HLA DP DQ DR antibody | Bu26

Mouse anti Human HLA DP DQ DR

Product Type
Monoclonal Antibody
Clone
Bu26
Isotype
IgG1
Specificity
HLA DP DQ DR

Product Code Applications Pack Size List Price Your Price Qty
MCA2497
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human HLA DP DQ DR antibody, clone Bu26 reacts with DP, DQ and DR beta chains, which are expressed by antigen presenting cells, B cells, monocytes and activated T lymphocytes.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for HLA DP DQ DR antibody

  1. Chapple, M.R. et al. (1990) A phenotypic study of B lymphocyte subpopulations in human bone marrow.
    Clin Exp Immunol. 81 (1): 166-72.
  2. Forte M et al. (1992) T-lymphocyte responses to Pneumocystis carinii in healthy and HIV-positive individuals.
    J Acquir Immune Defic Syndr. 5 (4): 409-16.
  3. Knox, P.G. & Young, L.S. (1995) Epstein-Barr virus infection of CR2-transfected epithelial cells reveals the presence of MHC class II on the virion.
    Virology. 213 (1): 147-57.
  4. Hassell AB et al. (1992) MHC restriction of synovial fluid lymphocyte responses to the triggering organism in reactive arthritis. Absence of a class I-restricted response.
    Clin Exp Immunol. 88 (3): 442-7.
  5. Kumararatne, D.S. et al. (1990) Specific lysis of mycobacterial antigen-bearing macrophages by class II MHC-restricted polyclonal T cell lines in healthy donors or patients with tuberculosis.
    Clin Exp Immunol. 80 (3): 314-23.
  6. Shokri, F. et al. (1993) Immunophenotypic and idiotypic characterisation of the leukaemic B-cells from patients with prolymphocytic leukaemia: evidence for a selective expression of immunoglobulin variable region (IGV) gene products.
    Leuk Res. 17 (8): 669-76.
  7. Pithie AD et al. (1992) Generation of cytolytic T cells in individuals infected by Mycobacterium tuberculosis and vaccinated with BCG.
    Thorax. 47 (9): 695-701.

RRID
AB_906085
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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