FGF Basic antibody | MC-GF1

Mouse anti Human FGF Basic

Product Type
Monoclonal Antibody
Clone
MC-GF1
Isotype
IgG2a
Specificity
FGF Basic

Product Code Applications Pack Size List Price Your Price Qty
MCA1400G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 0.5 mg loader
List Price Your Price
loader
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Mouse anti Human FGF Basic antibody, clone MC-GF1 recognizes basic fibroblast growth factor (bFGF). Mouse anti Human FGF Basic antibody, clone MC-GF1 binds strongly to bFGF attached to proteoglycans.

Mouse anti Human FGF Basic antibody, clone MC-GF1 is not suitable for detecting bFGF bound to cell surface receptors.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant human bFGF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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References for FGF Basic antibody

  1. Ribatti, D. et al. (2003) Neovascularisation, expression of fibroblast growth factor-2, and mast cells with tryptase activity increase simultaneously with pathological progression in human malignant melanoma.
    Eur J Cancer. 39:666-74.
  2. Whitworth, M.K. et al. (2005) Regulation of fibroblast growth factor-2 activity by human ovarian cancer tumor endothelium.
    Clin Cancer 11: 4282-8.
  3. Zong, F. et al. (2009) Syndecan-1 and FGF-2, but not FGF receptor-1, share a common transport route and co-localize with heparanase in the nuclei of mesenchymal tumor cells.
    PLoS One. 4:e7346.
  4. Melrose, J. et al. (2012) Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development.
    Histochem Cell Biol. 138 (3): 461-75.

Synonyms
FGF2
RRID
AB_2278442
UniProt
P09038
Entrez Gene
FGF2
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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