IL-3 antibody | 5A4-1-16
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WB ResetMouse anti Human Interleukin-3:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 5A4-1-16
- Isotype
- IgG1
- Specificity
- IL-3
Antibody Quality and Performance Guarantee
| Mouse anti Human interleukin-3 antibody, clone 5A4-1-16, recognizes interleukin-3 (IL-3) also known as hematopeietic growth factor, mast cell growth factor, multi potential colony-stimulating factor and p-cell-stimulatory factor. IL-3 is a member of the β common cytokine family which is expressed by both hematopoietic and non-hematopoeitic cells. T cells are a major expresser of IL-3 during inflammation as a result of allergic reactions, parasitic and viral infection (Borriello et al. 2019). Upon binding to its receptor IL3-Rα, the JAK and STAT signaling pathways can be activated. This is used to activate plasmacytoid dendritic cells during viral infection and the expansion of mast cells and basophils during parasitic infection (Dougan et al. 2019). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human interleukin-3
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 1 EU/μg
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
8.0 ug/ml | 4.0 ug/ml |
| Western Blotting | |
0.25 ug/ml | 0.5 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P08700
- Entrez Gene
- IL3
- GO Terms
- GO:0005125 cytokine activity
- GO:0005135 interleukin-3 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0007399 nervous system development
- GO:0008083 growth factor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- View More GO Terms
- GO:0045740 positive regulation of DNA replication
- GO:0045885 positive regulation of survival gene product expression
MCA6335Z
156489
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-3 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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