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HLA E antibody | MEM-E/02

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Anti Human HLA E Antibody, clone MEM-E/02 gallery image 1
Western blot analysis of A431 human epidermoid carcinoma whole cell lysate probed with Mouse anti Human HLA E antibody (MCA2193) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

Mouse anti Human HLA E

Product Type
Monoclonal Antibody
Clone
MEM-E/02
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2193
Datasheet
WB
MSDS
0.2 mg loader

Mouse anti Human HLA-E antibody, clone MEM-E/02 reacts with the denatured heavy chain of the non-classical MHC class I HLA-E molecule. HLA-E exists as a heterodimer consisting of a heavy chain and a light chain, beta-2-microglobulin.

Mouse anti Human HLA-E antibody, clone MEM-E/02 does not recognize native HLA-E by flow cytometry and does not cross react with HLA-A, -B, -C or G.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant HLA-E.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P13747
Entrez Gene
HLA-E
GO Terms
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
GO:0042612 MHC class I protein complex
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0032393 MHC class I receptor activity
GO:0050776 regulation of immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060337 type I interferon-mediated signaling pathway
Regulatory
For research purposes only

Applications of HLA E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate positive/negative controls.
Western Blotting
MCA2193 detects a band of approximately 43kDa.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for HLA E antibody

  1. Cui, C.H. et al. (2011) Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy.
    Hum Mol Genet. 20 (2): 235-44.
  2. Derré, L. et al. (2006) Expression and release of HLA-E by melanoma cells and melanocytes: potential impact on the response of cytotoxic effector cells.
    J Immunol. 177: 3100-7.
  3. Menier; C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
    Hum Immunol. 64: 315-26.
  4. Coupel, S. et al. (2007) Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Blood. 109: 2806-14.
  5. Ashrafi, G.H. et al. (2005) E5 protein of human papillomavirus type 16 selectively downregulates surface HLA class I.
    Int J Cancer. 113: 276-83.
  6. Trichet, V. et al. (2006) Complex interplay of activating and inhibitory signals received by Vgamma9Vdelta2 T cells revealed by target cell beta2-microglobulin knockdown.
    J Immunol. 177: 6129-36.
  7. Griffin, C. et al. (2005) Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18.
    J Gen Virol. 86: 2999-3008.
  8. Djajadiningrat, R.S. et al. (2015) Classic and nonclassic HLA class I expression in penile cancer and relation to HPV status and clinical outcome.
    J Urol. 193 (4): 1245-51.
  9. Eugène, J. et al. (2019) The inhibitory receptor CD94/NKG2A on CD8+ tumor-infiltrating lymphocytes in colorectal cancer: a promising new druggable immune checkpoint in the context of HLAE/β2m overexpression.
    Mod Pathol. Aug 13 [Epub ahead of print].
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