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Fibroblasts/Epithelial Cells antibody | D7-FIB

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Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB thumbnail image 1 Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB thumbnail image 2
Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB gallery image 1
Flow cytometry staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MCA1399G).
Anti Human Fibroblasts/Epithelial Cells Antibody, clone D7-FIB gallery image 2
Published customer image:
Mouse anti Human fibroblasts/ epithelial cells antibody, clone D7-FIB (MCA1399G) used to demonstrate fibroblasts in micromasses by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Composition of micromasses constructed from primary RA FLS and CD14+ PBMCs CD68 staining of macrophages in the micromass. Black arrows indicate CD68+ cells and grey arrows indicate CD68-negative cells. 11-Fibrau staining of FLS in the micromass. Black arrows indicate 11-Fibrau+ cells and grey arrows indicate 11-Fibrau-negative cells.

From: Broeren MGA, Waterborg CEJ, Wiegertjes R, Thurlings RM, Koenders MI, Van Lent PLEM, Van der Kraan PM, Van de Loo FAJ.
A three-dimensional model to study human synovial pathology.
ALTEX. 2019;36(1):18-28.
doi: 10.14573/altex.1804161.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Fibroblasts/Epithelial Cells

Mouse anti Human Fibroblasts/Epithelial Cells:RPE

Product Type
Monoclonal Antibody
Clone
D7-FIB
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1399GT
Datasheet
C* F IP
SDS
25 µg loader

MCA1399G
Datasheet
C* F IP
SDS
0.2 mg loader

MCA1399PE
Datasheet
F
SDS
100 Tests loader

MCA1399PET
Datasheet
F
SDS
25 Tests loader

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.

In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody.

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).

Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1.0ml distilled water.
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Human foreskin fibroblasts.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Store at +4oC. DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Fibroblasts/Epithelial Cells antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1 1/100
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry Neat 1/10
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for Fibroblasts/Epithelial Cells antibody

  1. Fearns C & Dowdle EB (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro.
    Int J Cancer. 50 (4): 621-7.
  2. Kelynack, K.J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process.
    Nephrol Dial Transplant. 15 (11): 1766-72.
  3. van Osch, G.J. et al. (2001) Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage.
    Biochem Biophys Res Commun. 280 (3): 806-12.
  4. Behl, B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells.
    Biomaterials. 34 (14): 3547-58.
  5. Morito, T. et al. (2008) Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans.
    Rheumatology (Oxford). 47 (8): 1137-43.
  6. Pountos, I. et al. (2011) NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man.
    J Cell Mol Med. 15: 525-34.
  7. Telfer, J.F. and Brock, J.H. (2002) Expression of ferritin, transferrin receptor, and non-specific resistance associated macrophage proteins 1 and 2 (Nramp1 and Nramp2) in the human rheumatoid synovium.
    Ann Rheum Dis. 61: 741-4.
  8. English, A. et al. (2007) A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis.
    Rheumatology (Oxford). 46: 1676-83.
  9. Jones, E.A. et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow.
    Cytometry B Clin Cytom. 70: 391-9.
  10. Nimura, A. et al. (2008) Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum.
    Arthritis Rheum. 58: 501-10.
  11. Miranda-Carús, M.E. et al. (2004) Rheumatoid arthritis synovial fluid fibroblasts express TRAIL-R2 (DR5) that is functionally active.
    Arthritis Rheum. 50: 2786-93.
  12. Petrow, P.K. et al. (2002) Characterisation of the cell type-specificity of collagenase 3 mRNA expression in comparison with membrane type 1 matrix metalloproteinase and gelatinase A in the synovial membrane in rheumatoid arthritis.
    Ann Rheum Dis. 61: 391-7.
  13. Sekiya, I. et al. (2012) Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis.
    J Orthop Res. 30: 943-9.
  14. Kanayama, M. et al. (2009) Alpha9 integrin and its ligands constitute critical joint microenvironments for development of autoimmune arthritis.
    J Immunol. 182: 8015-25.
  15. Pap, T. et al. (2003) Osteoclast-independent bone resorption by fibroblast-like cells
    Arthritis Res Ther. 5: R163-73.
  16. Shi, Y. et al. (2012) Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration.
    Tissue Eng Part A. 18: 1313-21.
  17. Scutt, N. et al. (2008) Tissue specific characteristics of cells isolated from human and rat tendons and ligaments.
    J Orthop Surg Res. 3: 32.
  18. Jones. E.A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells.
    Arthritis. Rheum. 46: 3349-60.
  19. Schminke, B. et al. (2015) The pathology of bone tissue during peri-implantitis.
    J Dent Res. 94 (2): 354-61.
  20. De Bari, C. et al. (2006) Mesenchymal multipotency of adult human periosteal cells demonstrated by single-cell lineage analysis.
    Arthritis Rheum. 54 (4): 1209-21.
  21. Iyyanki, T. et al. (2015) Harvesting technique affects adipose-derived stem cell yield.
    Aesthet Surg J. 35 (4): 467-76.
  22. Asano, T. et al. (2014) α9β1 integrin acts as a critical intrinsic regulator of human rheumatoid arthritis.
    Rheumatology (Oxford). 53 (3): 415-24.
  23. Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.
    J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505.
  24. De Bari, C. et al. (2008) A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells.
    Arthritis Rheum. 58 (1): 240-50.
  25. Stolzing, A. et al. (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.
    Mech Ageing Dev. 129 (3): 163-73.
  26. Martinez, C. et al. (2007) Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs.
    Blood. 109 (10): 4245-8.
  27. Jayne, D.G. et al. (1999) A three-dimensional in-vitro model for the study of peritoneal tumour metastasis.
    Clin Exp Metastasis. 17 (6): 515-23.
  28. Lu, G. et al. (2013) Transplantation-potential-related biological properties of decidua basalis mesenchymal stem cells from maternal human term placenta.
    Cell Tissue Res. 352 (2): 301-12.
  29. Pandya, J.M. et al. (2016) CD4+ and CD8+ CD28(null) T Cells Are Cytotoxic to Autologous Muscle Cells in Patients With Polymyositis.
    Arthritis Rheumatol. 68 (8): 2016-26.
  30. Manoussaka, M.S. et al. (2005) Flow cytometric characterisation of cells of differing densities isolated from human term placentae and enrichment of villous trophoblast cells.
    Placenta. 26 (4): 308-18.
  31. Todisco, E. et al. (2002) CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
    Leukemia. 16 (10): 2046-54.
  32. Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
    Cell Transplant. 25 (3): 463-79.
  33. Broeren, M.G.A. (2019) A three-dimensional model to study human synovial pathology
    ALTEX. 36 (1): 18-28.

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