Growth Hormone antibody | 5B4

100% Secure


Mouse anti Human Growth Hormone

Product Type
Monoclonal Antibody
Clone
5B4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5830G E 1 mg
Mouse anti hGH, Clone 5B4, recognises human growth hormone, also known as somatotropin, a polypeptide synthesised by acidophilic or somatotropic cells of the anterior pituitary gland. It exists as several isoforms, including a ~20 kDa and ~22 kDa variant. Growth hormone plays an important role in growth and cell production.

Cross reactivity profile:

CompoundReactivity
Human growth hormone 22 kDa100%
Human placental growth hormone100%
Human growth hormone 20 kDa 50%
Methionyl human growth hormone0.5%
Growth hormone receptor0.3%
Human placental lactogen0.002%
Human thyroid stimulating hormone 0%
Human chorionic gonadotropin0%
Human luteinizing hormone0%
Human follicle stimulating hormone 0%
Human prolactin0%
Horse growth hormone0.007%
Bovine growth hormone0%
Pig growth hormone0%


Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Pig
Horse Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01241 Related reagents
Entrez Gene
GH1 Related reagents
GO Terms
GO:0005131 growth hormone receptor binding
GO:0005148 prolactin receptor binding
GO:0005179 hormone activity
GO:0046872 metal ion binding
GO:0005615 extracellular space
GO:0007259 JAK-STAT cascade
GO:0008083 growth factor activity
GO:0010535 positive regulation of activation of JAK2 kinase activity
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0015758 glucose transport
GO:0032355 response to estradiol stimulus
GO:0040018 positive regulation of multicellular organism growth
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043406 positive regulation of MAP kinase activity
GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
GO:0060396 growth hormone receptor signaling pathway
Regulatory
For research purposes only

Applications of Growth Hormone antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/50000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Growth Hormone antibody

  1. Gomez, F. et al. (1984) A highly sensitive radioimmunoassay for human growth hormone using a monoclonal antibody.
    J Immunoassay. 5:145-57.
  2. Hennen, G. et al. (1985) A human placental GH: increasing levels during second half of pregnancy with pituitary GH suppression as revealed by monoclonal antibody radioimmunoassays.
    Int J Fertil. 30: 27-33.
  3. Frankenne, F. et al. (1987) [Discovery of a placental variant of human growth hormone: biochemistry, physiology and implication in the secretion of hypophyseal forms].
    Reprod Nutr Dev. 27: 523-4.
  4. Frankenne, F. et al. (1988) The physiology of growth hormones (GHs) in pregnant women and partial characterization of the placental GH variant.
    J Clin Endocrinol Metab. 66: 1171-80.
  5. Igout, A. et al. (1989) Expression and secretion of the human placental growth hormone in Escherichia coli.
    Nucleic Acids Res. 17: 3998.
  6. Eriksson, L. et al. (1989) Growth hormone 24-h serum profiles during pregnancy--lack of pulsatility for the secretion of the placental variant.
    Br J Obstet Gynaecol. 96: 949-53.
  7. Jara, C.S. et al. (1989) Immunocytochemical localization of the human growth hormone variant in the human placenta.
    J Clin Endocrinol Metab. 69: 1069-72.
  8. Mirlesse, V. et al. (1993) Placental growth hormone levels in normal pregnancy and in pregnancies with intrauterine growth retardation.
    Pediatr Res. 34: 439-42.
  9. Igout, A. et al. (1993) Purification and biochemical characterization of recombinant human placental growth hormone produced in Escherichia coli.
    Biochem J. 295: 719-24.