IL-3 antibody | 5A4-1-16
Mouse anti Human interleukin-3 antibody, clone 5A4-1-16, recognizes interleukin-3 (IL-3) also known as hematopeietic growth factor, mast cell growth factor, multi potential colony-stimulating factor and p-cell-stimulatory factor. IL-3 is a member of the β common cytokine family which is expressed by both hematopoietic and non-hematopoeitic cells. T cells are a major expresser of IL-3 during inflammation as a result of allergic reactions, parasitic and viral infection (Borriello et al. 2019).
Upon binding to its receptor IL3-Rα, the JAK and STAT signaling pathways can be activated. This is used to activate plasmacytoid dendritic cells during viral infection and the expansion of mast cells and basophils during parasitic infection (Dougan et al. 2019).
Upon binding to its receptor IL3-Rα, the JAK and STAT signaling pathways can be activated. This is used to activate plasmacytoid dendritic cells during viral infection and the expansion of mast cells and basophils during parasitic infection (Dougan et al. 2019).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human interleukin-3
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Endotoxin Level
- < 1 EU/μg
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08700
- Entrez Gene
- IL3
- GO Terms
- GO:0005125 cytokine activity
- GO:0005135 interleukin-3 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0007399 nervous system development
- GO:0008083 growth factor activity
- GO:0008284 positive regulation of cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0045740 positive regulation of DNA replication
- GO:0045885 positive regulation of survival gene product expression
- Regulatory
- For research purposes only
Applications of IL-3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 8.0 ug/ml | 4.0 ug/ml | |
| Western Blotting | 0.25 ug/ml | 0.5 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
