HLA G antibody | MEM-G/1
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Mouse anti Human HLA G
- Product Type
- Monoclonal Antibody
- Clone
- MEM-G/1
- Isotype
- IgG1
- Specificity
- HLA G
Antibody Quality and Performance Guarantee
| Mouse anti Human HLA G antibody, clone MEM-G/1 reacts with denatured soluble and membrane-bound HLA G heavy chain. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. HLA G belongs to the HLA class I molecules (HLA Class Ib; nonclassical) and is involved in the presentation of foreign antigens to the immune system. This molecule is expressed on the surface of trophoblast cells and plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Denatured bacterially expressed recombinant human HLA G heavy chain.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
1/60 | 1/100 |
| Western Blotting 2 | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2MEM-G/1 recognizes HLA G under reducing conditions
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human placenta - extravillous cytotrophoblast
References for HLA G antibody
-
Frumento, G. et al. (2000) Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment.
Tissue Antigens. 56 (1): 30-7. -
Fournel, S. et al. (2000) Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules.
Tissue Antigens. 55 (6): 510-8. -
Menier, C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64 (3): 315-26. -
Chang, C.T. et al. (2005) Immunophenotypic and molecular cytogenetic features of the cell line UP-LN1 established from a lymph node metastasis of a poorly-differentiated carcinoma.
Anticancer Res. 25: 683-91. -
Comiskey, M. et al. (2007) HLA-G is found in lipid rafts and can act as a signaling molecule.
Hum Immunol. 68: 1-11. -
Sun, L.L. et al. (2008) Down-regulation of HLA-G boosted natural killer cell-mediated cytolysis in JEG-3 cells cultured in vitro.
Fertil Steril. 90 (6): 2398-405. -
Orozco, A.F. and Lewis, D.E. (2010) Flow cytometric analysis of circulating microparticles in plasma.
Cytometry A. 77: 502-14. -
Lash, G.E. et al. (2010) Regulation of extravillous trophoblast invasion by uterine natural killer cells is dependent on gestational age.
Hum Reprod. 25 (5): 1137-45.
View The Latest Product References
-
Zhu, X. et al. (2012) Expression of human leukocyte antigen-G during normal placentation and in preeclamptic pregnancies.
Hypertens Pregnancy. 31 (2): 252-60. -
Schmitt, J. et al. (2015) Human Parthenogenetic Embryonic Stem Cell-Derived Neural Stem Cells Express HLA-G and Show Unique Resistance to NK Cell-Mediated Killing.
Mol Med. 21 (1): 185-96. -
Vilsmaier, T. et al. (2021) The decidual expression of Interleukin-7 is upregulated in early pregnancy loss.
Am J Reprod Immunol. 86 (3): e13437. -
Knabl, J. et al. (2023) Lower HLA-G levels in extravillous trophoblasts of human term placenta in gestational diabetes mellitus than in normal controls.
Histochem Cell Biol. 159 (6): 527-35.
- RRID
- AB_323365
- UniProt
- P17693
- Entrez Gene
- HLA-G
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0032393 MHC class I receptor activity
- GO:0050776 regulation of immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
- View More GO Terms
MCA2043
147885
153574
162785
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