Histone H1 antibody | AE-4
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|Mouse anti Human Histone H1 antibody, clone AE-4 recognizes histone H1.
The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.
In blots of rat brain homogenate a duplet is observed at ~27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3)
- Carrier Free
- Nuclei from myeloid leukamia biopsy cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
- 1 Acetone or PFA fixation is recommended
- Histology Positive Control Tissue
References for Histone H1 antibody
Swindle, C.S. & Engler, J.A. (1998) Association of the human papillomavirus type 11 E1 protein with histone H1.
J Virol. 72 (3): 1994-2001.
Hole, P.S. et al. (2010) Ras-induced reactive oxygen species promote growth factor-independent proliferation in human CD34+ hematopoietic progenitor cells.
Blood. 115 (6): 1238-46.
Thorogate, R. et al. (2008) A novel fluorescence-based method in forensic science for the detection of blood in situ.
Forensic Sci Int Genet. 2 (4): 363-71.
Morgan, R.G. et al. (2013) γ-Catenin is overexpressed in acute myeloid leukemia and promotes the stabilization and nuclear localization of β-catenin.
Leukemia. 27 (2): 336-43.
Alanazi, B. et al. (2019) Integrated nuclear proteomics and transcriptomics identifies S100A4 as a therapeutic target in acute myeloid leukemia.
Leukemia. Oct 14 [Epub ahead of print].
Wang, X. et al. (2004) Histone H1-like protein participates in endothelial cell-specific activation of the von Willebrand factor promoter.
Blood. 104 (6): 1725-32.
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