IL-12 p40 antibody | I-1A4

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Anti Human Interleukin-12 Antibody, clone I-1A4 gallery image 1 — Direct ELISA showing cross reactivity of Mouse anti Human IL-12 (**MCA1613**) with porcine IL-12. Antigen coated at 3μgml in ELISA coating buffer (BUF030) and Goat anti Mouse Ig (STAR87P) as tertiary detection reagent.

Mouse anti Human Interleukin-12

Product Type: Monoclonal Antibody
Clone: I-1A4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1613	C E			0.5 mg

Mouse anti Human Interleukin-12 antibody, clone I-1A4 recognizes the p40 subunit of human interleukin-12, and binds to both the free subunit and the p35/p40 heterodimer.

Mouse anti Human Interleukin-12 antibody, clone I-1A4 reacts with both stimulated and unstimulated monocytes in immunohistology.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Pig

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Recombinant human IL-12.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2-0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P29460 Related reagents
Entrez Gene: IL12B Related reagents
GO Terms: GO:0001916 positive regulation of T cell mediated cytotoxicity; GO:0002230 positive regulation of defense response to virus by host; GO:0002827 positive regulation of T-helper 1 type immune response; GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; GO:0016020 membrane; GO:0004896 cytokine receptor activity; GO:0005125 cytokine activity; GO:0005143 interleukin-12 receptor binding; GO:0007050 cell cycle arrest; GO:0050829 defense response to Gram-negative bacterium; GO:0010224 response to UV-B; GO:0010535 positive regulation of activation of JAK2 kinase activity; GO:0016477 cell migration; GO:0019953 sexual reproduction; GO:0032693 negative regulation of interleukin-10 production; GO:0032700 negative regulation of interleukin-17 production; GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production; GO:0032729 positive regulation of interferon-gamma production; GO:0032733 positive regulation of interleukin-10 production; GO:0032735 positive regulation of interleukin-12 production; GO:0032740 positive regulation of interleukin-17 production; GO:0032760 positive regulation of tumor necrosis factor production; GO:0032819 positive regulation of natural killer cell proliferation; GO:0034105 positive regulation of tissue remodeling; GO:0034393 positive regulation of smooth muscle cell apoptosis; GO:0042088 T-helper 1 type immune response; GO:0042093 T-helper cell differentiation; GO:0042095 interferon-gamma biosynthetic process; GO:0042104 positive regulation of activated T cell proliferation; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein; GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein; GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein; GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein; GO:0043382 positive regulation of memory T cell differentiation; GO:0043514 interleukin-12 complex; GO:0045078 positive regulation of interferon-gamma biosynthetic process; GO:0045672 positive regulation of osteoclast differentiation; GO:0045785 positive regulation of cell adhesion; GO:0046982 protein heterodimerization activity; GO:0048662 negative regulation of smooth muscle cell proliferation; GO:0050729 positive regulation of inflammatory response; GO:0051135 positive regulation of NK T cell activation; GO:0051142 positive regulation of NK T cell proliferation; GO:0070743 interleukin-23 complex
Regulatory: For research purposes only

Applications of IL-12 p40 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/1000 as detecting antibody
Immunohistology - Frozen

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for IL-12 p40 antibody

Visvanathan S et al. (2011) Tissue and serum markers of inflammation during the follow-up of patients with giant-cell arteritis--a prospective longitudinal study.
Rheumatology (Oxford). 50 (11): 2061-70.
Nagashima, N. et al. (2008) Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.
Anticancer Res. 28: 1277-83.
Carter R.W. et al. (1997) Production and characterization of monoclonal antibodies to human interleukin-12.
Hybridoma. 16: 363-9.

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