HLA G antibody | MEM-G/1
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region.
HLA G belongs to the HLA class I molecules (HLA Class Ib; nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Denatured bacterially expressed recombinant human HLA G heavy chain.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0032393 MHC class I receptor activity
- GO:0050776 regulation of immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- For research purposes only
Applications of HLA G antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/60||1/100|
|Western Blotting 2|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2MEM-G/1 recognizes HLA G under reducing conditions
- Histology Positive Control Tissue
- Human placenta - extravillous cytotrophoblast
Secondary Antibodies Available
Application Based External Images
Product Specific References
References for HLA G antibody
Frumento, G. et al. (2000) Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment.
Tissue Antigens. 56 (1): 30-7.
Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
Tissue Antigens. 55 (6): 510-8.
Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64 (3): 315-26.
Orozco, A.F. and Lewis, D.E. (2010) Flow cytometric analysis of circulating microparticles in plasma.
Cytometry A. 77: 502-14.
Comiskey, M. et al. (2007) HLA-G is found in lipid rafts and can act as a signaling molecule.
Hum Immunol. 68: 1-11.
Chang, C.T. et al. (2005) Immunophenotypic and molecular cytogenetic features of the cell line UP-LN1 established from a lymph node metastasis of a poorly-differentiated carcinoma.
Anticancer Res. 25: 683-91.