Mouse anti hGH, Clone 5B4,
recognises human growth hormone, also known as somatotropin, a polypeptide synthesised by acidophilic or somatotropic cells of the anterior pituitary gland. It exists as several isoforms, including a ~20 kDa and ~22 kDa variant. Growth hormone plays an important role in growth and cell production.Cross reactivity profile:
|Human growth hormone 22 kDa||100%|
|Human placental growth hormone||100%|
|Human growth hormone 20 kDa ||50%|
|Methionyl human growth hormone||0.5%|
|Growth hormone receptor||0.3%|
|Human placental lactogen||0.002%|
|Human thyroid stimulating hormone ||0%|
|Human chorionic gonadotropin||0%|
|Human luteinizing hormone||0%|
|Human follicle stimulating hormone ||0%|
|Horse growth hormone||0.007%|
|Bovine growth hormone||0%|
|Pig growth hormone||0%|
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
growth hormone receptor binding
prolactin receptor binding
metal ion binding
growth factor activity
positive regulation of activation of JAK2 kinase activity
positive regulation of phosphatidylinositol 3-kinase cascade
response to estradiol stimulus
positive regulation of multicellular organism growth
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of MAP kinase activity
positive regulation of insulin-like growth factor receptor signaling pathway
growth hormone receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Growth Hormone antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Growth Hormone antibody
Gomez, F. et al. (1984) A highly sensitive radioimmunoassay for human growth hormone using a monoclonal antibody.
J Immunoassay. 5:145-57.
Hennen, G. et al. (1985) A human placental GH: increasing levels during second half of pregnancy with pituitary GH suppression as revealed by monoclonal antibody radioimmunoassays.
Int J Fertil. 30: 27-33.
Frankenne, F. et al. (1987) [Discovery of a placental variant of human growth hormone: biochemistry, physiology and implication in the secretion of hypophyseal forms].
Reprod Nutr Dev. 27: 523-4.
Frankenne, F. et al. (1988) The physiology of growth hormones (GHs) in pregnant women and partial characterization of the placental GH variant.
J Clin Endocrinol Metab. 66: 1171-80.
Igout, A. et al. (1989) Expression and secretion of the human placental growth hormone in Escherichia coli.
Nucleic Acids Res. 17: 3998.
Eriksson, L. et al. (1989) Growth hormone 24-h serum profiles during pregnancy--lack of pulsatility for the secretion of the placental variant.
Br J Obstet Gynaecol. 96: 949-53.
Jara, C.S. et al. (1989) Immunocytochemical localization of the human growth hormone variant in the human placenta.
J Clin Endocrinol Metab. 69: 1069-72.
Mirlesse, V. et al. (1993) Placental growth hormone levels in normal pregnancy and in pregnancies with intrauterine growth retardation.
Pediatr Res. 34: 439-42.
Igout, A. et al. (1993) Purification and biochemical characterization of recombinant human placental growth hormone produced in Escherichia coli.
Biochem J. 295: 719-24.