Glucocorticoid Receptor antibody | 8E9

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Mouse anti Human Glucocorticoid Receptor

Product Type
Monoclonal Antibody
Clone
8E9
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1390 E WB 0.2 mg
Mouse anti Human glucocorticoid receptor antibody, clone 8E9 recognizes the human glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1. Human GR is a 777 amino acid ~97kDa (Moraes et al. 2005) member of the NR3 subfamily of nuclear hormone receptors, bearing a single nuclear receptor DNA-binding domain. Multiple isoforms of the human glucocorticoid receptor are generated by either alternative splicing or alternative initiation (UniProt:: P04150).

In the absence of bound ligand GRs are located in the cytoplasm and are translocated to the nucleus or mitochondrion following ligation (Rossini et al. 1984). GRs are associated with heat shock proteins in the cytoplasm when ligated to steroid hormone, being disrupted on translocation of the steroid:receptor complex to the nucleus (Tse et al. 2011). Mouse anti Human glucocorticoid receptor antibody, clone 8E9 was raised against a conserved region of the glucocorticoid receptor and recognizes human GR, binding to an epitope between amino acids 167-176 and is therefore expected to bind all described isoforms of the human glucocorticoid receptor.

Mouse anti Human glucocorticoid receptor antibody, clone 8E9 has been used successfully for the identification of human glucocorticoid receptor using flow cytometry (Berki et al. 1998), western blotting (Moraes et al. 2005) and immunoprecipitation where it has also been shown to bind to the murine GR (Paul-Clark et al. 2003, Bartis et al. 2007).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.1%Bovine Serum Albumin
Immunogen
26 amino acid peptide corresponding to residues 150-176 of human GCR linked to human thyroglobulin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04150 Related reagents
Entrez Gene
NR3C1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0004883 glucocorticoid receptor activity
GO:0005496 steroid binding
GO:0005654 nucleoplasm
GO:0005759 mitochondrial matrix
GO:0006366 transcription from RNA polymerase II promoter
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0043565 sequence-specific DNA binding
GO:0051789 response to protein stimulus
GO:0071109 superior temporal gyrus development
Regulatory
For research purposes only

Applications of Glucocorticoid Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000
Immunohistology - Frozen
Immunohistology - Paraffin
Immunohistology - Resin
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Glucocorticoid Receptor antibody

  1. Berki, T. et al. (1998) Production and flow cytometric application of a monoclonal anti-glucocorticoid receptor antibody.
    J Immunol Methods. 214 (1-2): 19-27.
  2. Moraes, L.A. et al. (2005) Ligand-specific glucocorticoid receptor activation in human platelets.
    Blood.106: 4167-75.
  3. Paul-Clark, M.J. et al. (2003) Glucocorticoid receptor nitration leads to enhanced anti-inflammatory effects of novel steroid ligands.
    J Immunol. 171: 3245-52.
  4. Bartis, D. et al. (2007) Intermolecular relations between the glucocorticoid receptor, ZAP-70 kinase, and Hsp-90.
    Biochem Biophys Res Commun. 354: 253-8.
  5. Ouyang, J. et al. (2012) Nuclear HSP90 regulates the glucocorticoid responsiveness of PBMCs in patients with idiopathic nephrotic syndrome.
    Int Immunopharmacol. 14 (3): 334-40.
  6. Bourcier, T. et al. (1999) In vitro effects of dexamethasone on human corneal keratocytes.
    Invest Ophthalmol Vis Sci. 40 (6): 1061-70.
  7. Bourcier T et al. (2000) Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone.
    Invest Ophthalmol Vis Sci. 41 (13): 4133-41.
  8. Paul-Clark, M.J. et al. (2003) Glucocorticoid receptor nitration leads to enhanced anti-inflammatory effects of novel steroid ligands.
    J Immunol. 171 (6): 3245-52.