hCG antibody | INN-hCG-22

Mouse anti hCG (Beta 2 Epitope)

Product Type
Monoclonal Antibody
Clone
INN-hCG-22
Isotype
IgG1
Specificity
hCG
Region
(BETA 2 EPITOPE)

Product Code Applications Pack Size List Price Your Price Qty
MCA329
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IF IY P WB 0.5 mg loader
List Price Your Price
loader
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Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 recognizes the beta subunit of human choriogonadotrophin (hCG), also known as chorionic gonadotrophin. hCGβ is a 165 amino acid ~18 kDa hormone involved in the stimulation of steroid production essential to the maintenance of pregnancy.

Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 shows a strong reaction in RIA with intact hCG and hCGβ and some reactivity with human luteinizing hormone (12%) and b-hLH (34%). No reaction with human follicle-stimulating hormone, thyroid-stimulating hormone , a-hCG or a-hLH.

Affinity constant = 1.6 x 109m (Ka) .

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
hCG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse myeloma cell line P3 - NS1/1 - Ag 4.1.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/500
Immunoassay
Immunofluorescence
Immunohistology - Paraffin
Western Blotting Non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for hCG antibody

  1. Kofler, R. et al. (1982) Monoclonal antibodies against human chorionic gonadotropin (hCG): I. production, specificity, and intramolecular binding sites.
    Am J Reprod Immunol. 2 (4): 212-6.
  2. Schwarz, S. et al. (1985) Epitope-selective, monoclonal-antibody-based immunoradiometric assays of predictable specificity for differential measurement of choriogonadotropin and its subunits.
    Clin Chem. 31 (8): 1322-8.
  3. Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
    Biol Reprod. 80:1053-65.
  4. Yoshie, M. et al. (2010) Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells.
    Hum Reprod. 25 (9): 2229-38.
  5. Zimmermann, G. et al. (2003) Expression of beta hCG and alpha CG mRNA and hCG hormone in human decidual tissue in patients during tubal pregnancy.
    Mol Hum Reprod. 9: 81-9.
  6. Birken, S. et al. (2003) Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites.
    Clin Chem. 49: 144-54.
  7. Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
    Mol Endocrinol. 19:1803-11.
  8. Aye, I.L.M.H. et al. (2011) Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors
    Placenta. 32: 183-91.
  9. View The Latest Product References
  10. Zimmermann, G. et al. (2012) Expression and production of human chorionic gonadotropin (hCG) in the normal secretory endometrium: evidence of CGB7 and/or CGB6 beta hCG subunit gene expression.
    Biol Reprod. 86 (3): 87.
  11. Neuhaus, J. et al. (2011) New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
    Adv Urol. ;2011:639479.
  12. Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration.
    Assay Drug Dev Technol. 14 (5): 298-307.

Immunofluorescence

Western Blotting

RRID
AB_2276154
UniProt
P01233
Entrez Gene
CGB
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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