HLA DR antibody | HL-39

Mouse anti Human HLA DR

Product Type
Monoclonal Antibody
Clone
HL-39
Isotype
IgG3
Specificity
HLA DR

Product Code Applications Pack Size List Price Your Price Qty
MCA1879
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human HLA DR antibody, clone HL-39 recognizes HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.

Mouse anti Human HLA DR antibody, clone HL-39 recognizes a determinant that is dependant upon the association of both the alpha and beta chains of HLA DR.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Raji Burkitt lymphoma cell line.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

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References for HLA DR antibody

  1. Horejsí, V. et al. (1986) Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.
    Tissue Antigens. 28 (5): 288-97.
  2. Philippidis, P. et al. (2010) Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery.
    Eur J Cardiothorac Surg. 37: 1360-6.

RRID
AB_323373
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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