HLA G antibody | MEM-G/9
Mouse anti Human HLA-G antibody, clone MEM-G/9 (MCA2044) used for the evaluation of HLA-G expression on transfected cells by flow cytometry.
Image caption:
MiR-148a and miR-152 down-regulate HLA-G expression and reduce LILRB1 binding. 721.221/HLA-G (G variant, A, or C variant, B) were transduced with the control miRNA, miR-148a or miR-152. The various cells were then stained with anti HLA-G mAb and analyzed by FACS. One out of three representative experiments is shown. (C) FACS histograms re HLA-G staining of 721.221/HLA-G cells not expressing the 3′UTR of HLA-G. One out of three representative experiments is shown. (D–E) LILRB1-Ig staining of 721.221/HLA-G (G variant, D, or C variant, E) and of 21.221/HLA-G cells not expressing the 3′UTR of HLA-G (F) transduced with control miRNA, with miR-148a or with miR-152. Black histogram: cells transduced with a control miRNA. Dark grey histogram: cells transduced with miR-148a. Light grey histogram: cells transduced with miR-152. One out of three representative experiments is shown. (G) Quantitative real-time PCR analysis of HLA-G mRNA levels in 721.221/HLA-G cells (G variant), presented relative to hUBC. One out of three representative experiments is shown.
From: Manaster I, Goldman-Wohl D, Greenfield C, Nachmani D, Tsukerman P, Hamani Y, et al. (2012)
MiRNA-Mediated Control of HLA-G Expression and Function.
PLoS ONE 7(3): e33395.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti HLA-G antibody, clone MEM-G/9 (MCA2044) used for the evaluation of cuirculating HLA-G in Primary Antiphospholipid Syndrome and control patients by ELISA.
Image caption:
HLA-G serum levels in 44 PAPS patients and 43 healthy controls, and in PAPS patients with and without heparin.
From: de Carvalho JF, de Oliveira RM, Rodrigues CE, Glezer A, Bonfá E, Pereira RM.
Heparin increases HLA-G levels in primary antiphospholipid syndrome.
Clin Dev Immunol. 2012;2012:232390.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Human HLA-G antibody, clone MEM-G/9 (MCA2044F) used for the demonstration of HLA-G expression on placental trophoblasts by immunofluorescence
Image caption:
Dual immunofluorescence using HLA-G (an extravillous trophoblast marker) and anti-M30 cytodeath reveals that trophoblast apoptosis is markedly increased in spontaneous abortions.
From: Guenther S, Vrekoussis T, Heublein S, Bayer B, Anz D, Knabl J, Navrozoglou I, Dian D, Friese K, Makrigiannakis A, Jeschke U.
Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis.
Int J Mol Sci. 2012;13(7):9069-80.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Human HLA-G antibody, clone MEM-G/9 (MCA2044F) used for the identification of HLA-G expressing trophoblast cells by flow cytometry.
Image caption:
The IL-2 receptor β subunit is up-regulated on trophoblast cells as they differentiate from villous to extravillous phenotype. Upregulation of IL-2Rβ expression was validated by flow cytometry (A–C) and immunohistochemistry (D). Preparations of placental cells from normal first trimester pregnancies were gated on scatter, leukocytes excluded by CD45 labelling (gate R2) and subunits of the IL-15 receptor complex stained on villous (EGF-R+) or extravillous (HLA-G+) trophoblast cells (A). Dot plots are shown of IL-2Rβ subunit staining villous (B) and extravillous trophoblast (C). Shown here are cells from the same placenta which is representative of 3 pregnancies analysed. The IL-2Rβ was also localised histologically in sections of implantation site from the first trimester (D). The low power plan stained with cytokeratin shows villous mesenchyme (top left) and a column of EVT (bottom right) developing from placental villi (centre). Higher power pictures show strong IL-2Rβ staining detected on extravillous trophoblast migrating away from the villi, whereas staining was negligible on villous trophoblast and villous mesenchymal cells.
From: Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A. Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
Placenta. 2011 Jan;32(1):33-43.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human HLA G antibody, clone MEM-G/9 (MCA2044) used to label HLA-G expressing cells in extravillous tissue by immunofluorescence.
Image caption:
Phenotyping of extravillous tissue by double immunofluorescence staining. a, b Triple filter excitation showing both CK7 and HLA-G. b, c CK7, bound by Cy-3-labelled secondary antibody, stained red, marking extravillous trophoblasts (EVTs) and syncytiotrophoblast (SCN) together. e, f HLA-G, bound by Cy-2-labelled secondary antibody, stained green, marking the EVT. HLA-G labelling can be seen to be reduced in the GDM group (a) compared to the control (b) in EVT. Arrowheads indicate HLA-G-expressing cells Scale bar: 100 μm. GDM Gestational diabetes mellitus
From: Knabl J, Hüttenbrenner R, Mahner S, Kainer F, Desoye G, Jeschke U.
