HLA G antibody | MEM-G/9
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|Mouse anti Human HLA G antibody, clone MEM-G/9 recognizes human HLA-G, a non-classical major histocompatibility complex (MHC) molecule. HLA-G expression is restricted to trophoblast cells and some medullary thymic epithelial cells. Several isoforms of the HLA-G molecule exist, which include the membrane bound isoforms HLA-G1 – G4 and soluble isoforms HLA-G5 – G7. Clone MEM-G/9 specifically recognizes surface expressed native HLA-G1, when associated with beta 2 microglobulin, but not does recognize the isoforms HLA-G2, G3 and G4. CMouse anti Human HLA G antibody, clone MEM-G/9 has also been reported to recognize the soluble isoform HLA-G5.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Recombinant human HLA-G refolded with beta 2 microglobulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with myeloma cells.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for HLA G antibody
Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
Tissue Antigens. 55 (6): 510-8.
Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64 (3): 315-26.
Kotze, D.J. et al. (2010) Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome.
J Assist Reprod Genet. 27 (6): 309-16.
Guetta, E. et al (2005) Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.
J Histochem Cytochem. 53: 337-9.
Hiby, S.E. et al (2010) Maternal activating KIRs protect against human reproductive failure mediated by fetal HLA-C2
J Clin Invest. 120: 4102-10.
Sher, G. et al. (2005) Influence of early ICSI-derived embryo sHLA-G expression on pregnancy and implantation rates: a prospective study.
Hum Reprod. 20: 1359-63.
Sher, G. et al. (2005) Soluble human leukocyte antigen G expression in phase I culture media at 46 hours after fertilization predicts pregnancy and implantation from day 3 embryo transfer.
Fertil Steril. 83: 1410-3.
Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
Mol Hum Reprod. 17: 577-86.
View The Latest Product References
Manaster, I. et al. (2012) MiRNA-mediated control of HLA-G expression and function.
PLoS One. 7: e33395.
Nückel, H. et al. (2005) HLA-G expression is associated with an unfavorable outcome and immunodeficiency in chronic lymphocytic leukemia.
Blood. 105: 1694-8.
Yao, Y.Q. et al. (2005) Differential expression of alternatively spliced transcripts of HLA-G in human preimplantation embryos and inner cell masses.
J Immunol. 175 (12): 8379-85.
de Carvalho, J.F. et al. (2012) Heparin increases HLA-G levels in primary antiphospholipid syndrome.
Clin Dev Immunol. 2012: 232390.
Guenther, S. et al. (2012) Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis.
Int J Mol Sci. 13 (7): 9069-80.
Apps, R. et al. (2011) Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
Placenta. 32 (1): 33-43.
Lim DS et al. (2014) The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.
J Immunol. 193 (10): 5065-75.
Reches, A. et al. (2016) HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
J Immunol. 196 (12): 4967-76.
Bröker, P et al. (2012) A nanostructured SAW chip-based biosensor detecting cancer cells
Sensors and Actuators B: Chemical. 165 (1): 1-6.
Reches, A. et al. (2020) A Unique Regulation Region in the 3' UTR of HLA-G with a Promising Potential.
Int J Mol Sci. 21 (3): 900.
- Entrez Gene
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0032393 MHC class I receptor activity
- GO:0050776 regulation of immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- View More GO Terms
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