HLA G antibody | MEM-G/9

Mouse anti Human HLA G

Product Type
Monoclonal Antibody
Clone
MEM-G/9
Isotype
IgG1
Specificity
HLA G

Product Code Applications Pack Size List Price Your Price Qty
MCA2044
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IF 0.2 mg loader
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loader
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Mouse anti Human HLA G antibody, clone MEM-G/9 recognizes human HLA-G, a non-classical major histocompatibility complex (MHC) molecule. HLA-G expression is restricted to trophoblast cells and some medullary thymic epithelial cells. Several isoforms of the HLA-G molecule exist, which include the membrane bound isoforms HLA-G1 – G4 and soluble isoforms HLA-G5 – G7. Clone MEM-G/9 specifically recognizes surface expressed native HLA-G1, when associated with beta 2 microglobulin, but not does recognize the isoforms HLA-G2, G3 and G4. CMouse anti Human HLA G antibody, clone MEM-G/9 has also been reported to recognize the soluble isoform HLA-G5.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Recombinant human HLA-G refolded with beta 2 microglobulin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with myeloma cells.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for HLA G antibody

  1. Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
    Tissue Antigens. 55 (6): 510-8.
  2. Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
    Hum Immunol. 64 (3): 315-26.
  3. Kotze, D.J. et al. (2010) Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome.
    J Assist Reprod Genet. 27 (6): 309-16.
  4. Guetta, E. et al (2005) Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.
    J Histochem Cytochem. 53: 337-9.
  5. Hiby, S.E. et al (2010) Maternal activating KIRs protect against human reproductive failure mediated by fetal HLA-C2
    J Clin Invest. 120: 4102-10.
  6. Sher, G. et al. (2005) Influence of early ICSI-derived embryo sHLA-G expression on pregnancy and implantation rates: a prospective study.
    Hum Reprod. 20: 1359-63.
  7. Sher, G. et al. (2005) Soluble human leukocyte antigen G expression in phase I culture media at 46 hours after fertilization predicts pregnancy and implantation from day 3 embryo transfer.
    Fertil Steril. 83: 1410-3.
  8. Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
    Mol Hum Reprod. 17: 577-86.
  9. View The Latest Product References
  10. Manaster, I. et al. (2012) MiRNA-mediated control of HLA-G expression and function.
    PLoS One. 7: e33395.
  11. Nückel, H. et al. (2005) HLA-G expression is associated with an unfavorable outcome and immunodeficiency in chronic lymphocytic leukemia.
    Blood. 105: 1694-8.
  12. Yao, Y.Q. et al. (2005) Differential expression of alternatively spliced transcripts of HLA-G in human preimplantation embryos and inner cell masses.
    J Immunol. 175 (12): 8379-85.
  13. de Carvalho, J.F. et al. (2012) Heparin increases HLA-G levels in primary antiphospholipid syndrome.
    Clin Dev Immunol. 2012: 232390.
  14. Guenther, S. et al. (2012) Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis.
    Int J Mol Sci. 13 (7): 9069-80.
  15. Apps, R. et al. (2011) Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
    Placenta. 32 (1): 33-43.
  16. Lim DS et al. (2014) The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.
    J Immunol. 193 (10): 5065-75.
  17. Reches, A. et al. (2016) HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
    J Immunol. 196 (12): 4967-76.
  18. Bröker, P et al. (2012) A nanostructured SAW chip-based biosensor detecting cancer cells
    Sensors and Actuators B: Chemical. 165 (1): 1-6.
  19. Reches, A. et al. (2020) A Unique Regulation Region in the 3' UTR of HLA-G with a Promising Potential.
    Int J Mol Sci. 21 (3): 900.

Flow Cytometry

Immunofluorescence

RRID
AB_323364
UniProt
P17693
Entrez Gene
HLA-G
GO Terms
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0032393 MHC class I receptor activity
GO:0050776 regulation of immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
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