Lower HLA-G levels in extravillous trophoblasts of human term placenta in gestational diabetes mellitus than in normal controls.
Histochem Cell Biol. 2023 Jun;159(6):527-35.
doi: 10.1007/s00418-022-02163-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F E IF ResetMouse anti Human HLA G
- Product Type
- Monoclonal Antibody
- Clone
- MEM-G/9
- Isotype
- IgG1
- Specificity
- HLA G
Antibody Quality and Performance Guarantee
| Mouse anti Human HLA G antibody, clone MEM-G/9 recognizes human HLA-G, a non-classical major histocompatibility complex (MHC) molecule. HLA-G expression is restricted to trophoblast cells and some medullary thymic epithelial cells. Several isoforms of the HLA-G molecule exist, which include the membrane bound isoforms HLA-G1 – G4 and soluble isoforms HLA-G5 – G7. Mouse anti Human HLA G antibody, clone MEM-G/9 recognizes surface expressed native HLA-G1, when associated with beta 2 microglobulin, but not does recognize the isoforms HLA-G2, G3 and G4. Mouse anti Human HLA G antibody, clone MEM-G/9 has also been reported to recognize the soluble isoform HLA-G5. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Recombinant human HLA-G refolded with beta 2 microglobulin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with myeloma cells.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunofluorescence | |
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| Immunohistology - Frozen 1 | |
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| Immunohistology - Paraffin | |||
| Western Blotting |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for HLA G antibody
-
Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
Tissue Antigens. 55 (6): 510-8. -
Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64 (3): 315-26. -
Kotze, D.J. et al. (2010) Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome.
J Assist Reprod Genet. 27 (6): 309-16. -
Guetta, E. et al (2005) Trophoblasts isolated from the maternal circulation: in vitro expansion and potential application in non-invasive prenatal diagnosis.
J Histochem Cytochem. 53: 337-9. -
Hiby, S.E. et al (2010) Maternal activating KIRs protect against human reproductive failure mediated by fetal HLA-C2
J Clin Invest. 120: 4102-10. -
Sher, G. et al. (2005) Influence of early ICSI-derived embryo sHLA-G expression on pregnancy and implantation rates: a prospective study.
Hum Reprod. 20: 1359-63. -
Sher, G. et al. (2005) Soluble human leukocyte antigen G expression in phase I culture media at 46 hours after fertilization predicts pregnancy and implantation from day 3 embryo transfer.
Fertil Steril. 83: 1410-3. -
Apps, R. et al. (2011) Ex vivo functional responses to HLA-G differ between blood and decidual NK cells.
Mol Hum Reprod. 17: 577-86.
View The Latest Product References
-
Manaster, I. et al. (2012) MiRNA-mediated control of HLA-G expression and function.
PLoS One. 7: e33395. -
Nückel, H. et al. (2005) HLA-G expression is associated with an unfavorable outcome and immunodeficiency in chronic lymphocytic leukemia.
Blood. 105: 1694-8. -
Yao, Y.Q. et al. (2005) Differential expression of alternatively spliced transcripts of HLA-G in human preimplantation embryos and inner cell masses.
J Immunol. 175 (12): 8379-85. -
de Carvalho, J.F. et al. (2012) Heparin increases HLA-G levels in primary antiphospholipid syndrome.
Clin Dev Immunol. 2012: 232390. -
Guenther, S. et al. (2012) Decidual macrophages are significantly increased in spontaneous miscarriages and over-express FasL: a potential role for macrophages in trophoblast apoptosis.
Int J Mol Sci. 13 (7): 9069-80. -
Apps, R. et al. (2011) Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
Placenta. 32 (1): 33-43. -
Lim DS et al. (2014) The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.
J Immunol. 193 (10): 5065-75. -
Reches, A. et al. (2016) HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.
J Immunol. 196 (12): 4967-76. -
Bröker, P et al. (2012) A nanostructured SAW chip-based biosensor detecting cancer cells
Sensors and Actuators B: Chemical. 165 (1): 1-6. -
Reches, A. et al. (2020) A Unique Regulation Region in the 3' UTR of HLA-G with a Promising Potential.
Int J Mol Sci. 21 (3): 900. -
Gamliel, M. et al. (2018) Trained Memory of Human Uterine NK Cells Enhances Their Function in Subsequent Pregnancies.
Immunity. 48 (5): 951-962.e5. -
Stavrou, S. et al. (2018) TAAR1 induces a disturbed GSK3β phosphorylation in recurrent miscarriages through the ODC.
Endocr Connect. 7 (2): 372-84. -
Knabl, J. et al. (2023) Lower HLA-G levels in extravillous trophoblasts of human term placenta in gestational diabetes mellitus than in normal controls.
Histochem Cell Biol. 159 (6): 527-35.
- RRID
- AB_323364
- UniProt
- P17693
- Entrez Gene
- HLA-G
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0032393 MHC class I receptor activity
- GO:0050776 regulation of immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- View More GO Terms
MCA2044
153575
162097
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