Histone H1 antibody | AE-4
Mouse anti Histone H1 antibody, clone AE-4 (4974-7808) used to assess H1 expression in lysates from AML patients by western blotting.
Image caption:
Characterization and quality control of human CD34+ cells and patient AML blast samples.
Examples of fractionated protein purity and quality. Nitrocellulose immunoblots of samples fractioned for nuclear (N), or cytoplasmic (C) proteins. Purity of the fractioned samples was assessed by immunoblotting for GAPDH and histone protein expression.
From: Alanazi B, Munje CR, Rastogi N, Williamson AJK, Taylor S, Hole PS, Hodges M, Doyle M, Baker S, Gilkes AF, Knapper S, Pierce A, Whetton AD, Darley RL, Tonks A.
Integrated nuclear proteomics and transcriptomics identifies S100A4 as a therapeutic target in acute myeloid leukemia.
Leukemia. 2019 Oct 14. [Epub ahead of print].
doi:10.1038/s41375-019-0596-4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Histone H1
- Product Type
- Monoclonal Antibody
- Clone
- AE-4
- Isotype
- IgG2a
- Specificity
- Histone H1
Antibody Quality and Performance Guarantee
| Mouse anti Human Histone H1 antibody, clone AE-4 recognizes histone H1. The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. In blots of rat brain homogenate a duplet is observed at ~27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Nuclei from myeloid leukamia biopsy cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
1/100 |
- 1 Acetone or PFA fixation is recommended
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil
References for Histone H1 antibody
-
Swindle, C.S. & Engler, J.A. (1998) Association of the human papillomavirus type 11 E1 protein with histone H1.
J Virol. 72 (3): 1994-2001. -
Hole, P.S. et al. (2010) Ras-induced reactive oxygen species promote growth factor-independent proliferation in human CD34+ hematopoietic progenitor cells.
Blood. 115 (6): 1238-46. -
Thorogate, R. et al. (2008) A novel fluorescence-based method in forensic science for the detection of blood in situ.
Forensic Sci Int Genet. 2 (4): 363-71. -
Morgan, R.G. et al. (2013) γ-Catenin is overexpressed in acute myeloid leukemia and promotes the stabilization and nuclear localization of β-catenin.
Leukemia. 27 (2): 336-43. -
Alanazi, B. et al. (2019) Integrated nuclear proteomics and transcriptomics identifies S100A4 as a therapeutic target in acute myeloid leukemia.
Leukemia. Oct 14 [Epub ahead of print].
Further Reading
-
Wang, X. et al. (2004) Histone H1-like protein participates in endothelial cell-specific activation of the von Willebrand factor promoter.
Blood. 104 (6): 1725-32.
- RRID
- AB_2114841
4974-7808
151715
153270
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up