IL-6 antibody | B-E8

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Mouse anti Human Interleukin-6

Product Type
Monoclonal Antibody
Clone
B-E8
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA746 C* E FN* 0.5 mg
Mouse anti Human Interleukin-6 antibody, clone B-E8 recognizes human interleukin-6 (IL-6), a cytokine and potent inducer of the immune acute phase response.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant full-length human IL-6 expressed in E. coli.
Affinity
0.5nM
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells of the mouse X63 Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05231 Related reagents
Entrez Gene
IL6 Related reagents
GO Terms
GO:0001781 neutrophil apoptosis
GO:0002384 hepatic immune response
GO:0002446 neutrophil mediated immunity
GO:0002548 monocyte chemotaxis
GO:0002675 positive regulation of acute inflammatory response
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0006959 humoral immune response
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0050829 defense response to Gram-negative bacterium
GO:0008285 negative regulation of cell proliferation
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010888 negative regulation of lipid storage
GO:0045727 positive regulation of translation
GO:0030168 platelet activation
GO:0031018 endocrine pancreas development
GO:0031175 neuron projection development
GO:0032494 response to peptidoglycan
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0032966 negative regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042102 positive regulation of T cell proliferation
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0043410 positive regulation of MAPKKK cascade
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050871 positive regulation of B cell activation
GO:0051024 positive regulation of immunoglobulin secretion
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0051607 defense response to virus
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway
Regulatory
For research purposes only

Applications of IL-6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 1 20pg neutralizes activity of 1U of IL-6
Immunohistology - Frozen 2
Immunohistology - Paraffin
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for IL-6 antibody

  1. Lu, Z.Y. et al. (1993) Overall interleukin-6 production exceeds 7 mg/day in multiple myeloma complicated by sepsis.
    Cytokine 5: 578-582.
  2. Wijdenes, J. et al. (1991) Human recombinant dimeric IL-6 binds to its receptor as detected by anti IL-6 monoclonal antibodies.
    Mol. Immunol. 28: 1183 - 1192.
  3. Dechaud, H. et al. (1998) Evaluation of endometrial inflammation by quantification of macrophages, T lymphocytes and interleukin-1 and -6 in human endometrium.
    J. Assist. Reprod. Genet.15: 612 - 618.
  4. Kimoff, R.J. et al. (2011) Increased upper airway cytokines and oxidative stress in severe obstructive sleep apnoea.
    Eur Respir J. 38: 89-97.
  5. Przemioslo, R.T. et al. (1994) Raised pro-inflammatory cytokines interleukin 6 and tumour necrosis factor alpha in coeliac disease mucosa detected by immunohistochemistry.
    Gut. 35: 1398-403.
  6. Srivani, R. et al. (2003) Molecular logistics of cytokines in cervical cancer.
    Current Science. 84: 434-438.