Inhibin Alpha antibody | R1
Mouse anti human inhibin alpha antibody, clone R1 (MCA951S) used for the demonstration of inhibin expression in an adenomatoid tumour by immunohistochemistry.
Image caption:
Immunohistochemical staining of tumour within adrenal gland. Inhibin antibody staining (Bio-Rad, UK, dilution 1:10).
From: H. El-Daly, P. Rao, F. Palazzo, and M. Gudi, “A Rare Entity of an Unusual Site: Adenomatoid Tumour of the Adrenal Gland: A Case Report and Review of the Literature,”
Pathology Research International, vol. 2010, Article ID 702472
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human inhibin alpha antibody, clone R1 (MCA951S) used to demonstrate inhibin expression in an endometrioid adenocarcinoma by immunohistochemistry.
Image caption:
Immunohistochemically, the carcinoma cells are clearly positive for CK7 (a), ER (b), while many stromal cells show positive reaction for inhibin-α (c) (Case 3). Expression of steroid factor-1 (SF-1), which is a transcription factor alternatively termed adrenal 4-binding protein (Ad4BP) to regulate expression of all steroidogenic enzymes [9], is predominant in the stromal cells but minimal in epithelial cells (Case 1).
From: Katoh T, Yasuda M, Hasegawa K, Kozawa E, Maniwa J, Sasano H.Estrogen-producing endometrioid adenocarcinoma resembling sex cord-stromal tumor of the ovary: a review of four postmenopausal cases.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human inhibin alpha antibody, clone R1 (MCA951S) used to identify inhibin alpha expressing cells in Sertoli tumors by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry of the large cell calcifying sertoli tumor. Left panel with low power (x40) shows indicated protein immunoreactivities in large cell calcifying sertoli cell tumor tissue at the left side (T) and in the adjacent benign sertoli cell tissue at the right side (SC). Middle panel shows the indicated protein immunoreactivities in the LCCSCT (x200) and right panel shows the indicated protein immunoreactivities in the benign sertoli cells (x200).
From: Lai JP, Lee CC, Crocker M, Najmuddin M, Lange E, Merino M, Stratakis CA.
Isolated Large Cell Calcifying Sertoli Cell Tumor in a Young Boy, not Associated with Peutz-Jeghers Syndrome or Carney Complex.
Ann Clin Lab Res. 2015;3(1):2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human inhibin alpha antibody, clone R1 (MCA951S) used to indicate inhibin alpha staininf in mouse ovaries by immuonhistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
c and d Immunohistochemical localization of INHA in PD7 control and TGFBR1-CAG9Cre ovaries. Arrows indicate abnormal follicle structures. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Four independent samples per group were used for immunohistochemical analyses. Scale bar is representatively depicted in (c) and equals 50 μm (c and d)
From: Gao Y, Fang X, Vincent DF, Threadgill DW, Bartholin L, Li Q.
Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling.
Reprod Biol Endocrinol. 2017 Dec 8;15(1):94.
doi: 10.1186/s12958-017-0312-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human inhibin alpha antibody, clone R1 (MCA951S) used to indicate inhibin alpha staininf in mouse ovaries by immuonhistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of ovarian tumor markers. a-j Expression of granulosa cell and germ cell markers in 8-week-old control and TGFBR1-CAG9Cre ovaries. Representative images from immunohistochemical analysis of FOXL2 (a and b), INHA (c and d), FOXO1 (e and f), AMH (g and h), and DDX4 (i and j) are shown. k and l Negative controls using isotype-matched rabbit and goat IgGs. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Five independent samples per group were utilized in this analysis. Scale bar is representatively depicted in (a) and equals 50 μm (a-l)
From: Gao Y, Fang X, Vincent DF, Threadgill DW, Bartholin L, Li Q.
Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling.
