Fibroblasts/Epithelial Cells antibody | D7-FIB
Mouse anti Human Fibroblasts/epithelial cells antibody, clone JB1B used for the detection of CD29 expressing cells in porcine meninges by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis of the porcine meninges. The meninges composed of the dura mater (A–G), the arachnoid mater (H–I) and the pia mater (J–M) in formalin fixed tissue. The porcine spinal cord was cryosectioned and the porcine dura mater (N–O) was stained. The tissue sections were labeled for: (A) von Willebrand factor, (B and K) fibronectin, (C and J) vimentin, (D) laminin, (E) collagen I, (F and M) collagen II, (G, H, and L) integrin 1β, (I) Desmoplakin, (N) collagen III, (O) E-cadherin. Images were captured at ×200 magnification.
From: Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E. Interaction of micron and nano-sized particles with cells of the dura mater. J Biomed Mater Res B Appl Biomater. 2014 Oct;102(7):1496-505.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Fibroblasts/Epithelial Cells
Mouse anti Human Fibroblasts/Epithelial Cells:RPE
- Product Type
- Monoclonal Antibody
- Clone
- D7-FIB
- Isotype
- IgG2a
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA1399GT | C* F IP | 25 µg | ||||
| MCA1399G | C* F IP | 0.2 mg | ||||
| MCA1399PET | F | 25 Tests | ||||
| MCA1399PE | F | 100 Tests |
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody.
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.
In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody.
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes.
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1.0ml distilled water.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Human foreskin fibroblasts.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of Fibroblasts/Epithelial Cells antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen 1 | 1/100 | ||
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for Fibroblasts/Epithelial Cells antibody
-
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Kelynack, K.J. et al. (2000) Human renal fibroblast contraction of collagen I lattices is an integrin-mediated process.
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van Osch, G.J. et al. (2001) Monoclonal antibody 11-fibrau: a useful marker to characterize chondrocyte differentiation stage.
Biochem Biophys Res Commun. 280 (3): 806-12. -
Behl, B. et al. (2013) Biological effects of cobalt-chromium nanoparticles and ions on dural fibroblasts and dural epithelial cells.
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Pountos, I. et al. (2011) NSAIDS inhibit in vitro MSC chondrogenesis but not osteogenesis: implications for mechanism of bone formation inhibition in man.
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Papageorgiou, I. et al. (2014) Interaction of micron and nano-sized particles with cells of the dura mater.
J Biomed Mater Res B Appl Biomater. 102 (7): 1496-505. -
De Bari, C. et al. (2008) A biomarker-based mathematical model to predict bone-forming potency of human synovial and periosteal mesenchymal stem cells.
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