IL-12 p40 antibody | I-1A4

Mouse anti Human Interleukin-12

Product Type
Monoclonal Antibody
Clone
I-1A4
Isotype
IgG1
Specificity
IL-12 p40

Product Code Applications Pack Size List Price Your Price Qty
MCA1613
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.5 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human Interleukin-12 antibody, clone I-1A4 recognizes the p40 subunit of human interleukin-12, and binds to both the free IL-12 p40 subunit and the p35/p40 heterodimer.

Mouse anti Human Interleukin-12 antibody, clone I-1A4 reacts with both stimulated and unstimulated monocytes in immunohistology.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant human IL-12.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2-0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 as detecting antibody
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for IL-12 p40 antibody

  1. Visvanathan S et al. (2011) Tissue and serum markers of inflammation during the follow-up of patients with giant-cell arteritis--a prospective longitudinal study.
    Rheumatology (Oxford). 50 (11): 2061-70.
  2. Nagashima, N. et al. (2008) Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.
    Anticancer Res. 28: 1277-83.
  3. Carter R.W. et al. (1997) Production and characterization of monoclonal antibodies to human interleukin-12.
    Hybridoma. 16: 363-9.

RRID
AB_2123619
UniProt
P29460
Entrez Gene
IL12B
GO Terms
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0007050 cell cycle arrest
GO:0050829 defense response to Gram-negative bacterium
GO:0010224 response to UV-B
GO:0010535 positive regulation of activation of JAK2 kinase activity
GO:0016477 cell migration
GO:0019953 sexual reproduction
GO:0032693 negative regulation of interleukin-10 production
GO:0032700 negative regulation of interleukin-17 production
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032729 positive regulation of interferon-gamma production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032740 positive regulation of interleukin-17 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0032819 positive regulation of natural killer cell proliferation
GO:0034105 positive regulation of tissue remodeling
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042088 T-helper 1 type immune response
GO:0042093 T-helper cell differentiation
GO:0042095 interferon-gamma biosynthetic process
GO:0042104 positive regulation of activated T cell proliferation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0042510 regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042520 positive regulation of tyrosine phosphorylation of Stat4 protein
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043382 positive regulation of memory T cell differentiation
GO:0043514 interleukin-12 complex
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0045785 positive regulation of cell adhesion
GO:0046982 protein heterodimerization activity
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0050729 positive regulation of inflammatory response
GO:0051135 positive regulation of NK T cell activation
GO:0051142 positive regulation of NK T cell proliferation
GO:0070743 interleukin-23 complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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