HIF1 Alpha antibody | Halpha111a

Mouse anti Human HIF1 Alpha

Product Type
Monoclonal Antibody
Clone
Halpha111a
Isotype
IgG2a
Specificity
HIF1 Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA5644GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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loader
MCA5644
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.2 mg loader
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loader
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Mouse anti Human HIF1 alpha antibody, clone Halpha111a recognizes human Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia (Kubis et al. 2005).

HIF1A and HIF1B, also known as ARNT form the highly-conserved heterodimeric HIF-1 transcriptional complex (Carmeliet et al. 1998). During hypoxic conditions, HIF1A accumulates in the nucleus and activates the transcription of many genes encoding proteins involved in the production of oxygen delivery and metabolic adaptation, such as those for vascular endothelial growth factor (VEGFA) . Under normal oxygen conditions, HIF1A is targeted by hypoxia inducible factor prolyl-hydroxylases, followed by rapid protease degradation, which is inhibited during hypoxia (Hirsilä et al. 2003). Overexpression of HIF1A occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Shibaji et al. 2003).

Mouse anti Human HIF1α, clone Halpha111a has been successfully used for the identifcation of HIF1α in human samples by both immunohistochemistry and western blotting.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA5644GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA5644: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant human HIF1 alpha.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/25
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    EDTA pH9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

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Further Reading

  1. Semenza, G.L. (2000) HIF-1 and human disease: one highly involved factor.
    Genes Dev. 14 (16): 1983-91.

Synonyms
HYPOXIA-INDUCIBLE Factor 1-alpha
RRID
AB_10671910
UniProt
Q16665
Entrez Gene
HIF1A
GO Terms
GO:0001666 response to hypoxia
GO:0001837 epithelial to mesenchymal transition
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002248 connective tissue replacement involved in inflammatory response wound healing
GO:0003677 DNA binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0004871 signal transducer activity
GO:0005737 cytoplasm
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0008134 transcription factor binding
GO:0051879 Hsp90 protein binding
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010634 positive regulation of epithelial cell migration
GO:0046886 positive regulation of hormone biosynthetic process
GO:0030528 transcription regulator activity
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0032364 oxygen homeostasis
GO:0032722 positive regulation of chemokine production
GO:0032909 regulation of transforming growth factor-beta2 production
GO:0032963 collagen metabolic process
GO:0035035 histone acetyltransferase binding
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043193 positive regulation of gene-specific transcription
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045766 positive regulation of angiogenesis
GO:0045821 positive regulation of glycolysis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046982 protein heterodimerization activity
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051541 elastin metabolic process
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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