Glutamate Decarboxylase 2 antibody | N-GAD65

Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal)

Product Type
Monoclonal Antibody
Clone
N-GAD65
Isotype
IgG1
Specificity
Glutamate Decarboxylase 2
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
MCA5648GA
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SDS Safety Datasheet SDS
C IP P R WB 0.1 mg loader
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loader
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Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of γ-aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system (Fenalti et al. 2007) and is expressed in the brain and pancreatic beta cells.

Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmann et al. 1997), Stiff Man Syndrome (Raju et al. 2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersen et al. 1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shi et al. 1994).

Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al. 2001).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Radioimmunoassays
Western Blotting 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain

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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for Glutamate Decarboxylase 2 antibody

  1. Hampe, C.S. et al. (2001) A novel monoclonal antibody specific for the N-terminal end of GAD65.
    J Neuroimmunol. 113 (1): 63-71.

Synonyms
GAD65/GAD2
RRID
AB_10922594
UniProt
Q05329
Entrez Gene
GAD2
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0042136 neurotransmitter biosynthetic process
GO:0004351 glutamate decarboxylase activity
GO:0005829 cytosol
GO:0006540 glutamate decarboxylation to succinate
GO:0007269 neurotransmitter secretion
GO:0030054 cell junction
GO:0030170 pyridoxal phosphate binding
GO:0042734 presynaptic membrane
GO:0061202 clathrin sculpted gamma-aminobutyric acid transport vesicle membrane