hCG antibody | INN-hCG-22
Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 (MCA329) used for the detection of human choriogonadotrophin expressing cells in urothelium by immunofluorescence.
Image caption:
HCG-beta (beta-2 epitope) immunoreactivity in urothelium. (a) Control bladder from a 36-year-old woman, gynecologic tumor, no bladder carcinoma; (c) BPS/IC bladder of a 53-year-old woman showing extreme upregulation of HCG-beta; bar in (a) applies to (a and b); (c) Quantitative analysis revealed significant differences between female controls () and female BPS/IC () patients (Mann-Whitney test, ).
From: Neuhaus J, Schwalenberg T, Horn LC, Alexander H, Stolzenburg JU.
New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
Adv Urol. 2011;2011:639479.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti hCG (Beta 2 Epitope)
- Product Type
- Monoclonal Antibody
- Clone
- INN-hCG-22
- Isotype
- IgG1
- Specificity
- hCG
- Region
- (BETA 2 EPITOPE)
Antibody Quality and Performance Guarantee
| Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 recognizes the beta subunit of human choriogonadotrophin (hCG), also known as chorionic gonadotrophin. hCGβ is a 165 amino acid ~18 kDa hormone involved in the stimulation of steroid production essential to the maintenance of pregnancy. Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 shows a strong reaction in RIA with intact hCG and hCGβ and some reactivity with human luteinizing hormone (12%) and b-hLH (34%). No reaction with human follicle-stimulating hormone, thyroid-stimulating hormone , a-hCG or a-hLH. Affinity constant = 1.6 x 109m (Ka) . |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- hCG.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse myeloma cell line P3 - NS1/1 - Ag 4.1.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/500 |
| Immunoassay | |
||
| Immunofluorescence | |
||
| Immunohistology - Paraffin | |
||
| Western Blotting | |
Non-reducing conditions |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Source Reference
-
Kofler, R. et al. (1982) Monoclonal antibodies against human chorionic gonadotropin (hCG): I. production, specificity, and intramolecular binding sites.
Am J Reprod Immunol. 2 (4): 212-6.
References for hCG antibody
-
Schwarz, S. et al. (1985) Epitope-selective, monoclonal-antibody-based immunoradiometric assays of predictable specificity for differential measurement of choriogonadotropin and its subunits.
Clin Chem. 31 (8): 1322-8. -
Birken, S. et al. (2003) Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites.
Clin Chem. 49: 144-54. -
Zimmermann, G. et al. (2003) Expression of beta hCG and alpha CG mRNA and hCG hormone in human decidual tissue in patients during tubal pregnancy.
Mol Hum Reprod. 9: 81-9. -
Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
Mol Endocrinol. 19:1803-11. -
Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
Biol Reprod. 80:1053-65. -
Yoshie, M. et al. (2010) Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells.
Hum Reprod. 25 (9): 2229-38. -
Neuhaus, J. et al. (2011) New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
Adv Urol. ;2011:639479. -
Aye, I.L.M.H. et al. (2011) Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors
Placenta. 32: 183-91.
View The Latest Product References
-
Zimmermann, G. et al. (2012) Expression and production of human chorionic gonadotropin (hCG) in the normal secretory endometrium: evidence of CGB7 and/or CGB6 beta hCG subunit gene expression.
Biol Reprod. 86 (3): 87.
- RRID
- AB_2276154
- UniProt
- P01233
- Entrez Gene
- CGB
MCA329
151491
158551
166328
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