hCG antibody | P1G7F12 (BIO-BCG-005)
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Mouse anti hCG Beta (Free Specific)
- Product Type
- Monoclonal Antibody
- Clone
- P1G7F12 (BIO-BCG-005)
- Isotype
- IgG1
- Specificity
- hCG
- Region
- BETA (FREE SPECIFIC)
Antibody Quality and 
Unrivaled 
| Mouse anti hCG beta (free specific) antibody, clone P1G7F12 (BIO-BCG-005) recognizes the beta subunit of human chorionic gonadotropin (hCG). Minimal cross-reactivity (0.5%) has been observed with the intact hCG molecule and negligible reactivity has been shown with the human luteinizing hormone (LH), follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH). Human chorionic gonadotropin exists in 5 different variants (hCG, sulfated hCG, hyperglycosylated hCG, free beta hCG and hyperglycosylated free beta) that share the same amino acid sequence but have different functions (Cole 2012). hCG is a glycoprotein of 237 amino acids. Regular hCG has a molecular mass of ~36 kDa where oligosaccharides account for 25-30% of the MW (Cole 2010). hCG is composed of a 92-amino acid alpha subunit, common to the LH, FSH and TSH hormones, and of a unique beta subunit, which is recognized by Mouse anti hCG beta antibody, clone P1G7F12. Production of progesterone is not the only role of hCG: it also has numerous other functions, during pregnancy and as a marker for certain cancers (Cole 2012), (Cole 2010) and (Cole 2009). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Human Chorionic Gonadotropin beta subunit from human pregnancy urine
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3X63-Ag 8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Radioimmunoassays |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Further Reading
-
Cole, L.A. (2009) Human chorionic gonadotropin tests.
Expert Rev Mol Diagn. 9:721-47 -
Cole, L.A. (2010) Biological functions of hCG and hCG-related molecules.
Reprod Biol Endocrinol. 8:102-115. -
Cole, L.A. (2012) hCG, the wonder of today's science.
Reprod Biol Endocrinol. 10:24-41
- RRID
- AB_2078963
- UniProt
- P01233
- Entrez Gene
- CGB
View more products with HCG specificity
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