randomized study.
    J Clin Anesth. 19 (3): 168-74.
  23. An, S. et al. (2015) Granular cell tumor of the gastrointestinal tract: histologic and immunohistochemical analysis of 98 cases.
    Hum Pathol. 46 (6): 813-9.
  24. Bae, G.E. et al. (2016) Placenta percreta with small bowel invasion: unusual presentation of abnormal placental adherence.
    Int J Clin Exp Pathol 9 (7):7237-43.
  25. Wakayama, A. et al. (2017) Recurrent female adnexal tumor of probable Wolffian origin treated with debulking surgery, imatinib and paclitaxel/carboplatin combination chemotherapy: A case report.
    Oncology Letters. Mar 17 [Epub ahead of print]
  26. Rekhi, B. et al. (2012) Alveolar soft part sarcoma 'revisited': clinicopathological review of 47 cases from a tertiary cancer referral centre, including immunohistochemical expression of TFE3 in 22 cases and 21 other tumours.
    Pathology. 44 (1): 11-7.
  27. Polinas, M. et al. (2016) Co-occurrence of a metastatic mammary liposarcoma and an ovarian sex-cord stromal tumor in a dog.
    Res Vet Sci. 109: 157-60.