Factor VIII antibody | RFF-VIIIC/8
Mouse anti Humaqn Factor VIII antibody, clone RFF-VIIIC/8 (MCA4677) used in vitro to inhibit factor VIII activity.
Image caption:
Specificity of FVIII activity assay is demonstrated by antibody inhibition and sample dilutions. (A) FVIII activities, ranging from 0.6–10 mU/ml, in dilutions of rFVIII, were inhibited by addition of 10 μg/ml mouse anti-human FVIII antibody (clone RFF-VIIIC/8) for 10 min (on ice) prior to the start of the chromogenic Coatest assay. The green triangles represent rFVIII without antibody addition and the red squares and blue diamonds are 2 separate rFVIII dilutions with final concentrations of 10 μg/ml anti-FVIII. (B) FVIII activity was measured in HUVEC cell lysates diluted 1.3-, 2-, 3- and 4-fold in 1% BSA/PBS.
From: Turner NA, Moake JL (2015) Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings.
PLoS ONE 10(10): e0140740.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerizes and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream.
Defects in Factor VIII cause haemophilia A, a disorder characterized by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries.
Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210 kDa, 90 kDa and 40 kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.
Defects in Factor VIII cause haemophilia A, a disorder characterized by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries.
Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210 kDa, 90 kDa and 40 kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Mouse Dog Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Affinity purified human Factor VIII.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from an immunized Balb/c mouse we fused foth cells of the P3-NS/a-Ag4-1 mouse myeloma.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P00451 Related reagents
- Entrez Gene
- F8 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0002576 platelet degranulation
- GO:0006953 acute-phase response
- GO:0007155 cell adhesion
- GO:0005507 copper ion binding
- GO:0005615 extracellular space
- GO:0055114 oxidation-reduction process
- GO:0016491 oxidoreductase activity
- GO:0007597 blood coagulation, intrinsic pathway
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- Regulatory
- For research purposes only
Applications of Factor VIII antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/15000 | |
| Radioimmunoassays | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Factor VIII antibody
-
Rotblat, F. et al. (1983) Monoclonal antibodies to human procoagulant factor VIII.
J Lab Clin Med. 101 (5): 736-46. -
Tiarks, C. et al. (1987) Identification of six functional clotting factor VIII:C epitopes by analysis of cross-reactive public idiotypes in murine monoclonal VIII:C inhibitors.
Thromb Res. 45 (5): 527-37. -
Purohit, V.S. et al. (2006) Influence of aggregation on immunogenicity of recombinant human Factor VIII in hemophilia A mice.
J Pharm Sci. 95 (2): 358-71. -
Zaniboni, A. et al. (2015) In vitro differentiation of porcine aortic vascular precursor cells to endothelial and vascular smooth muscle cells.
Am J Physiol Cell Physiol. 309 (5): C320-31. -
Turner NA & Moake JL (2015) Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings.
PLoS One. 10 (10): e0140740.
