HLA E antibody | MEM-E/02
Mouse anti Human HLA-E antibody, clone MEM-E/02 (MCA2193) used to analyze HLA expression in colorectal cances biopsies by immunohistochemistry on formalin fixed, paraffin embeddted sections.
Image caption:
Expression profile of HLA-E in primary and metastatic tumors, and its association with intraepithelial CD94+ lymphocytes. Two representative cases of mCRC with distinct HLA-E overexpression profile. (A, B) A discordant profile of HLA-E/β2m overexpression between primary tumor (negative) and its matched metastasis (positive) and (C, D) a concordant case featuring HLA-E overexpression both in primary tumor and its paired metastasis. (E) CD94+ intraepithelial lymphocytes. (F) The density of CD94+ intraepithelial lymphocytes is quite similar between primary tumors and metastases (p = 0.1 Mann Whitney test).
From: Denis Musquer M, Jouand N, Pere M, Lamer JE, Bézieau S, Matysiak T, Faroux R, Caroli Bosc FX, Rousselet MC, Leclair F, Mosnier JF, Toquet C, Gervois N, Bossard C.
High-Density of FcγRIIIA+ (CD16+) Tumor-Associated Neutrophils in Metastases Improves the Therapeutic Response of Cetuximab in Metastatic Colorectal Cancer Patients, Independently of the HLA-E/CD94-NKG2A Axis.
Front Oncol. 2021 Jun 15;11:684478.
doi: 10.3389/fonc.2021.684478.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human HLA E
- Product Type
- Monoclonal Antibody
- Clone
- MEM-E/02
- Isotype
- IgG1
- Specificity
- HLA E
Antibody Quality and Performance Guarantee
| Mouse anti Human HLA-E antibody, clone MEM-E/02 reacts with the denatured heavy chain of the non-classical MHC class I HLA-E molecule. HLA-E exists as a heterodimer consisting of a heavy chain and a light chain, beta-2-microglobulin. Mouse anti Human HLA-E antibody, clone MEM-E/02 does not recognize native HLA-E by flow cytometry and does not cross react with HLA-A, -B, -C or G. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Recombinant HLA-E.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Western Blotting |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti Human HLA E antibody, clone MEM-E/02 detects a band of approximately 43 kDa.
References for HLA E antibody
-
Menier; C. et al. (2003) Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Hum Immunol. 64: 315-26. -
Ashrafi, G.H. et al. (2005) E5 protein of human papillomavirus type 16 selectively downregulates surface HLA class I.
Int J Cancer. 113: 276-83. -
Griffin, C. et al. (2005) Characterization of a highly glycosylated form of the human cytomegalovirus HLA class I homologue gpUL18.
J Gen Virol. 86: 2999-3008. -
Derré, L. et al. (2006) Expression and release of HLA-E by melanoma cells and melanocytes: potential impact on the response of cytotoxic effector cells.
J Immunol. 177: 3100-7. -
Trichet, V. et al. (2006) Complex interplay of activating and inhibitory signals received by Vgamma9Vdelta2 T cells revealed by target cell beta2-microglobulin knockdown.
J Immunol. 177: 6129-36. -
Coupel, S. et al. (2007) Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
Blood. 109: 2806-14. -
Cui, C.H. et al. (2011) Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy.
Hum Mol Genet. 20 (2): 235-44. -
Djajadiningrat, R.S. et al. (2015) Classic and nonclassic HLA class I expression in penile cancer and relation to HPV status and clinical outcome.
J Urol. 193 (4): 1245-51.
View The Latest Product References
-
Eugène, J. et al. (2020) The inhibitory receptor CD94/NKG2A on CD8(+) tumor-infiltrating lymphocytes in colorectal cancer: a promising new druggable immune checkpoint in the context of HLAE/β2m overexpression.
Mod Pathol. 33 (3): 468-482. -
Denis Musquer, M. et al. (2021) High-Density of FcγRIIIA+ (CD16+) Tumor-Associated Neutrophils in Metastases Improves the Therapeutic Response of Cetuximab in Metastatic Colorectal Cancer Patients, Independently of the HLA-E/CD94-NKG2A Axis.
Front Oncol. 11: 684478.
- RRID
- AB_324025
- UniProt
- P13747
- Entrez Gene
- HLA-E
- GO Terms
- GO:0002474 antigen processing and presentation of peptide antigen via MHC class I
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0032393 MHC class I receptor activity
- GO:0050776 regulation of immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060337 type I interferon-mediated signaling pathway
MCA2193
150101
164868
170511
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