HLA G antibody | MEM-G/1
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Mouse anti Human HLA G antibody, clone MEM-G/1 reacts with denatured soluble and membrane-bound HLA G heavy chain.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region.
HLA G belongs to the HLA class I molecules (HLA Class Ib; nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region.
HLA G belongs to the HLA class I molecules (HLA Class Ib; nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Denatured bacterially expressed recombinant human HLA G heavy chain.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P17693 Related reagents
- Entrez Gene
- HLA-G Related reagents
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0032393 MHC class I receptor activity
- GO:0050776 regulation of immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of HLA G antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 1/60 | 1/100 | |
| Western Blotting 2 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2MEM-G/1 recognizes HLA G under reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human placenta - extravillous cytotrophoblast
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Flow Cytometry
Functional Assays
Immunofluorescence
Western Blotting
Product Specific References
References for HLA G antibody
-
Frumento, G. et al. (2000) Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment.
Tissue Antigens. 56 (1): 30-7. -
Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
Tissue Antigens. 55 (6): 510-8. -
Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64 (3): 315-26. -
Orozco, A.F. and Lewis, D.E. (2010) Flow cytometric analysis of circulating microparticles in plasma.
Cytometry A. 77: 502-14. -
Comiskey, M. et al. (2007) HLA-G is found in lipid rafts and can act as a signaling molecule.
Hum Immunol. 68: 1-11. -
Chang, C.T. et al. (2005) Immunophenotypic and molecular cytogenetic features of the cell line UP-LN1 established from a lymph node metastasis of a poorly-differentiated carcinoma.
Anticancer Res. 25: 683-91.
