IL-8 antibody | 4F8A4

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Mouse anti Human Interleukin-8

Product Type
Monoclonal Antibody
Clone
4F8A4
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6192GA
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human interleukin-8 antibody, clone 4F8A4 recognizes interleukin-8 (IL-8) also known as C-X-C motif chemokine 8, emoctakin and granulocyte chemotactic protein 1. IL-8 exists as a 77 amino acid signaling protein in non-immune cells or as a 72 amino acid protein in immune cells such as monocytes and macrophages. Expression of IL-8 is primarily regulated by NF-κB, but has also been noted to be influenced by inflammatory signals, environmental stresses and steroid hormones (Brat et al. 2005).

IL-8 elicits its biological function through its G-protein coupled receptors CXCR1 and CXCR2. Upon activation of these receptors a range of signaling pathways can be initiated such as PI3K, AKT, mTOR and ARP2/3. Through this activation leukocyte function and migration can be mediated (Gonzalez-Aparicio & Alfaro 2019). IL-8 expression is unregulated in tumor cells, which thought to be a response to chemotherapeutic interventions or tumor environmental stressors such as hypoxia (Waugh & Wilson 2008).

The purified Mouse anti Human interleukin-8 antibody, clone 4F8A4 (MCA6192GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-8 antibody, clone 8C9E2 (MCA6193B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length interleukin-8 (Ser28-Ser99)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P10145
Entrez Gene
IL8
Regulatory
For research purposes only

Applications of IL-8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Interleukin-8:Biotin MCA6193B E 0.1 mg loader

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