HLA E antibody | MEM-E/02

Mouse anti Human HLA E

Product Type
Monoclonal Antibody
Clone
MEM-E/02
Isotype
IgG1
Specificity
HLA E

Product Code Applications Pack Size List Price Your Price Qty
MCA2193
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human HLA-E antibody, clone MEM-E/02 reacts with the denatured heavy chain of the non-classical MHC class I HLA-E molecule. HLA-E exists as a heterodimer consisting of a heavy chain and a light chain, beta-2-microglobulin.

Mouse anti Human HLA-E antibody, clone MEM-E/02 does not recognize native HLA-E by flow cytometry and does not cross react with HLA-A, -B, -C or G.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant HLA-E.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate positive/negative controls.
Western Blotting
MCA2193 detects a band of approximately 43kDa.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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loader
Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for HLA E antibody

  1. Cui, C.H. et al. (2011) Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy.
    Hum Mol Genet. 20 (2): 235-44.
  2. Derré, L. et al. (2006) Expression and release of HLA-E by melanoma cells and melanocytes: potential impact on the response of cytotoxic effector cells.
    J Immunol. 177: 3100-7.
  3. Menier; C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
    Hum Immunol. 64: 315-26.
  4. Coupel, S. et al. (2007) Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Blood. 109: 2806-14.
  5. Ashrafi, G.H. et al. (2005) E5 protein of human papillomavirus type 16 selectively downregulates surface HLA class I.
    Int J Cancer. 113: 276-83.
  6. Trichet, V. et al. (2006) Complex interplay of activating and inhibitory signals received by Vgamma9Vdelta2 T cells revealed by target cell beta2-microglobulin knockdown.
    J Immunol. 177: 6129-36.
  7. Griffin, C. et al. (2005) Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18.
    J Gen Virol. 86: 2999-3008.
  8. Djajadiningrat, R.S. et al. (2015) Classic and nonclassic HLA class I expression in penile cancer and relation to HPV status and clinical outcome.
    J Urol. 193 (4): 1245-51.
  9. View The Latest Product References
  10. Eugène, J. et al. (2019) The inhibitory receptor CD94/NKG2A on CD8+ tumor-infiltrating lymphocytes in colorectal cancer: a promising new druggable immune checkpoint in the context of HLAE/β2m overexpression.
    Mod Pathol. Aug 13 [Epub ahead of print].

Immunofluorescence

Western Blotting

RRID
AB_324025
UniProt
P13747
Entrez Gene
HLA-E
GO Terms
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0032393 MHC class I receptor activity
GO:0050776 regulation of immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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