Glutamate Decarboxylase 2 antibody | N-GAD65
Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of γ-aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system (Fenalti et al. 2007) and is expressed in the brain and pancreatic beta cells.
Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmann et al. 1997), Stiff Man Syndrome (Raju et al. 2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersen et al. 1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shi et al. 1994).
Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al. 2001).
Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmann et al. 1997), Stiff Man Syndrome (Raju et al. 2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersen et al. 1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shi et al. 1994).
Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al. 2001).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q05329 Related reagents
- Entrez Gene
- GAD2 Related reagents
- GO Terms
- GO:0000139 Golgi membrane
- GO:0005515 protein binding
- GO:0042136 neurotransmitter biosynthetic process
- GO:0004351 glutamate decarboxylase activity
- GO:0005829 cytosol
- GO:0006540 glutamate decarboxylation to succinate
- GO:0007269 neurotransmitter secretion
- GO:0030054 cell junction
- GO:0030170 pyridoxal phosphate binding
- GO:0042734 presynaptic membrane
- GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane
- Regulatory
- For research purposes only
Applications of Glutamate Decarboxylase 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Western Blotting | 1/100 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human brain
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for Glutamate Decarboxylase 2 antibody
-
Hampe, C.S. et al. (2001) A novel monoclonal antibody specific for the N-terminal end of GAD65.
J Neuroimmunol. 113 (1): 63-71.