Reprod Biol Endocrinol. 2017 Dec 8;15(1):94.
doi: 10.1186/s12958-017-0312-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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P C ResetMouse anti Human Inhibin Alpha
- Product Type
- Monoclonal Antibody
- Clone
- R1
- Isotype
- IgG2a
- Specificity
- Inhibin Alpha
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Antibody Quality and 
Unrivaled 
| Mouse anti Human inhibin alpha antibody, clone R1 recognizes the alpha subunit of the 32 kDa human inhibin. A glycoprotein which acts to suppress follicle-stimulating hormone (FSH) secretion (Groome et al, 1990). Inhibin alpha is expressed in a range of tissues including the endometrium (Mylonas et al, 2004), brain (Uccella et al, 2000), adrenal gland (McCluggage et al, 1998), testis (Bergh and Cajander, 1990) and ovary (Cuevas et al, 1987, In western blotting of bovine follicular fluid, bands at 20, 44 and 57 kDa were reported under reducing conditions using clone R1 (Groome et al, 1991). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide comprised of amino acids 1-32 of human inhibin alpha.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
Neat | 1/100 |
| Immunohistology - Paraffin 1 | |
Neat | 1/100 |
| Western Blotting | |
1/100 | 1/200 |
- 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Normal human ovary/testis
Source Reference
-
Groome N et al. (1990) Monoclonal and polyclonal antibodies reactive with the 1-32 amino terminal sequence of the alpha subunit of human 32K inhibin.
Hybridoma. 9 (1): 31-42.
References for Inhibin Alpha antibody
-
(2011) Human Protein Atlas: CAB000047
Human Protein Atlas: CAB000047 -
Groome, N. & Lawrence, M. (1991) Preparation of monoclonal antibodies to the beta A subunit of ovarian inhibin using a synthetic peptide immunogen.
Hybridoma. 10 (2): 309-16. -
Arora, D.S. et al. (1997) Immunohistochemical expression of inhibin/activin subunits in epithelial and granulosa cell tumours of the ovary.
J Pathol. 181 (4): 413-8. -
Mylonas, I. et al. (2009) Inhibin-alpha subunit is an independent prognostic parameter in human endometrial carcinomas: analysis of inhibin/activin-alpha, -betaA and -betaB subunits in 302 cases.
Eur J Cancer. 45 (7): 1304-14. -
Schraith, D.F. et al. (2003) Alpha-inhibin immunoreactivity in soft-tissue neoplasia.
Mod Pathol. 16 (12): 1205-9. -
Akiyama, T. et al. (2009) Pancreatic serous microcystic adenoma with extensive oncocytic change.
Pathol Int. 59 (2): 102-6. -
Choi, Y.L. et al. (2000) Immunoexpression of inhibin alpha subunit, inhibin/activin betaA subunit and CD99 in ovarian tumors.
Arch Pathol Lab Med. 124 (4): 563-9. -
Hayashi, K. et al. (2003) The activin-follistatin system in the neonatal ovine uterus.
Biol Reprod. 69 (3): 843-50.
View The Latest Product References
-
Karlberg, S. et al. (2011) Testicular failure and male infertility in the monogenic Mulibrey nanism disorder.
J Clin Endocrinol Metab. 96 (11): 3399-407. -
Vanmontfort, D. et al. (1998) cDNA sequence analysis, gene expression and protein localisation of the inhibin alpha-subunit of Australian brushtail possum (Trichosurus vulpecula).
J Mol Endocrinol. 21 (2): 141-52. -
Katoh, T. et al. (2012) Estrogen-producing endometrioid adenocarcinoma resembling sex cord-stromal tumor of the ovary: a review of four postmenopausal cases.
Diagn Pathol. 7: 164. -
Biancani, B. et al. (2010) Ovarian interstitial cell tumor in a South American sea lion (Otaria flavescens).
J Wildl Dis. 46: 1012-6. -
Marino, F.E. et al. (2014) The inhibin/activin signalling pathway in human gonadal and adrenal cancers.
Mol Hum Reprod. 20 (12): 1223-37. -
Wang Y et al. (2016) Follicle Depletion Provides A Permissive Environment for Ovarian Carcinogenesis.
Mol Cell Biol. Jun 27. pii: MCB.00202-16. [Epub ahead of print] -
Canadas, A. et al. (2016) Multiple Cutaneous Metastasis of a Malignant Leydig Cell Tumour in a Dog.
J Comp Pathol. 155 (2-3): 181-4. -
Bae, G.E. et al. (2016) Placenta percreta with small bowel invasion: unusual presentation of abnormal placental adherence
Int J Clin Exp Pathol 9(7):7237-43 -
Liang, L. et al. (2016) Renal cell carcinoma metastatic to the ovary or fallopian tube: a clinicopathological study of 9 cases.
