Fibroblasts/Epithelial Cells antibody | D7-FIB


Mouse anti Human Fibroblasts/Epithelial Cells
Mouse anti Human Fibroblasts/Epithelial Cells:RPE
- Product Type
- Monoclonal Antibody
- Clone
- D7-FIB
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1399GT | C* F IP | 25 µg |
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MCA1399G | C* F IP | 0.2 mg |
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MCA1399PE | F | 100 Tests |
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MCA1399PET | F | 25 Tests |
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In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody.
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1.0ml distilled water.Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Human foreskin fibroblasts.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Fibroblasts/Epithelial Cells antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/200 | |
Immunohistology - Frozen 1 | 1/100 | ||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Flow Cytometry | Neat | 1/10 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for Fibroblasts/Epithelial Cells antibody
-
Fearns C & Dowdle EB (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro.
Int J Cancer. 50 (4): 621-7. -
Kelynack, K.J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process.
Nephrol Dial Transplant. 15 (11): 1766-72. -
van Osch, G.J. et al. (2001) Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage.
Biochem Biophys Res Commun. 280 (3): 806-12. -
Behl, B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells.
Biomaterials. 34 (14): 3547-58. -
Morito, T. et al. (2008) Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans.
Rheumatology (Oxford). 47 (8): 1137-43. -
Pountos, I. et al. (2011) NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man.
J Cell Mol Med. 15: 525-34. -
Telfer, J.F. and Brock, J.H. (2002) Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium.
Ann Rheum Dis. 61: 741-4. -
English, A. et al. (2007) A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis.
Rheumatology (Oxford). 46: 1676-83. -
Jones, E.A. et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow.
Cytometry B Clin Cytom. 70: 391-9. -
Nimura, A. et al. (2008) Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum.
Arthritis Rheum. 58: 501-10. -
Miranda-Carús, M.E. et al. (2004) Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active.
Arthritis Rheum. 50: 2786-93. -
Petrow, P.K. et al. (2002) Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis.
Ann Rheum Dis. 61: 391-7. -
Sekiya, I. et al. (2012) Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.
J Orthop Res. 30: 943-9. -
Kanayama, M. et al. (2009) Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis.
J Immunol. 182: 8015-25. -
Pap, T. et al. (2003) Osteoclast-independent bone resorption by fibroblast-like cells
Arthritis Res Ther. 5: R163-73. -
Shi, Y. et al. (2012) Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration.
Tissue Eng Part A. 18: 1313-21. -
Scutt, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments.
J Orthop Surg Res. 3: 32. -
Jones. E.A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.
Arthritis. Rheum. 46: 3349-60. -
Schminke, B. et al. (2015) The pathology of bone tissue during peri-implantitis.
J Dent Res. 94 (2): 354-61. -
De Bari, C. et al. (2006) Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis.
Arthritis Rheum. 54 (4): 1209-21. -
Iyyanki, T. et al. (2015) Harvesting technique affects adipose-derived stem cell yield.
Aesthet Surg J. 35 (4): 467-76. -
Asano, T. et al. (2014) α9β1 integrin acts as a critical intrinsic regulator of human rheumatoid arthritis.
Rheumatology (Oxford). 53 (3): 415-24. -
Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.
J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505. -
De Bari, C. et al. (2008) A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells.
Arthritis Rheum. 58 (1): 240-50. -
Stolzing, A. et al. (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.
Mech Ageing Dev. 129 (3): 163-73. -
Martinez, C. et al. (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs.
Blood. 109 (10): 4245-8. -
Jayne, D.G. et al. (1999) A three-dimensional in-vitro model for the study of peritoneal tumour metastasis.
Clin Exp Metastasis. 17 (6): 515-23. -
Lu, G. et al. (2013) Transplantation-potential-related biological properties of decidua basalis mesenchymal stem cells from maternal human term placenta.
Cell Tissue Res. 352 (2): 301-12. -
Pandya, J.M. et al. (2016) CD4+ and CD8+ CD28(null) T Cells Are Cytotoxic to Autologous Muscle Cells in Patients With Polymyositis.
Arthritis Rheumatol. 68 (8): 2016-26. -
Manoussaka, M.S. et al. (2005) Flow cytometric characterisation of cells of differing densities isolated from human term placentae and enrichment of villous trophoblast cells.
Placenta. 26 (4): 308-18. -
Todisco, E. et al. (2002) CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
Leukemia. 16 (10): 2046-54. -
Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
Cell Transplant. 25 (3): 463-79. -
Broeren, M.G.A. (2019) A three-dimensional model to study human synovial pathology
ALTEX. 36 (1): 18-28.
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