GGT1 antibody | 1F9
Mouse anti Human GGT1 antibody, clone 1F9 recognizes human Gamma-glutamyltranspeptidase 1, also known as GGT1, Leukotriene-C4 hydrolase, CD224 or Glutathione hydrolase 1. GGT1 is a 569 amino acid ~65-70 kDa single pass, type II transmembrane glycoprotein. GGT1 forms part of the cell antioxidant defense mechanism.
Mutations in the GGT1 gene can lead to the development of Glutathionuria (GLUTH) a disease also known as Gamma-glutamyltranspeptidase deficiency.
Mutations in the GGT1 gene can lead to the development of Glutathionuria (GLUTH) a disease also known as Gamma-glutamyltranspeptidase deficiency.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 381-471 of human GGT1
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P19440
- Entrez Gene
- GGT1
- GO Terms
- GO:0005515 protein binding
- GO:0003840 gamma-glutamyltransferase activity
- GO:0016021 integral to membrane
- GO:0006750 glutathione biosynthetic process
- GO:0008415 acyltransferase activity
- Regulatory
- For research purposes only
Applications of GGT1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 0.1 | 10 ug/ml | |
| Western Blotting | 0.1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for GGT1 antibody
-
Blackmore, L. et al. (2013) Polymorphisms in ABCB11 and ATP8B1 Associated with Development of Severe Intrahepatic Cholestasis in Hodgkin's Lymphoma.
J Clin Exp Hepatol. 3 (2): 159-61.
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