FGF Basic antibody | MC-GF1
Mouse anti Human FGF basic antibody, clone MC-GF1 (MCA1400G) used for the evaluation of FGF-2 in nuclear extracts of STAV-AB cells inmmunoprecipitated with an anti Syndecan-1 antibody by western blotting.
Image caption:
Syndecan-1 and FGF-2 co-immunoprecipitate in the nucleus of the STAV-AB cells.
Co-IP and immunoblotting were performed on nuclear extracts of sub-confluent cultures of the STAV-AB cells, stably transfected with syndecan-1/EGFP construct or EGFP vector, as described in “Materials and Methods”. Nuclear extract (100 μg) was incubated with 3 μg of syndecan-1 antibody, or with no antibody (negative control). The nuclear precipitates from equal amounts of control and sample proteins were slot-blotted onto a nitrocellulose membrane and then probed with antibody to FGF-2. A crude nuclear extract from syndecan-1/EGFP transfected cells was used as positive control. Co-IP using a specific antibody to syndecan-1 also pulled down FGF-2, as compared to the negative control. The amount of FGF-2 was higher in nuclear extracts from syndecan-1 overexpressing cells, compared to the EGFP control.
From: Zong F, Fthenou E, Wolmer N, Hollósi P, Kovalszky I, Szilák L, et al. (2009)
Syndecan-1 and FGF-2, but Not FGF Receptor-1, Share a Common Transport Route and Co-Localize with Heparanase in the Nuclei of Mesenchymal Tumor Cells.
PLoS ONE 4(10): e7346.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human FGF basic antibody, clone MC-GF1 (MCA1400G) used for the evaluation of endogenous FGF-2 expression in the nucleus of STAV-AB cells by immunofluorescence.
Image caption:
The sub-cellular localization of exogenously added FGF-2 differs from that of endogenous FGF2.
In the STAV-AB cells, no nuclear accumulation of exogenously added Alexa 488-labeled FGF-2 could be observed 42 hours after seeding (A). In parallel, immunocytochemical staining (B) showed the presence of an endogenous pool of FGF-2 (green) in the nucleus, whereas syndecan-1 (red) remained cytoplasmic at this time point.
From: Zong F, Fthenou E, Wolmer N, Hollósi P, Kovalszky I, Szilák L, et al. (2009)
Syndecan-1 and FGF-2, but Not FGF Receptor-1, Share a Common Transport Route and Co-Localize with Heparanase in the Nuclei of Mesenchymal Tumor Cells.
PLoS ONE 4(10): e7346.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human FGF Basic
- Product Type
- Monoclonal Antibody
- Clone
- MC-GF1
- Isotype
- IgG2a
- Specificity
- FGF Basic
Antibody Quality and Performance Guarantee
| Mouse anti Human FGF Basic antibody, clone MC-GF1 recognizes basic fibroblast growth factor (bFGF). Mouse anti Human FGF Basic antibody, clone MC-GF1 binds strongly to bFGF attached to proteoglycans. Mouse anti Human FGF Basic antibody, clone MC-GF1 is not suitable for detecting bFGF bound to cell surface receptors. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant human bFGF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Frozen | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for FGF Basic antibody
-
Ribatti, D. et al. (2003) Neovascularisation, expression of fibroblast growth factor-2, and mast cells with tryptase activity increase simultaneously with pathological progression in human malignant melanoma.
Eur J Cancer. 39:666-74. -
Whitworth, M.K. et al. (2005) Regulation of fibroblast growth factor-2 activity by human ovarian cancer tumor endothelium.
Clin Cancer 11: 4282-8. -
Zong, F. et al. (2009) Syndecan-1 and FGF-2, but not FGF receptor-1, share a common transport route and co-localize with heparanase in the nuclei of mesenchymal tumor cells.
PLoS One. 4:e7346. -
Melrose, J. et al. (2012) Chondroitin sulphate and heparan sulphate sulphation motifs and their proteoglycans are involved in articular cartilage formation during human foetal knee joint development.
Histochem Cell Biol. 138 (3): 461-75.
- Synonyms
- FGF2
- RRID
- AB_2278442
- UniProt
- P09038
- Entrez Gene
- FGF2
MCA1400G
0512
150029
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