IL-8 antibody | 4F8A4
Mouse anti Human Interleukin-8
- Product Type
- Monoclonal Antibody
- Clone
- 4F8A4
- Isotype
- IgG2a
- Specificity
- IL-8
| Mouse anti Human interleukin-8 antibody, clone 4F8A4 recognizes interleukin-8 (IL-8) also known as C-X-C motif chemokine 8, emoctakin and granulocyte chemotactic protein 1. IL-8 exists as a 77 amino acid signaling protein in non-immune cells or as a 72 amino acid protein in immune cells such as monocytes and macrophages. Expression of IL-8 is primarily regulated by NF-κB, but has also been noted to be influenced by inflammatory signals, environmental stresses and steroid hormones (Brat et al. 2005). IL-8 elicits its biological function through its G-protein coupled receptors CXCR1 and CXCR2. Upon activation of these receptors a range of signaling pathways can be initiated such as PI3K, AKT, mTOR and ARP2/3. Through this activation leukocyte function and migration can be mediated (Gonzalez-Aparicio & Alfaro 2019). IL-8 expression is unregulated in tumor cells, which thought to be a response to chemotherapeutic interventions or tumor environmental stressors such as hypoxia (Waugh & Wilson 2008). The purified Mouse anti Human interleukin-8 antibody, clone 4F8A4 (MCA6192GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-8 antibody, clone 8C9E2 (MCA6193B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human full length interleukin-8 (Ser28-Ser99)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6193B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human Interleukin-8:Biotin | MCA6193B | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Human Interleukin-8:Biotin | ||||||
MCA6192GA
100005499
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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