Growth Hormone antibody | 5B4
Mouse anti hGH, Clone 5B4, recognises human growth hormone, also known as somatotropin, a polypeptide synthesised by acidophilic or somatotropic cells of the anterior pituitary gland. It exists as several isoforms, including a ~20 kDa and ~22 kDa variant. Growth hormone plays an important role in growth and cell production.
Cross reactivity profile:
Cross reactivity profile:
|Human growth hormone 22 kDa||100%|
|Human placental growth hormone||100%|
|Human growth hormone 20 kDa||50%|
|Methionyl human growth hormone||0.5%|
|Growth hormone receptor||0.3%|
|Human placental lactogen||0.002%|
|Human thyroid stimulating hormone||0%|
|Human chorionic gonadotropin||0%|
|Human luteinizing hormone||0%|
|Human follicle stimulating hormone||0%|
|Horse growth hormone||0.007%|
|Bovine growth hormone||0%|
|Pig growth hormone||0%|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Bovine Pig Horse Weak
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P01241 Related reagents
- Entrez Gene
- GH1 Related reagents
- GO Terms
- GO:0005131 growth hormone receptor binding
- GO:0005148 prolactin receptor binding
- GO:0005179 hormone activity
- GO:0046872 metal ion binding
- GO:0005615 extracellular space
- GO:0007259 JAK-STAT cascade
- GO:0008083 growth factor activity
- GO:0010535 positive regulation of activation of JAK2 kinase activity
- GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
- GO:0015758 glucose transport
- GO:0032355 response to estradiol stimulus
- GO:0040018 positive regulation of multicellular organism growth
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043406 positive regulation of MAP kinase activity
- GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
- GO:0060396 growth hormone receptor signaling pathway
- For research purposes only
Applications of Growth Hormone antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Growth Hormone antibody
Gomez, F. et al. (1984) A highly sensitive radioimmunoassay for human growth hormone using a monoclonal antibody.
J Immunoassay. 5:145-57.
Hennen, G. et al. (1985) A human placental GH: increasing levels during second half of pregnancy with pituitary GH suppression as revealed by monoclonal antibody radioimmunoassays.
Int J Fertil. 30: 27-33.
Frankenne, F. et al. (1987) [Discovery of a placental variant of human growth hormone: biochemistry, physiology and implication in the secretion of hypophyseal forms].
Reprod Nutr Dev. 27: 523-4.
Frankenne, F. et al. (1988) The physiology of growth hormones (GHs) in pregnant women and partial characterization of the placental GH variant.
J Clin Endocrinol Metab. 66: 1171-80.
Igout, A. et al. (1989) Expression and secretion of the human placental growth hormone in Escherichia coli.
Nucleic Acids Res. 17: 3998.
Eriksson, L. et al. (1989) Growth hormone 24-h serum profiles during pregnancy--lack of pulsatility for the secretion of the placental variant.
Br J Obstet Gynaecol. 96: 949-53.
Jara, C.S. et al. (1989) Immunocytochemical localization of the human growth hormone variant in the human placenta.
J Clin Endocrinol Metab. 69: 1069-72.
Mirlesse, V. et al. (1993) Placental growth hormone levels in normal pregnancy and in pregnancies with intrauterine growth retardation.
Pediatr Res. 34: 439-42.
Igout, A. et al. (1993) Purification and biochemical characterization of recombinant human placental growth hormone produced in Escherichia coli.
Biochem J. 295: 719-24.