Indoleamine 2,3-Dioxygenase antibody | 10.1

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Mouse anti Human Indoleamine 2,3-Dioxygenase

Product Type
Monoclonal Antibody
Clone
10.1
Isotype
IgG3
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT2037G C WB 0.1 mg
Mouse anti Human Indoleamine 2,3-Dioxygenase antibody, clone 10.1 recognizes Indoleamine-pyrrole 2,3 - dioxygenase (IDO), an enzyme responsible for the catabolism of tryptophan to N-formylkynurenine and kynurenine. With the exception of tryptophan oxidase in hepatocytes, IDO is the only enzyme that degrades the amino acid tryptophan to kynurenine. Chlamydial pathogens require tryptophan for growth. IDO can lower available tryptophan in target cells leading to conversion of the pathogen to persistant forms, with significance in the progression to chronic infection with its associated pathological effects.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Peptide corresponding to amino acids 78-184 of human IDO fused to GST.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P14902 Related reagents
Entrez Gene
IDO1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005829 cytosol
GO:0004833 tryptophan 2,3-dioxygenase activity
GO:0055114 oxidation-reduction process
GO:0006569 tryptophan catabolic process
GO:0007565 female pregnancy
GO:0020037 heme binding
GO:0033754 indoleamine 2,3-dioxygenase activity
Regulatory
For research purposes only

Applications of Indoleamine 2,3-Dioxygenase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
OBT2037G recognizes a 45kDa band in IFN-gamma treated human cell lines. This antibody recognizes a slightly lower molecular weight band in Mouse IDO compared to its human counterpart.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG3:FITC HCA039F 0.1 mg F
Human anti Mouse IgG3:HRP HCA039P 0.1 mg E
Human anti Mouse IgG3:RPE HCA039PE 100 Tests F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Indoleamine 2,3-Dioxygenase antibody

  1. Muller, A.J. et al. (2005) Inhibition of indoleamine 2,3-dioxygenase, an immunoregulatory target of the cancer suppression gene Bin1, potentiates cancer chemotherapy.
    Nat Med. 11 (3): 312-9.
  2. Sakash, J.B. et al. (2002) Cytokines induce indoleamine 2,3-dioxygenase expression in human atheroma-asociated cells: implications for persistent Chlamydophila pneumoniae infection.
    Infect Immun. 70 (7): 3959-61.