hCG antibody | INN-hCG-22

100% Secure


Mouse anti hCG (Beta 2 Epitope)

Product Type
Monoclonal Antibody
Clone
INN-hCG-22
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA329 E IF IY P WB 0.5 mg
Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 recognizes the beta subunit of human choriogonadotrophin (hCG), also known as chorionic gonadotrophin. hCGβ is a 165 amino acid ~18 kDa hormone involved in the stimulation of steroid production essential to the maintenance of pregnancy.

Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 shows a strong reaction in RIA with intact hCG and hCGβ and some reactivity with human luteinizing hormone (12%) and b-hLH (34%). No reaction with human follicle-stimulating hormone, thyroid-stimulating hormone , a-hCG or a-hLH.

Affinity constant = 1.6 x 109m (Ka) .

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
hCG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse myeloma cell line P3 - NS1/1 - Ag 4.1.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01233 Related reagents
Entrez Gene
CGB Related reagents
Regulatory
For research purposes only

Applications of hCG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/500
Immunoassay
Immunofluorescence
Immunohistology - Paraffin
Western Blotting Non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for hCG antibody

  1. Kofler, R. et al. (1982) Monoclonal antibodies against human chorionic gonadotropin (hCG): I. production, specificity, and intramolecular binding sites.
    Am J Reprod Immunol. 2 (4): 212-6.
  2. Schwarz, S. et al. (1985) Epitope-selective, monoclonal-antibody-based immunoradiometric assays of predictable specificity for differential measurement of choriogonadotropin and its subunits.
    Clin Chem. 31 (8): 1322-8.
  3. Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
    Biol Reprod. 80:1053-65.
  4. Yoshie, M. et al. (2010) Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells.
    Hum Reprod. 25 (9): 2229-38.
  5. Zimmermann, G. et al. (2003) Expression of beta hCG and alpha CG mRNA and hCG hormone in human decidual tissue in patients during tubal pregnancy.
    Mol Hum Reprod. 9: 81-9.
  6. Birken, S. et al. (2003) Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites.
    Clin Chem. 49: 144-54.
  7. Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
    Mol Endocrinol. 19:1803-11.
  8. Aye, I.L.M.H. et al. (2011) Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors
    Placenta. 32: 183-91.
  9. Zimmermann, G. et al. (2012) Expression and production of human chorionic gonadotropin (hCG) in the normal secretory endometrium: evidence of CGB7 and/or CGB6 beta hCG subunit gene expression.
    Biol Reprod. 86 (3): 87.
  10. Neuhaus, J. et al. (2011) New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
    Adv Urol. ;2011:639479.