Factor VIII antibody | RFF-VIIIC/8

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Mouse anti Human Factor VIII

Product Type
Monoclonal Antibody
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4677G E R WB 0.2 mg
MCA4677 E R WB 0.5 mg
Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerizes and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream.

Defects in Factor VIII cause haemophilia A, a disorder characterized by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries.

Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210 kDa, 90 kDa and 40 kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Affinity purified human Factor VIII.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from an immunized Balb/c mouse we fused foth cells of the P3-NS/a-Ag4-1 mouse myeloma.

Storage Information

Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.

More Information

P00451 Related reagents
Entrez Gene
F8 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0006953 acute-phase response
GO:0007155 cell adhesion
GO:0005507 copper ion binding
GO:0005615 extracellular space
GO:0055114 oxidation-reduction process
GO:0016491 oxidoreductase activity
GO:0007597 blood coagulation, intrinsic pathway
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
For research purposes only

Applications of Factor VIII antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/15000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images


Product Specific References

References for Factor VIII antibody

  1. Rotblat, F. et al. (1983) Monoclonal antibodies to human procoagulant factor VIII.
    J Lab Clin Med. 101 (5): 736-46.
  2. Tiarks, C. et al. (1987) Identification of six functional clotting factor VIII:C epitopes by analysis of cross-reactive public idiotypes in murine monoclonal VIII:C inhibitors.
    Thromb Res. 45 (5): 527-37.
  3. Purohit, V.S. et al. (2006) Influence of aggregation on immunogenicity of recombinant human Factor VIII in hemophilia A mice.
    J Pharm Sci. 95 (2): 358-71.
  4. Zaniboni, A. et al. (2015) In vitro differentiation of porcine aortic vascular precursor cells to endothelial and vascular smooth muscle cells.
    Am J Physiol Cell Physiol. 309 (5): C320-31.
  5. Turner NA & Moake JL (2015) Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings.
    PLoS One. 10 (10): e0140740.
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