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hCG antibody | INN-hCG-22

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Anti hCG (Beta 2 Epitope) Antibody, clone INN-hCG-22 gallery image 1
Published customer image:
Mouse anti Human chorionic gonadotrophin antibody, clone INN-hCG-22 (MCA329) used for the detection of human choriogonadotrophin expressing cells in urothelium by immunofluorescence.
Image caption:
HCG-beta (beta-2 epitope) immunoreactivity in urothelium. (a) Control bladder from a 36-year-old woman, gynecologic tumor, no bladder carcinoma; (c) BPS/IC bladder of a 53-year-old woman showing extreme upregulation of HCG-beta; bar in (a) applies to (a and b); (c) Quantitative analysis revealed significant differences between female controls () and female BPS/IC () patients (Mann-Whitney test, ).

From: Neuhaus J, Schwalenberg T, Horn LC, Alexander H, Stolzenburg JU.
New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
Adv Urol. 2011;2011:639479.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti hCG (Beta 2 Epitope)

Product Type
Monoclonal Antibody
Clone
INN-hCG-22
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA329
Datasheet
E IF IY P WB
SDS
0.5 mg loader

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01233
Entrez Gene
CGB
Regulatory
For research purposes only

Applications of hCG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/500
Immunoassay
Immunofluorescence
Immunohistology - Paraffin
Western Blotting Non-reducing conditions
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for hCG antibody

  1. Kofler, R. et al. (1982) Monoclonal antibodies against human chorionic gonadotropin (hCG): I. production, specificity, and intramolecular binding sites.
    Am J Reprod Immunol. 2 (4): 212-6.
  2. Schwarz, S. et al. (1985) Epitope-selective, monoclonal-antibody-based immunoradiometric assays of predictable specificity for differential measurement of choriogonadotropin and its subunits.
    Clin Chem. 31 (8): 1322-8.
  3. Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
    Biol Reprod. 80:1053-65.
  4. Yoshie, M. et al. (2010) Possible role of the exchange protein directly activated by cyclic AMP (Epac) in the cyclic AMP-dependent functional differentiation and syncytialization of human placental BeWo cells.
    Hum Reprod. 25 (9): 2229-38.
  5. Zimmermann, G. et al. (2003) Expression of beta hCG and alpha CG mRNA and hCG hormone in human decidual tissue in patients during tubal pregnancy.
    Mol Hum Reprod. 9: 81-9.
  6. Birken, S. et al. (2003) Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites.
    Clin Chem. 49: 144-54.
  7. Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
    Mol Endocrinol. 19:1803-11.
  8. Aye, I.L.M.H. et al. (2011) Oxysterols inhibit differentiation and fusion of term primary trophoblasts by activating liver X receptors
    Placenta. 32: 183-91.
  9. Zimmermann, G. et al. (2012) Expression and production of human chorionic gonadotropin (hCG) in the normal secretory endometrium: evidence of CGB7 and/or CGB6 beta hCG subunit gene expression.
    Biol Reprod. 86 (3): 87.
  10. Neuhaus, J. et al. (2011) New aspects in the differential diagnosis and therapy of bladder pain syndrome/interstitial cystitis.
    Adv Urol. ;2011:639479.
  11. Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration.
    Assay Drug Dev Technol. 14 (5): 298-307.

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