GGT1 antibody | 1F9

Mouse anti Human GGT1:Preservative Free

Product Type
Monoclonal Antibody
Clone
1F9
Isotype
IgG2a
Specificity
GGT1

Product Code Applications Pack Size List Price Your Price Qty
MCA3141Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human GGT1 antibody, clone 1F9 recognizes human Gamma-glutamyltranspeptidase 1, also known as GGT1, Leukotriene-C4 hydrolase, CD224 or Glutathione hydrolase 1. GGT1 is a 569 amino acid ~65-70 kDa single pass, type II transmembrane glycoprotein. GGT1 forms part of the cell antioxidant defense mechanism.

Mutations in the GGT1 gene can lead to the development of Glutathionuria (GLUTH) a disease also known as Gamma-glutamyltranspeptidase deficiency.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 381-471 of human GGT1
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
Western Blotting 0.1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

References for GGT1 antibody

  1. Blackmore, L. et al. (2013) Polymorphisms in ABCB11 and ATP8B1 Associated with Development of Severe Intrahepatic Cholestasis in Hodgkin's Lymphoma.
    J Clin Exp Hepatol. 3 (2): 159-61.

Synonyms
CD224
RRID
AB_2279022
UniProt
P19440
Entrez Gene
GGT1
GO Terms
GO:0005515 protein binding
GO:0003840 gamma-glutamyltransferase activity
GO:0016021 integral to membrane
GO:0006750 glutathione biosynthetic process
GO:0008415 acyltransferase activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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