HLA DR antibody | HL-39

Mouse anti Human HLA DR:RPE

Product Type
Monoclonal Antibody
Clone
HL-39
Isotype
IgG3
Specificity
HLA DR

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MCA1879PE
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Mouse anti Human HLA DR antibody, clone HL-39 recognizes HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.

Mouse anti Human HLA DR antibody, clone HL-39 recognizes a determinant that is dependant upon the association of both the alpha and beta chains of HLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by hydrophobic charge induction chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Raji Burkitt lymphoma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for HLA DR antibody

  1. Horejsí, V. et al. (1986) Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.
    Tissue Antigens. 28 (5): 288-97.
  2. Philippidis, P. et al. (2010) Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery.
    Eur J Cardiothorac Surg. 37: 1360-6.

RRID
AB_323336
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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