hCG antibody | INN-hCG-22
Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 shows a strong reaction in RIA with intact hCG and hCGβ and some reactivity with human luteinizing hormone (12%) and b-hLH (34%). No reaction with human follicle-stimulating hormone, thyroid-stimulating hormone , a-hCG or a-hLH.
Affinity constant = 1.6 x 109m (Ka) .
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- hCG.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse myeloma cell line P3 - NS1/1 - Ag 4.1.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P01233 Related reagents
- Entrez Gene
- CGB Related reagents
- Regulatory
- For research purposes only
Applications of hCG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100 | 1/500 | |
Immunoassay | |||
Immunofluorescence | |||
Immunohistology - Paraffin | |||
Western Blotting | Non-reducing conditions |
Secondary Antibodies Available
Product Specific References
References for hCG antibody
-
Kofler, R. et al. (1982) Monoclonal antibodies against human chorionic gonadotropin (hCG): I. production, specificity, and intramolecular binding sites.
Am J Reprod Immunol. 2 (4): 212-6. -
Schwarz, S. et al. (1985) Epitope-selective, monoclonal-antibody-based immunoradiometric assays of predictable specificity for differential measurement of choriogonadotropin and its subunits.
Clin Chem. 31 (8): 1322-8. -
Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
Biol Reprod. 80:1053-65. -
Yoshie, M. et al. (2010) Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells.
Hum Reprod. 25 (9): 2229-38. -
Zimmermann, G. et al. (2003) Expression of beta hCG and alpha CG mRNA and hCG hormone in human decidual tissue in patients during tubal pregnancy.
Mol Hum Reprod. 9: 81-9. -
Birken, S. et al. (2003) Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites.
Clin Chem. 49: 144-54. -
Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
Mol Endocrinol. 19:1803-11. -
Aye, I.L.M.H. et al. (2011) Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors
Placenta. 32: 183-91. -
Zimmermann, G. et al. (2012) Expression and production of human chorionic gonadotropin (hCG) in the normal secretory endometrium: evidence of CGB7 and/or CGB6 beta hCG subunit gene expression.
Biol Reprod. 86 (3): 87. -
Neuhaus, J. et al. (2011) New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
Adv Urol. ;2011:639479. -
Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration.
Assay Drug Dev Technol. 14 (5): 298-307.