IL-27 antibody | 3F12

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Mouse anti Human INTERLEUKIN-27:Preservative Free

Product Type
Monoclonal Antibody
Clone
3F12
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA3981Z WB 0.1 mg
Mouse anti Human IL-27 antibody, clone 3F12 recognizes human interleukin-27, also known as interleukin-30 or p28. IL-27 is a 215 amino acid ~25 kDa cytokine functioning in the innate immune response, potentiating the early TH1 response to insult.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 177-244 of human IL27
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q8NEV9 Related reagents
Entrez Gene
IL27 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0042129 regulation of T cell proliferation
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045087 innate immune response
GO:0045523 interleukin-27 receptor binding
GO:0045625 regulation of T-helper 1 cell differentiation
GO:0050688 regulation of defense response to virus
Regulatory
For research purposes only

Applications of IL-27 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.