HLA G antibody | MEM-G/1

Mouse anti Human HLA G

Product Type
Monoclonal Antibody
Clone
MEM-G/1
Isotype
IgG1
Specificity
HLA G

Product Code Applications Pack Size List Price Your Price Qty
MCA2043
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SDS Safety Datasheet SDS
P * WB * 0.2 mg loader
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loader
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Mouse anti Human HLA G antibody, clone MEM-G/1 reacts with denatured soluble and membrane-bound HLA G heavy chain.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region.

HLA G belongs to the HLA class I molecules (HLA Class Ib; nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Denatured bacterially expressed recombinant human HLA G heavy chain.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/60 1/100
Western Blotting 2
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  2. 2MEM-G/1 recognizes HLA G under reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human placenta - extravillous cytotrophoblast

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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References for HLA G antibody

  1. Frumento, G. et al. (2000) Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment.
    Tissue Antigens. 56 (1): 30-7.
  2. Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
    Tissue Antigens. 55 (6): 510-8.
  3. Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
    Hum Immunol. 64 (3): 315-26.
  4. Orozco, A.F. and Lewis, D.E. (2010) Flow cytometric analysis of circulating microparticles in plasma.
    Cytometry A. 77: 502-14.
  5. Comiskey, M. et al. (2007) HLA-G is found in lipid rafts and can act as a signaling molecule.
    Hum Immunol. 68: 1-11.
  6. Chang, C.T. et al. (2005) Immunophenotypic and molecular cytogenetic features of the cell line UP-LN1 established from a lymph node metastasis of a poorly-differentiated carcinoma.
    Anticancer Res. 25: 683-91.

Flow Cytometry

Functional Assays

Immunofluorescence

Western Blotting

RRID
AB_323365
UniProt
P17693
Entrez Gene
HLA-G
GO Terms
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0032393 MHC class I receptor activity
GO:0050776 regulation of immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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