Hum Pathol. 51: 96-102. -
Stewart CJ et al. (2016) SF1 immunohistochemistry is useful in differentiating uterine tumours resembling sex cord-stromal tumours from potential histological mimics.
Pathology. 48 (5): 434-40. -
Paulini, F. et al. (2016) Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting.
Reprod Biomed Online. May 13. pii: S1472-6483(16)30294-2. [Epub ahead of print] -
Lai, J.P. et al. (2015) Isolated Large Cell Calcifying Sertoli Cell Tumor in a Young Boy, not Associated with Peutz-Jeghers Syndrome or Carney Complex.
Ann Clin Lab Res. 3 (1): 2. -
Strehl, J.D. et al. (2015) Pattern of SMARCB1 (INI1) and SMARCA4 (BRG1) in poorly differentiated endometrioid adenocarcinoma of the uterus: analysis of a series with emphasis on a novel SMARCA4-deficient dedifferentiated rhabdoid variant.
Ann Diagn Pathol. 19 (4): 198-202. -
Solomon LW & Velez I (2016) S-100 Negative Granular Cell Tumor of the Oral Cavity.
Head Neck Pathol. 10 (3): 367-73. -
Evron, S. et al. (2007) Activin betaA in term placenta and its correlation with placental inflammation in parturients having epidural or systemic meperidine analgesia: a randomized study.
J Clin Anesth. 19 (3): 168-74. -
An, S. et al. (2015) Granular cell tumor of the gastrointestinal tract: histologic and immunohistochemical analysis of 98 cases.
Hum Pathol. 46 (6): 813-9. -
Wakayama, A. et al. (2017) Recurrent female adnexal tumor of probable Wolffian origin treated with debulking surgery, imatinib and paclitaxel/carboplatin combination chemotherapy: A case report.
Oncol Lett. 13 (5): 3403-8. -
Rekhi, B. et al. (2012) Alveolar soft part sarcoma 'revisited': clinicopathological review of 47 cases from a tertiary cancer referral centre, including immunohistochemical expression of TFE3 in 22 cases and 21 other tumours.
Pathology. 44 (1): 11-7. -
Polinas, M. et al. (2016) Co-occurrence of a metastatic mammary liposarcoma and an ovarian sex-cord stromal tumor in a dog.
Res Vet Sci. 109: 157-60. -
Banco, B. et al. (2017) Ovarian Embryonal Carcinoma in a Dog.
J Comp Pathol. 157 (4): 291-5. -
Gao, Y. et al. (2017) Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling.
Reprod Biol Endocrinol. 15 (1): 94. -
Belotti, E.M. et al. (2021) Characterization of an incipient granulosa cell tumour in a Holstein cow: Steroid hormone receptors and coregulators expression.
Anat Histol Embryol. Nov 03 [Epub ahead of print].
- RRID
- AB_322161
- UniProt
- P05111
- Entrez Gene
- INHA
- GO Terms
- GO:0001501 skeletal system development
- GO:0001541 ovarian follicle development
- GO:0004871 signal transducer activity
- GO:0005125 cytokine activity
- GO:0005179 hormone activity
- GO:0006917 induction of apoptosis
- GO:0007050 cell cycle arrest
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0007267 cell-cell signaling
- View More GO Terms
- GO:0007399 nervous system development
- GO:0008083 growth factor activity
- GO:0009605 response to external stimulus
- GO:0046881 positive regulation of follicle-stimulating hormone secretion
- GO:0046882 negative regulation of follicle-stimulating hormone secretion
- GO:0030218 erythrocyte differentiation
- GO:0034673 inhibin-betaglycan-ActRII complex
- GO:0042127 regulation of cell proliferation
- GO:0042326 negative regulation of phosphorylation
- GO:0042541 hemoglobin biosynthetic process
- GO:0043512 inhibin A complex
- GO:0045077 negative regulation of interferon-gamma biosynthetic process
- GO:0045578 negative regulation of B cell differentiation
- GO:0045650 negative regulation of macrophage differentiation
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