High Mobility Group Protein B1 antibody | 1E6-E10

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Mouse anti Human High Mobility Group Protein B1:Preservative Free

Product Type
Monoclonal Antibody
Clone
1E6-E10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4045Z IF* P* 0.1 mg
Mouse anti Human High Mobility Group Protein B1 antibody, clone 1E6-E10 recognizes human high mobility group protein B1 (HMGB1). HMGB1 belongs to a family of proteins involved in chromatin organisation and DNA structuring. During inflammation HMGB1 is actively secreted by macrophages and functions as a cytokine.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein with a GST tag corresponding to amino acids 1 - 216 of human HMGB1.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P09429 Related reagents
Entrez Gene
HMGB1 Related reagents
GO Terms
GO:0000793 condensed chromosome
GO:0001773 myeloid dendritic cell activation
GO:0002407 dendritic cell chemotaxis
GO:0002437 inflammatory response to antigenic stimulus
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006265 DNA topological change
GO:0006288 base-excision repair, DNA ligation
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0008301 DNA bending activity
GO:0009986 cell surface
GO:0017055 negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
GO:0031175 neuron projection development
GO:0033151 V(D)J recombination
GO:0042056 chemoattractant activity
GO:0043065 positive regulation of apoptosis
GO:0043280 positive regulation of caspase activity
GO:0043388 positive regulation of DNA binding
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050786 RAGE receptor binding
GO:0070491 repressing transcription factor binding
Regulatory
For research purposes only

Applications of High Mobility Group Protein B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1 5.0 10 ug/ml
Immunohistology - Paraffin 2 1.0 10 ug/ml
  1. 1This product requires fixation of cells for 15 minutes.

    4 % paraformaldehyde solution (NaOH in PBS) pH 7.4 is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F