Growth Hormone antibody | 5B4
Mouse anti Human Growth Hormone
- Product Type
- Monoclonal Antibody
- Clone
- 5B4
- Isotype
- IgG2a
- Specificity
- Growth Hormone
Antibody Quality and Performance Guarantee
| Mouse anti hGH, Clone 5B4, recognises human growth hormone, also known as somatotropin, a polypeptide synthesised by acidophilic or somatotropic cells of the anterior pituitary gland. It exists as several isoforms, including a ~20 kDa and ~22 kDa variant. Growth hormone plays an important role in growth and cell production. Cross reactivity profile:
|
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Pig Horse Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | 1/50000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Growth Hormone antibody
-
Gomez, F. et al. (1984) A highly sensitive radioimmunoassay for human growth hormone using a monoclonal antibody.
J Immunoassay. 5:145-57. -
Hennen, G. et al. (1985) A human placental GH: increasing levels during second half of pregnancy with pituitary GH suppression as revealed by monoclonal antibody radioimmunoassays.
Int J Fertil. 30: 27-33. -
Frankenne, F. et al. (1987) [Discovery of a placental variant of human growth hormone: biochemistry, physiology and implication in the secretion of hypophyseal forms].
Reprod Nutr Dev. 27: 523-4. -
Frankenne, F. et al. (1988) The physiology of growth hormones (GHs) in pregnant women and partial characterization of the placental GH variant.
J Clin Endocrinol Metab. 66: 1171-80. -
Igout, A. et al. (1989) Expression and secretion of the human placental growth hormone in Escherichia coli.
Nucleic Acids Res. 17: 3998. -
Eriksson, L. et al. (1989) Growth hormone 24-h serum profiles during pregnancy--lack of pulsatility for the secretion of the placental variant.
Br J Obstet Gynaecol. 96: 949-53. -
Jara, C.S. et al. (1989) Immunocytochemical localization of the human growth hormone variant in the human placenta.
J Clin Endocrinol Metab. 69: 1069-72. -
Mirlesse, V. et al. (1993) Placental growth hormone levels in normal pregnancy and in pregnancies with intrauterine growth retardation.
Pediatr Res. 34: 439-42.
View The Latest Product References
-
Igout, A. et al. (1993) Purification and biochemical characterization of recombinant human placental growth hormone produced in Escherichia coli.
Biochem J. 295: 719-24.
- Synonyms
- Somatotropin
- UniProt
- P01241
- Entrez Gene
- GH1
- GO Terms
- GO:0005131 growth hormone receptor binding
- GO:0005148 prolactin receptor binding
- GO:0005179 hormone activity
- GO:0046872 metal ion binding
- GO:0005615 extracellular space
- GO:0007259 JAK-STAT cascade
- GO:0008083 growth factor activity
- GO:0010535 positive regulation of activation of JAK2 kinase activity
- GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
- View More GO Terms
- GO:0015758 glucose transport
- GO:0032355 response to estradiol stimulus
- GO:0040018 positive regulation of multicellular organism growth
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043406 positive regulation of MAP kinase activity
- GO:0043568 positive regulation of insulin-like growth factor receptor signaling pathway
- GO:0060396 growth hormone receptor signaling pathway
MCA5830G
0112R
159442
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