FGF Basic antibody | MC-GF1
Mouse anti Human FGF basic antibody, clone MC-GF1 (MCA1400G) used for the evaluation of FGF-2 in nuclear extracts of STAV-AB cells inmmunoprecipitated with an anti Syndecan-1 antibody by western blotting.
Image caption:
Syndecan-1 and FGF-2 co-immunoprecipitate in the nucleus of the STAV-AB cells.
Co-IP and immunoblotting were performed on nuclear extracts of sub-confluent cultures of the STAV-AB cells, stably transfected with syndecan-1/EGFP construct or EGFP vector, as described in “Materials and Methods”. Nuclear extract (100 μg) was incubated with 3 μg of syndecan-1 antibody, or with no antibody (negative control). The nuclear precipitates from equal amounts of control and sample proteins were slot-blotted onto a nitrocellulose membrane and then probed with antibody to FGF-2. A crude nuclear extract from syndecan-1/EGFP transfected cells was used as positive control. Co-IP using a specific antibody to syndecan-1 also pulled down FGF-2, as compared to the negative control. The amount of FGF-2 was higher in nuclear extracts from syndecan-1 overexpressing cells, compared to the EGFP control.
From: Zong F, Fthenou E, Wolmer N, Hollósi P, Kovalszky I, Szilák L, et al. (2009)
Syndecan-1 and FGF-2, but Not FGF Receptor-1, Share a Common Transport Route and Co-Localize with Heparanase in the Nuclei of Mesenchymal Tumor Cells.
PLoS ONE 4(10): e7346.
Mouse anti Human FGF basic antibody, clone MC-GF1 (MCA1400G) used for the evaluation of endogenous FGF-2 expression in the nucleus of STAV-AB cells by immunofluorescence.
Image caption:
The sub-cellular localization of exogenously added FGF-2 differs from that of endogenous FGF2.
In the STAV-AB cells, no nuclear accumulation of exogenously added Alexa 488-labeled FGF-2 could be observed 42 hours after seeding (A). In parallel, immunocytochemical staining (B) showed the presence of an endogenous pool of FGF-2 (green) in the nucleus, whereas syndecan-1 (red) remained cytoplasmic at this time point.
From: Zong F, Fthenou E, Wolmer N, Hollósi P, Kovalszky I, Szilák L, et al. (2009)
Syndecan-1 and FGF-2, but Not FGF Receptor-1, Share a Common Transport Route and Co-Localize with Heparanase in the Nuclei of Mesenchymal Tumor Cells.
PLoS ONE 4(10): e7346.
Mouse anti Human FGF Basic antibody, clone MC-GF1 recognizes basic fibroblast growth factor (bFGF). Mouse anti Human FGF Basic antibody, clone MC-GF1 binds strongly to bFGF attached to proteoglycans.
Mouse anti Human FGF Basic antibody, clone MC-GF1 is not suitable for detecting bFGF bound to cell surface receptors.
Mouse anti Human FGF Basic antibody, clone MC-GF1 is not suitable for detecting bFGF bound to cell surface receptors.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant human bFGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P09038 Related reagents
- Entrez Gene
- FGF2 Related reagents
- Regulatory
- For research purposes only
Applications of FGF Basic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Frozen | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for FGF Basic antibody
-
Ribatti, D. et al. (2003) Neovascularisation, expression of fibroblast growth factor-2, and mast cells with tryptase activity increase simultaneously with pathological progression in human malignant melanoma.
Eur J Cancer. 39:666-74. -
Whitworth, M.K. et al. (2005) Regulation of fibroblast growth factor-2 activity by human ovarian cancer tumor endothelium.
Clin Cancer 11: 4282-8. -
Zong, F. et al. (2009) Syndecan-1 and FGF-2, but not FGF receptor-1, share a common transport route and co-localize with heparanase in the nuclei of mesenchymal tumor cells.
PLoS One. 4:e7346. -
Melrose, J. et al. (2012) Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development.
Histochem Cell Biol. 138 (3): 461-75.
