IL-4 antibody | B04-9E1
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E ResetMouse anti Human Interleukin-4
- Product Type
- Monoclonal Antibody
- Clone
- B04-9E1
- Isotype
- IgG1
- Specificity
- IL-4
| Mouse anti Human interleukin-4 antibody, clone B04-9E1, recognizes interleukin-4 (IL-4) also known as B-cell stimulatory factor 1, binetrakin and lymphocyte stimulatory factor 1. IL-4 is a cytokine largely expressed and secreted by CD4+ T helper cells, basophils, eosinophils, mast cells and natural killer cells (Junttila 2018). IL-4 is expressed at low levels under physiological conditions, with the levels influenced by hormones, cellular/oxidative stress, infection and inflammation (McCormick & Heller 2015). IL-4 plays important roles in both physiological and pathological conditions such as Th2 cell-mediated immunity, IgE and IgG1 class switching in B cells and tissue repair and homeostasis through alternative macrophage activation (Gadani et al. 2013). The effect of IL-4 is mediated through the receptor IL-4Rα. Upon binding of IL-4 to IL-4Rα, the complex binds to a secondary receptor chain, IL-2Rγc or IL-13Rα1. This receptor complex is then able to induce intracellular signaling via JAK1 and JAK3 (Junttila 2018). The purified Mouse anti Human interleukin-4 antibody, clone B04-9E1 (MCA6240GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-4 antibody, clone B03-6A7 (MCA6241B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human full length interleukin-4 (His25-Ser153)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
-
ELISA Matched Pair-Detection Antibody:
MCA6241B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Synonyms
- BSF-1
- UniProt
- P05112
- Entrez Gene
- IL4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006935 chemotaxis
- GO:0006968 cellular defense response
- GO:0008083 growth factor activity
- GO:0008203 cholesterol metabolic process
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- View More GO Terms
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0042092 type 2 immune response
- GO:0043066 negative regulation of apoptosis
- GO:0045189 connective tissue growth factor biosynthetic process
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
MCA6240GA
100005150
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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